Restriction map for
pFN21AE1964
(Linear) (Six-base) MAPSORT of: KIEE1964.seq Check: 8887 from: 1 to: 1584
KIEE1964.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 87 445 455 1584
Size: 87 358 10 1129
Fragments arranged by size:
1129 358 87 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 211 970 1396 1479 1584
Size: 88 123 759 426 83 105
Fragments arranged by size:
759 426 123 105 88 83
AfeI AGC'GCT
Cuts at: 0 777 994 1584
Size: 777 217 590
Fragments arranged by size:
777 590 217
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 865 897 1584
Size: 865 32 687
Fragments arranged by size:
865 687 32
AlwNI CAG_nnn'CTG
Cuts at: 0 369 546 1584
Size: 369 177 1038
Fragments arranged by size:
1038 369 177
ApaI G_GGCC'C
Cuts at: 0 114 1427 1584
Size: 114 1313 157
Fragments arranged by size:
1313 157 114
AscI GG'CGCG_CC
Cuts at: 0 87 1584
Size: 87 1497
AvaI C'yCGr_G
Cuts at: 0 608 1147 1426 1584
Size: 608 539 279 158
Fragments arranged by size:
608 539 279 158
BanI G'GyrC_C
Cuts at: 0 478 831 1584
Size: 478 353 753
Fragments arranged by size:
753 478 353
BanII G_rGCy'C
Cuts at: 0 114 188 1184 1252 1312 1427 1435 1584
Size: 114 74 996 68 60 115 8 149
Fragments arranged by size:
996 149 115 114 74 68 60 8
BbeI G_GCGC'C
Cuts at: 0 482 1584
Size: 482 1102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 724 758 1584
Size: 724 34 826
Fragments arranged by size:
826 724 34
BglI GCCn_nnn'nGGC
Cuts at: 0 610 1102 1172 1584
Size: 610 492 70 412
Fragments arranged by size:
610 492 412 70
BlpI GC'TnA_GC
Cuts at: 0 1306 1445 1584
Size: 1306 139 139
Fragments arranged by size:
1306 139 139
Bme1580I G_kGCm'C
Cuts at: 0 114 836 1427 1584
Size: 114 722 591 157
Fragments arranged by size:
722 591 157 114
BmgBI CAC'GTC
Cuts at: 0 711 1584
Size: 711 873
BmrI ACTGGGnnnn_n'
Cuts at: 0 206 831 1249 1584
Size: 206 625 418 335
Fragments arranged by size:
625 418 335 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 247 391 577 964 1002 1584
Size: 34 213 144 186 387 38 582
Fragments arranged by size:
582 387 213 186 144 38 34
Bpu10I CC'TnA_GC
Cuts at: 0 772 779 1183 1584
Size: 772 7 404 401
Fragments arranged by size:
772 404 401 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 1584
Size: 1507 77
BsaHI Gr'CG_yC
Cuts at: 0 415 479 898 965 1584
Size: 415 64 419 67 619
Fragments arranged by size:
619 419 415 67 64
BsaWI w'CCGG_w
Cuts at: 0 644 1286 1584
Size: 644 642 298
Fragments arranged by size:
644 642 298
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 937 1216 1575 1584
Size: 937 279 359 9
Fragments arranged by size:
937 359 279 9
BseYI C'CCAG_C
Cuts at: 0 92 376 794 1064 1478 1584
Size: 92 284 418 270 414 106
Fragments arranged by size:
418 414 284 270 106 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 379 433 691 790 1584
Size: 307 72 54 258 99 794
Fragments arranged by size:
794 307 258 99 72 54
BsiEI CG_ry'CG
Cuts at: 0 576 1584
Size: 576 1008
BsiHKAI G_wGCw'C
Cuts at: 0 11 55 1184 1584
Size: 11 44 1129 400
Fragments arranged by size:
1129 400 44 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 889 1584
Size: 889 695
Bsp1286I G_dGCh'C
Cuts at: 0 11 55 114 188 836 1184 1252 1312
Size: 11 44 59 74 648 348 68 60
Cuts at: 1312 1427 1435 1584
Size: 115 8 149
Fragments arranged by size:
648 348 149 115 74 68 60 59
44 11 8
BspEI T'CCGG_A
Cuts at: 0 644 1584
Size: 644 940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 87 186 445 455 1584
Size: 87 99 259 10 1129
Fragments arranged by size:
1129 259 99 87 10
BsrBI CCG'CTC
Cuts at: 0 63 1584
Size: 63 1521
BsrFI r'CCGG_y
Cuts at: 0 650 1024 1584
Size: 650 374 560
Fragments arranged by size:
650 560 374
BssHII G'CGCG_C
Cuts at: 0 87 975 1584
Size: 87 888 609
Fragments arranged by size:
888 609 87
BssSI C'ACGA_G
Cuts at: 0 850 1584
Size: 850 734
BstEII G'GTnAC_C
Cuts at: 0 1470 1584
Size: 1470 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 271 489 942 1584
Size: 271 218 453 642
Fragments arranged by size:
642 453 271 218
Bsu36I CC'TnA_GG
Cuts at: 0 1418 1584
Size: 1418 166
BtgI C'CryG_G
Cuts at: 0 35 566 576 690 812 1032 1553 1584
Size: 35 531 10 114 122 220 521 31
Fragments arranged by size:
531 521 220 122 114 35 31 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 453 1584
Size: 453 1131
DraIII CAC_nnn'GTG
Cuts at: 0 456 1584
Size: 456 1128
DrdI GACnn_nn'nnGTC
Cuts at: 0 1291 1584
Size: 1291 293
EaeI y'GGCC_r
Cuts at: 0 2 296 431 441 569 1236 1584
Size: 2 294 135 10 128 667 348
Fragments arranged by size:
667 348 294 135 128 10 2
EarI CTCTTCn'nnn_
Cuts at: 0 208 1584
Size: 208 1376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 1584
Size: 250 1334
EcoICRI GAG'CTC
Cuts at: 0 1182 1584
Size: 1182 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 88 211 247 391 577 964 970
Size: 34 54 123 36 144 186 387 6
Cuts at: 970 1002 1396 1479 1584
Size: 32 394 83 105
Fragments arranged by size:
394 387 186 144 123 105 83 54
36 34 32 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 226 1584
Size: 193 33 1358
Fragments arranged by size:
1358 193 33
EcoO109I rG'GnC_Cy
Cuts at: 0 145 198 1423 1509 1584
Size: 145 53 1225 86 75
Fragments arranged by size:
1225 145 86 75 53
FspI TGC'GCA
Cuts at: 0 628 1584
Size: 628 956
HaeII r_GCGC'y
Cuts at: 0 482 779 996 1025 1383 1584
Size: 482 297 217 29 358 201
Fragments arranged by size:
482 358 297 217 201 29
HincII GTy'rAC
Cuts at: 0 498 1584
Size: 498 1086
KasI G'GCGC_C
Cuts at: 0 478 1584
Size: 478 1106
MscI TGG'CCA
Cuts at: 0 298 1584
Size: 298 1286
MspA1I CmG'CkG
Cuts at: 0 235 804 814 1034 1305 1444 1584
Size: 235 569 10 220 271 139 140
Fragments arranged by size:
569 271 235 220 140 139 10
NaeI GCC'GGC
Cuts at: 0 652 1026 1584
Size: 652 374 558
Fragments arranged by size:
652 558 374
NarI GG'CG_CC
Cuts at: 0 479 1584
Size: 479 1105
NcoI C'CATG_G
Cuts at: 0 35 566 1584
Size: 35 531 1018
Fragments arranged by size:
1018 531 35
NgoMIV G'CCGG_C
Cuts at: 0 650 1024 1584
Size: 650 374 560
Fragments arranged by size:
650 560 374
NspI r_CATG'y
Cuts at: 0 76 427 516 633 1584
Size: 76 351 89 117 951
Fragments arranged by size:
951 351 117 89 76
PasI CC'CwG_GG
Cuts at: 0 132 1584
Size: 132 1452
PflMI CCAn_nnn'nTGG
Cuts at: 0 1112 1338 1365 1584
Size: 1112 226 27 219
Fragments arranged by size:
1112 226 219 27
PfoI T'CCnGG_A
Cuts at: 0 368 1584
Size: 368 1216
PpuMI rG'GwC_Cy
Cuts at: 0 145 198 1509 1584
Size: 145 53 1311 75
Fragments arranged by size:
1311 145 75 53
PspOMI G'GGCC_C
Cuts at: 0 110 1423 1584
Size: 110 1313 161
Fragments arranged by size:
1313 161 110
PstI C_TGCA'G
Cuts at: 0 659 1406 1584
Size: 659 747 178
Fragments arranged by size:
747 659 178
PvuII CAG'CTG
Cuts at: 0 1305 1444 1584
Size: 1305 139 140
Fragments arranged by size:
1305 140 139
SacI G_AGCT'C
Cuts at: 0 1184 1584
Size: 1184 400
SacII CC_GC'GG
Cuts at: 0 815 1035 1584
Size: 815 220 549
Fragments arranged by size:
815 549 220
SanDI GG'GwC_CC
Cuts at: 0 1509 1584
Size: 1509 75
SexAI A'CCwGG_T
Cuts at: 0 452 1584
Size: 452 1132
SfcI C'TryA_G
Cuts at: 0 655 1402 1584
Size: 655 747 182
Fragments arranged by size:
747 655 182
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 610 1584
Size: 610 974
SfoI GGC'GCC
Cuts at: 0 480 1584
Size: 480 1104
SmaI CCC'GGG
Cuts at: 0 610 1584
Size: 610 974
SmlI C'TyrA_G
Cuts at: 0 1522 1584
Size: 1522 62
SphI G_CATG'C
Cuts at: 0 76 427 633 1584
Size: 76 351 206 951
Fragments arranged by size:
951 351 206 76
SrfI GCCC'GGGC
Cuts at: 0 610 1584
Size: 610 974
StyI C'CwwG_G
Cuts at: 0 35 180 270 566 1584
Size: 35 145 90 296 1018
Fragments arranged by size:
1018 296 145 90 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 601 1584
Size: 601 983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1486 1584
Size: 1486 98
XmaI C'CCGG_G
Cuts at: 0 608 1584
Size: 608 976
Enzymes that do cut:
AarI AcuI AfeI AloI AlwNI ApaI AscI AvaI
BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BssSI BstEII BstXI Bsu36I BtgI BtgZI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII HincII KasI MscI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SrfI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI
BplI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BspHI
BsrDI BsrGI BstAPI BstBI BstYI BstZ17I BtsI ClaI
CspCI DraI EagI EcoRI EcoRV FalI FseI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI MslI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI