Restriction map for
pFN21AE1967
(Linear) (Six-base) MAPSORT of: KIEE1967.seq Check: 3208 from: 1 to: 1590
KIEE1967.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AfeI AGC'GCT
Cuts at: 0 663 1590
Size: 663 927
AflIII A'CryG_T
Cuts at: 0 503 1590
Size: 503 1087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1585 1590
Size: 1585 5
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1590
Size: 861 729
AvaI C'yCGr_G
Cuts at: 0 30 461 562 1383 1590
Size: 30 431 101 821 207
Fragments arranged by size:
821 431 207 101 30
BamHI G'GATC_C
Cuts at: 0 1022 1590
Size: 1022 568
BanI G'GyrC_C
Cuts at: 0 104 277 311 466 942 1129 1590
Size: 104 173 34 155 476 187 461
Fragments arranged by size:
476 461 187 173 155 104 34
BanII G_rGCy'C
Cuts at: 0 24 1089 1345 1390 1590
Size: 24 1065 256 45 200
Fragments arranged by size:
1065 256 200 45 24
BbeI G_GCGC'C
Cuts at: 0 108 315 946 1133 1590
Size: 108 207 631 187 457
Fragments arranged by size:
631 457 207 187 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 681 915 1590
Size: 681 234 675
Fragments arranged by size:
681 675 234
BbvCI CC'TCA_GC
Cuts at: 0 1558 1590
Size: 1558 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 792 826 1590
Size: 792 34 764
Fragments arranged by size:
792 764 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 623 1590
Size: 623 967
BglI GCCn_nnn'nGGC
Cuts at: 0 400 591 710 1432 1590
Size: 400 191 119 722 158
Fragments arranged by size:
722 400 191 158 119
BlpI GC'TnA_GC
Cuts at: 0 658 1590
Size: 658 932
Bme1580I G_kGCm'C
Cuts at: 0 84 469 850 1590
Size: 84 385 381 740
Fragments arranged by size:
740 385 381 84
BmgBI CAC'GTC
Cuts at: 0 930 1590
Size: 930 660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 431 1590
Size: 37 394 1159
Fragments arranged by size:
1159 394 37
Bpu10I CC'TnA_GC
Cuts at: 0 1558 1590
Size: 1558 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1590
Size: 958 632
BsaI GGTCTCn'nnnn_
Cuts at: 0 950 1070 1283 1590
Size: 950 120 213 307
Fragments arranged by size:
950 307 213 120
BsaAI yAC'GTr
Cuts at: 0 504 515 1590
Size: 504 11 1075
Fragments arranged by size:
1075 504 11
BsaHI Gr'CG_yC
Cuts at: 0 105 114 312 476 623 943 1130 1590
Size: 105 9 198 164 147 320 187 460
Fragments arranged by size:
460 320 198 187 164 147 105 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1280 1310 1590
Size: 1280 30 280
Fragments arranged by size:
1280 280 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 889 892 1380 1383 1501 1558 1590
Size: 889 3 488 3 118 57 32
Fragments arranged by size:
889 488 118 57 32 3 3
BseYI C'CCAG_C
Cuts at: 0 273 305 738 1590
Size: 273 32 433 852
Fragments arranged by size:
852 433 273 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1590
Size: 1400 190
BsiEI CG_ry'CG
Cuts at: 0 332 831 1590
Size: 332 499 759
Fragments arranged by size:
759 499 332
BsiHKAI G_wGCw'C
Cuts at: 0 24 203 348 778 1089 1590
Size: 24 179 145 430 311 501
Fragments arranged by size:
501 430 311 179 145 24
Bsp1286I G_dGCh'C
Cuts at: 0 24 84 203 348 469 778 850 1089
Size: 24 60 119 145 121 309 72 239
Cuts at: 1089 1345 1390 1590
Size: 256 45 200
Fragments arranged by size:
309 256 239 200 145 121 119 72
60 45 24
BsrBI CCG'CTC
Cuts at: 0 1028 1527 1590
Size: 1028 499 63
Fragments arranged by size:
1028 499 63
BsrDI GCAATG_nn'
Cuts at: 0 459 1428 1590
Size: 459 969 162
Fragments arranged by size:
969 459 162
BssHII G'CGCG_C
Cuts at: 0 35 1590
Size: 35 1555
BstAPI GCAn_nnn'nTGC
Cuts at: 0 845 1423 1590
Size: 845 578 167
Fragments arranged by size:
845 578 167
BstXI CCAn_nnnn'nTGG
Cuts at: 0 768 1126 1590
Size: 768 358 464
Fragments arranged by size:
768 464 358
BstYI r'GATC_y
Cuts at: 0 1022 1590
Size: 1022 568
Bsu36I CC'TnA_GG
Cuts at: 0 1541 1590
Size: 1541 49
BtgI C'CryG_G
Cuts at: 0 416 707 1035 1112 1505 1590
Size: 416 291 328 77 393 85
Fragments arranged by size:
416 393 328 291 85 77
BtsI GCAGTG_nn'
Cuts at: 0 100 187 259 946 1590
Size: 100 87 72 687 644
Fragments arranged by size:
687 644 100 87 72
DraIII CAC_nnn'GTG
Cuts at: 0 515 990 1590
Size: 515 475 600
Fragments arranged by size:
600 515 475
DrdI GACnn_nn'nnGTC
Cuts at: 0 685 934 976 1590
Size: 685 249 42 614
Fragments arranged by size:
685 614 249 42
EaeI y'GGCC_r
Cuts at: 0 329 1508 1590
Size: 329 1179 82
Fragments arranged by size:
1179 329 82
EagI C'GGCC_G
Cuts at: 0 329 1590
Size: 329 1261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1464 1590
Size: 1464 126
EcoICRI GAG'CTC
Cuts at: 0 22 1087 1590
Size: 22 1065 503
Fragments arranged by size:
1065 503 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 431 1590
Size: 37 394 1159
Fragments arranged by size:
1159 394 37
EcoO109I rG'GnC_Cy
Cuts at: 0 1278 1590
Size: 1278 312
FspI TGC'GCA
Cuts at: 0 1069 1590
Size: 1069 521
HaeII r_GCGC'y
Cuts at: 0 108 166 315 522 665 861 946 1133
Size: 108 58 149 207 143 196 85 187
Cuts at: 1133 1181 1590
Size: 48 409
Fragments arranged by size:
409 207 196 187 149 143 108 85
58 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 1590
Size: 1280 32 278
Fragments arranged by size:
1280 278 32
HincII GTy'rAC
Cuts at: 0 1533 1590
Size: 1533 57
HindIII A'AGCT_T
Cuts at: 0 1123 1590
Size: 1123 467
KasI G'GCGC_C
Cuts at: 0 104 311 942 1129 1590
Size: 104 207 631 187 461
Fragments arranged by size:
631 461 207 187 104
MspA1I CmG'CkG
Cuts at: 0 119 246 273 286 305 388 418 495
Size: 119 127 27 13 19 83 30 77
Cuts at: 495 674 709 716 1114 1590
Size: 179 35 7 398 476
Fragments arranged by size:
476 398 179 127 119 83 77 35
30 27 19 13 7
NarI GG'CG_CC
Cuts at: 0 105 312 943 1130 1590
Size: 105 207 631 187 460
Fragments arranged by size:
631 460 207 187 105
NcoI C'CATG_G
Cuts at: 0 1505 1590
Size: 1505 85
NspI r_CATG'y
Cuts at: 0 598 657 1098 1590
Size: 598 59 441 492
Fragments arranged by size:
598 492 441 59
PasI CC'CwG_GG
Cuts at: 0 316 1590
Size: 316 1274
PflMI CCAn_nnn'nTGG
Cuts at: 0 454 1173 1260 1445 1506 1590
Size: 454 719 87 185 61 84
Fragments arranged by size:
719 454 185 87 84 61
PfoI T'CCnGG_A
Cuts at: 0 218 1590
Size: 218 1372
PmlI CAC'GTG
Cuts at: 0 515 1590
Size: 515 1075
PpuMI rG'GwC_Cy
Cuts at: 0 1278 1590
Size: 1278 312
PstI C_TGCA'G
Cuts at: 0 69 251 414 638 1220 1232 1250 1590
Size: 69 182 163 224 582 12 18 340
Fragments arranged by size:
582 340 224 182 163 69 18 12
PvuII CAG'CTG
Cuts at: 0 305 495 1590
Size: 305 190 1095
Fragments arranged by size:
1095 305 190
RsrII CG'GwC_CG
Cuts at: 0 1451 1590
Size: 1451 139
SacI G_AGCT'C
Cuts at: 0 24 1089 1590
Size: 24 1065 501
Fragments arranged by size:
1065 501 24
SacII CC_GC'GG
Cuts at: 0 419 710 1115 1590
Size: 419 291 405 475
Fragments arranged by size:
475 419 405 291
SbfI CC_TGCA'GG
Cuts at: 0 638 1590
Size: 638 952
SexAI A'CCwGG_T
Cuts at: 0 1259 1280 1590
Size: 1259 21 310
Fragments arranged by size:
1259 310 21
SfcI C'TryA_G
Cuts at: 0 65 247 410 634 1057 1216 1228 1246
Size: 65 182 163 224 423 159 12 18
Cuts at: 1246 1590
Size: 344
Fragments arranged by size:
423 344 224 182 163 159 65 18
12
SfoI GGC'GCC
Cuts at: 0 106 313 944 1131 1590
Size: 106 207 631 187 459
Fragments arranged by size:
631 459 207 187 106
SmaI CCC'GGG
Cuts at: 0 32 1590
Size: 32 1558
SmlI C'TyrA_G
Cuts at: 0 973 1590
Size: 973 617
SpeI A'CTAG_T
Cuts at: 0 900 1590
Size: 900 690
SphI G_CATG'C
Cuts at: 0 1098 1590
Size: 1098 492
StyI C'CwwG_G
Cuts at: 0 1505 1590
Size: 1505 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 1590
Size: 265 1325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 214 246 1322 1354 1590
Size: 131 32 51 32 1076 32 236
Fragments arranged by size:
1076 236 131 51 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1294 1590
Size: 1294 296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 116 765 1404 1512 1590
Size: 116 649 639 108 78
Fragments arranged by size:
649 639 116 108 78
XmaI C'CCGG_G
Cuts at: 0 30 1590
Size: 30 1560
XmnI GAAnn'nnTTC
Cuts at: 0 148 1590
Size: 148 1442
Enzymes that do cut:
AfeI AflIII AlfI AlwNI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I FspI
HaeII Hin4I HincII HindIII KasI MspA1I NarI NcoI
NspI PasI PflMI PfoI PmlI PpuMI PstI PvuII
RsrII SacI SacII SbfI SexAI SfcI SfoI SmaI
SmlI SpeI SphI StyI TaqII TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BclI BfrBI BglII BmrI BmtI
BplI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrFI BsrGI BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI EarI EcoNI EcoRI EcoRV FalI
FseI FspAI HpaI KpnI MfeI MluI MmeI MscI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI SalI SanDI SapI ScaI SfiI SgfI
SgrAI SnaBI SrfI SspI StuI SwaI TatI XbaI
XhoI ZraI