Restriction map for
pFN21AE1969
(Linear) (Six-base) MAPSORT of: KIEE1969.seq Check: 8473 from: 1 to: 1602
KIEE1969.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 1602
Size: 285 1317
AccI GT'mk_AC
Cuts at: 0 1115 1602
Size: 1115 487
Acc65I G'GTAC_C
Cuts at: 0 1530 1602
Size: 1530 72
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 1602
Size: 297 1305
AleI CACnn'nnGTG
Cuts at: 0 565 595 1602
Size: 565 30 1007
Fragments arranged by size:
1007 565 30
AlwNI CAG_nnn'CTG
Cuts at: 0 1288 1602
Size: 1288 314
ApoI r'AATT_y
Cuts at: 0 655 1602
Size: 655 947
AvaI C'yCGr_G
Cuts at: 0 1159 1441 1602
Size: 1159 282 161
Fragments arranged by size:
1159 282 161
AvrII C'CTAG_G
Cuts at: 0 1560 1602
Size: 1560 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1514 1547 1602
Size: 1514 33 55
Fragments arranged by size:
1514 55 33
BamHI G'GATC_C
Cuts at: 0 621 1602
Size: 621 981
BanI G'GyrC_C
Cuts at: 0 412 589 1309 1530 1602
Size: 412 177 720 221 72
Fragments arranged by size:
720 412 221 177 72
BanII G_rGCy'C
Cuts at: 0 212 1602
Size: 212 1390
BbvCI CC'TCA_GC
Cuts at: 0 1204 1376 1602
Size: 1204 172 226
Fragments arranged by size:
1204 226 172
BclI T'GATC_A
Cuts at: 0 287 1602
Size: 287 1315
Bme1580I G_kGCm'C
Cuts at: 0 417 1314 1602
Size: 417 897 288
Fragments arranged by size:
897 417 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 932 1602
Size: 932 670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 853 1602
Size: 853 749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1602
Size: 139 1463
Bpu10I CC'TnA_GC
Cuts at: 0 1204 1247 1328 1376 1602
Size: 1204 43 81 48 226
Fragments arranged by size:
1204 226 81 48 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1483 1602
Size: 96 1387 119
Fragments arranged by size:
1387 119 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 1342 1516 1602
Size: 1342 174 86
Fragments arranged by size:
1342 174 86
BsaAI yAC'GTr
Cuts at: 0 385 1406 1488 1602
Size: 385 1021 82 114
Fragments arranged by size:
1021 385 114 82
BsaHI Gr'CG_yC
Cuts at: 0 337 1602
Size: 337 1265
BsaWI w'CCGG_w
Cuts at: 0 1502 1602
Size: 1502 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 898 928 1602
Size: 340 30 528 30 674
Fragments arranged by size:
674 528 340 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 906 1602
Size: 906 696
BseYI C'CCAG_C
Cuts at: 0 958 1263 1602
Size: 958 305 339
Fragments arranged by size:
958 339 305
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 1477 1602
Size: 1300 177 125
Fragments arranged by size:
1300 177 125
BsiHKAI G_wGCw'C
Cuts at: 0 212 1334 1602
Size: 212 1122 268
Fragments arranged by size:
1122 268 212
BsmI GAATG_Cn'
Cuts at: 0 106 1602
Size: 106 1496
BsmBI CGTCTCn'nnnn_
Cuts at: 0 740 899 1192 1602
Size: 740 159 293 410
Fragments arranged by size:
740 410 293 159
Bsp1286I G_dGCh'C
Cuts at: 0 212 417 1314 1334 1602
Size: 212 205 897 20 268
Fragments arranged by size:
897 268 212 205 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 1418 1602
Size: 285 1133 184
Fragments arranged by size:
1133 285 184
BsrFI r'CCGG_y
Cuts at: 0 1394 1423 1602
Size: 1394 29 179
Fragments arranged by size:
1394 179 29
BstBI TT'CG_AA
Cuts at: 0 653 1602
Size: 653 949
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 567 966 1602
Size: 333 234 399 636
Fragments arranged by size:
636 399 333 234
BstYI r'GATC_y
Cuts at: 0 621 1602
Size: 621 981
Bsu36I CC'TnA_GG
Cuts at: 0 617 1602
Size: 617 985
BtgI C'CryG_G
Cuts at: 0 44 875 1304 1429 1602
Size: 44 831 429 125 173
Fragments arranged by size:
831 429 173 125 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1005 1602
