Restriction map for
pFN21AE1968
(Linear) (Six-base) MAPSORT of: KIEE1968.seq Check: 9252 from: 1 to: 1593
KIEE1968.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1474 1593
Size: 1474 119
AatII G_ACGT'C
Cuts at: 0 1282 1593
Size: 1282 311
AccI GT'mk_AC
Cuts at: 0 1073 1593
Size: 1073 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 536 1593
Size: 200 336 1057
Fragments arranged by size:
1057 336 200
AflII C'TTAA_G
Cuts at: 0 292 1593
Size: 292 1301
AgeI A'CCGG_T
Cuts at: 0 420 1593
Size: 420 1173
AlwNI CAG_nnn'CTG
Cuts at: 0 602 1204 1593
Size: 602 602 389
Fragments arranged by size:
602 602 389
ApaI G_GGCC'C
Cuts at: 0 1417 1593
Size: 1417 176
ApaLI G'TGCA_C
Cuts at: 0 1341 1593
Size: 1341 252
ApoI r'AATT_y
Cuts at: 0 253 279 318 800 1297 1593
Size: 253 26 39 482 497 296
Fragments arranged by size:
497 482 296 253 39 26
BanII G_rGCy'C
Cuts at: 0 1417 1593
Size: 1417 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 1234 1593
Size: 1234 359
BciVI GTATCCnnnnn_n'
Cuts at: 0 256 677 1593
Size: 256 421 916
Fragments arranged by size:
916 421 256
BclI T'GATC_A
Cuts at: 0 852 1593
Size: 852 741
BglI GCCn_nnn'nGGC
Cuts at: 0 526 1593
Size: 526 1067
BglII A'GATC_T
Cuts at: 0 117 408 1593
Size: 117 291 1185
Fragments arranged by size:
1185 291 117
Bme1580I G_kGCm'C
Cuts at: 0 507 1345 1417 1593
Size: 507 838 72 176
Fragments arranged by size:
838 507 176 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 328 1593
Size: 328 1265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1573 1593
Size: 619 954 20
Fragments arranged by size:
954 619 20
Bpu10I CC'TnA_GC
Cuts at: 0 569 1593
Size: 569 1024
BsaI GGTCTCn'nnnn_
Cuts at: 0 1591 1593
Size: 1591 2
BsaAI yAC'GTr
Cuts at: 0 538 1593
Size: 538 1055
BsaBI GATnn'nnATC
Cuts at: 0 116 1593
Size: 116 1477
BsaHI Gr'CG_yC
Cuts at: 0 1279 1593
Size: 1279 314
BsaWI w'CCGG_w
Cuts at: 0 420 1593
Size: 420 1173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1055 1085 1593
Size: 1055 30 508
Fragments arranged by size:
1055 508 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1593
Size: 67 1526
BseYI C'CCAG_C
Cuts at: 0 826 1593
Size: 826 767
BsiEI CG_ry'CG
Cuts at: 0 33 1593
Size: 33 1560
BsiHKAI G_wGCw'C
Cuts at: 0 7 537 1345 1426 1593
Size: 7 530 808 81 167
Fragments arranged by size:
808 530 167 81 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 507 537 1345 1417 1426 1593
Size: 7 500 30 808 72 9 167
Fragments arranged by size:
808 500 167 72 30 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 610 1474 1593
Size: 68 542 864 119
Fragments arranged by size:
864 542 119 68
BsrDI GCAATG_nn'
Cuts at: 0 623 884 1593
Size: 623 261 709
Fragments arranged by size:
709 623 261
BsrFI r'CCGG_y
Cuts at: 0 25 420 1593
Size: 25 395 1173
Fragments arranged by size:
1173 395 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 511 1593
Size: 511 1082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 341 1593
Size: 341 1252
BstYI r'GATC_y
Cuts at: 0 117 408 543 1221 1530 1593
Size: 117 291 135 678 309 63