Size: 1005 597
BtsI GCAGTG_nn'
Cuts at: 0 636 1477 1602
Size: 636 841 125
Fragments arranged by size:
841 636 125
DraI TTT'AAA
Cuts at: 0 500 1602
Size: 500 1102
EaeI y'GGCC_r
Cuts at: 0 968 1073 1301 1336 1602
Size: 968 105 228 35 266
Fragments arranged by size:
968 266 228 105 35
EarI CTCTTCn'nnn_
Cuts at: 0 395 812 1602
Size: 395 417 790
Fragments arranged by size:
790 417 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 840 1602
Size: 169 671 762
Fragments arranged by size:
762 671 169
EcoICRI GAG'CTC
Cuts at: 0 210 1602
Size: 210 1392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 297 1602
Size: 139 158 1305
Fragments arranged by size:
1305 158 139
EcoO109I rG'GnC_Cy
Cuts at: 0 612 1602
Size: 612 990
EcoRI G'AATT_C
Cuts at: 0 655 1602
Size: 655 947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 1117 1602
Size: 666 451 485
Fragments arranged by size:
666 485 451
FseI GG_CCGG'CC
Cuts at: 0 1427 1602
Size: 1427 175
HaeII r_GCGC'y
Cuts at: 0 1289 1602
Size: 1289 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 898 930 1602
Size: 340 32 526 32 672
Fragments arranged by size:
672 526 340 32 32
HincII GTy'rAC
Cuts at: 0 1475 1602
Size: 1475 127
HindIII A'AGCT_T
Cuts at: 0 1099 1602
Size: 1099 503
KpnI G_GTAC'C
Cuts at: 0 1534 1602
Size: 1534 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1602
Size: 235 1367
MscI TGG'CCA
Cuts at: 0 970 1075 1338 1602
Size: 970 105 263 264
Fragments arranged by size:
970 264 263 105
MslI CAynn'nnrTG
Cuts at: 0 565 595 880 1602
Size: 565 30 285 722
Fragments arranged by size:
722 565 285 30
MspA1I CmG'CkG
Cuts at: 0 1536 1602
Size: 1536 66
NaeI GCC'GGC
Cuts at: 0 1425 1602
Size: 1425 177
NcoI C'CATG_G
Cuts at: 0 44 875 1602
Size: 44 831 727
Fragments arranged by size:
831 727 44
NgoMIV G'CCGG_C
Cuts at: 0 1423 1602
Size: 1423 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 1573 1602
Size: 1573 29
PfoI T'CCnGG_A
Cuts at: 0 848 1602
Size: 848 754
PmlI CAC'GTG
Cuts at: 0 1488 1602
Size: 1488 114
PspXI vC'TCGA_Gb
Cuts at: 0 1159 1602
Size: 1159 443
PstI C_TGCA'G
Cuts at: 0 1156 1541 1602
Size: 1156 385 61
Fragments arranged by size:
1156 385 61
SacI G_AGCT'C
Cuts at: 0 212 1602
Size: 212 1390
SfcI C'TryA_G
Cuts at: 0 1152 1537 1602
Size: 1152 385 65
Fragments arranged by size:
1152 385 65
SmlI C'TyrA_G
Cuts at: 0 111 1159 1462 1602
Size: 111 1048 303 140
Fragments arranged by size:
1048 303 140 111
StuI AGG'CCT
Cuts at: 0 131 975 1006 1169 1602
Size: 131 844 31 163 433
Fragments arranged by size:
844 433 163 131 31
StyI C'CwwG_G
Cuts at: 0 11 44 875 1560 1602
Size: 11 33 831 685 42
Fragments arranged by size:
831 685 42 33 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 914 1297 1602
Size: 914 383 305
Fragments arranged by size:
914 383 305
Tth111I GACn'n_nGTC
Cuts at: 0 898 1602
Size: 898 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 675 1068 1602
Size: 51 624 393 534
Fragments arranged by size:
624 534 393 51
XhoI C'TCGA_G
Cuts at: 0 1159 1602
Size: 1159 443
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlwNI ApoI AvaI
AvrII BaeI BamHI BanI BanII BbvCI BclI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrFI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI HaeII Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PmlI PspXI PstI SacI SfcI SmlI
StuI StyI TaqII Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BbeI BbsI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BsaBI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I ClaI CspCI DraIII
DrdI EagI EcoNI EcoRV FspI FspAI HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI XbaI XmaI XmnI ZraI