Fragments arranged by size:
678 309 291 135 117 63
BtgI C'CryG_G
Cuts at: 0 126 1593
Size: 126 1467
BtsI GCAGTG_nn'
Cuts at: 0 1112 1593
Size: 1112 481
EaeI y'GGCC_r
Cuts at: 0 30 527 1593
Size: 30 497 1066
Fragments arranged by size:
1066 497 30
EagI C'GGCC_G
Cuts at: 0 30 1593
Size: 30 1563
EarI CTCTTCn'nnn_
Cuts at: 0 347 1593
Size: 347 1246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 536 619 1573 1593
Size: 200 336 83 954 20
Fragments arranged by size:
954 336 200 83 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 1593
Size: 637 956
EcoO109I rG'GnC_Cy
Cuts at: 0 57 1414 1593
Size: 57 1357 179
Fragments arranged by size:
1357 179 57
EcoRI G'AATT_C
Cuts at: 0 318 800 1593
Size: 318 482 793
Fragments arranged by size:
793 482 318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 400 967 1593
Size: 400 567 626
Fragments arranged by size:
626 567 400
HaeII r_GCGC'y
Cuts at: 0 23 41 1593
Size: 23 18 1552
Fragments arranged by size:
1552 23 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 1593
Size: 1055 32 506
Fragments arranged by size:
1055 506 32
MfeI C'AATT_G
Cuts at: 0 1349 1593
Size: 1349 244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1593
Size: 53 1540
MscI TGG'CCA
Cuts at: 0 529 1593
Size: 529 1064
MslI CAynn'nnrTG
Cuts at: 0 242 590 1338 1593
Size: 242 348 748 255
Fragments arranged by size:
748 348 255 242
MspA1I CmG'CkG
Cuts at: 0 80 1593
Size: 80 1513
NdeI CA'TA_TG
Cuts at: 0 491 1593
Size: 491 1102
NspI r_CATG'y
Cuts at: 0 1129 1593
Size: 1129 464
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 1593
Size: 879 714
PfoI T'CCnGG_A
Cuts at: 0 635 1593
Size: 635 958
PmlI CAC'GTG
Cuts at: 0 538 1593
Size: 538 1055
PpuMI rG'GwC_Cy
Cuts at: 0 57 1593
Size: 57 1536
PsiI TTA'TAA
Cuts at: 0 889 1593
Size: 889 704
PspOMI G'GGCC_C
Cuts at: 0 1413 1593
Size: 1413 180
SfcI C'TryA_G
Cuts at: 0 302 956 1593
Size: 302 654 637
Fragments arranged by size:
654 637 302
SmlI C'TyrA_G
Cuts at: 0 292 1593
Size: 292 1301
SpeI A'CTAG_T
Cuts at: 0 616 1593
Size: 616 977
SspI AAT'ATT
Cuts at: 0 222 1593
Size: 222 1371
StyI C'CwwG_G
Cuts at: 0 340 1593
Size: 340 1253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 776 1059 1593
Size: 776 283 534
Fragments arranged by size:
776 534 283
TatI w'GTAC_w
Cuts at: 0 311 1593
Size: 311 1282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 1593
Size: 772 32 789
Fragments arranged by size:
789 772 32
XmnI GAAnn'nnTTC
Cuts at: 0 944 1593
Size: 944 649
ZraI GAC'GTC
Cuts at: 0 1280 1593
Size: 1280 313
Enzymes that do cut:
AarI AatII AccI AcuI AflII AgeI AlwNI ApaI
ApaLI ApoI BanII BbsI BciVI BclI BglI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI EaeI
EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I MfeI MmeI MscI MslI MspA1I NdeI NspI
PflMI PfoI PmlI PpuMI PsiI PspOMI SfcI SmlI
SpeI SspI StyI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AhdI AleI AlfI AloI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BfrBI BlpI BmgBI BmtI BplI
BpuEI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI