Restriction map for
pFN21AE1960
(Linear) (Six-base) MAPSORT of: KIEE1960.seq Check: 1501 from: 1 to: 1566
KIEE1960.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AccI GT'mk_AC
Cuts at: 0 602 1091 1566
Size: 602 489 475
Fragments arranged by size:
602 489 475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1417 1566
Size: 910 507 149
Fragments arranged by size:
910 507 149
AlwNI CAG_nnn'CTG
Cuts at: 0 1383 1566
Size: 1383 183
ApaLI G'TGCA_C
Cuts at: 0 740 1566
Size: 740 826
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 515 548 1566
Size: 515 33 1018
Fragments arranged by size:
1018 515 33
BanI G'GyrC_C
Cuts at: 0 1263 1566
Size: 1263 303
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 1427 1566
Size: 227 1200 139
Fragments arranged by size:
1200 227 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1225 1259 1566
Size: 1225 34 307
Fragments arranged by size:
1225 307 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1350 1566
Size: 1350 216
BlpI GC'TnA_GC
Cuts at: 0 168 1566
Size: 168 1398
Bme1580I G_kGCm'C
Cuts at: 0 744 1159 1547 1566
Size: 744 415 388 19
Fragments arranged by size:
744 415 388 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 529 741 1566
Size: 175 354 212 825
Fragments arranged by size:
825 354 212 175
Bpu10I CC'TnA_GC
Cuts at: 0 1256 1566
Size: 1256 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1566
Size: 100 1466
BsaI GGTCTCn'nnnn_
Cuts at: 0 759 1566
Size: 759 807
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1117 1147 1566
Size: 1117 30 419
Fragments arranged by size:
1117 419 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 1566
Size: 235 1331
BseYI C'CCAG_C
Cuts at: 0 1502 1566
Size: 1502 64
BsiHKAI G_wGCw'C
Cuts at: 0 744 1566
Size: 744 822
Bsp1286I G_dGCh'C
Cuts at: 0 744 1159 1547 1566
Size: 744 415 388 19
Fragments arranged by size:
744 415 388 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 700 1566
Size: 359 341 866
Fragments arranged by size:
866 359 341
BsrGI T'GTAC_A
Cuts at: 0 824 1566
Size: 824 742
BstXI CCAn_nnnn'nTGG
Cuts at: 0 949 1566
Size: 949 617
BstYI r'GATC_y
Cuts at: 0 204 923 1566
Size: 204 719 643
Fragments arranged by size:
719 643 204
BtgI C'CryG_G
Cuts at: 0 1071 1566
Size: 1071 495
BtsI GCAGTG_nn'
Cuts at: 0 1159 1439 1566
Size: 1159 280 127
Fragments arranged by size:
1159 280 127
EaeI y'GGCC_r
Cuts at: 0 1319 1566
Size: 1319 247
EarI CTCTTCn'nnn_
Cuts at: 0 1101 1205 1461 1566
Size: 1101 104 256 105
Fragments arranged by size:
1101 256 105 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 529 741 910 1417 1566
Size: 175 354 212 169 507 149
Fragments arranged by size:
507 354 212 175 169 149
EcoO109I rG'GnC_Cy
Cuts at: 0 513 1107 1566
Size: 513 594 459
Fragments arranged by size:
594 513 459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 439 684 1566
Size: 439 245 882
Fragments arranged by size:
882 439 245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 915 947 1117 1119 1149 1151 1414 1446
Size: 915 32 170 2 30 2 263 32
Cuts at: 1446 1566
Size: 120
Fragments arranged by size:
915 263 170 120 32 32 30 2
2
HincII GTy'rAC
Cuts at: 0 16 867 1449 1566
Size: 16 851 582 117
Fragments arranged by size:
851 582 117 16
HindIII A'AGCT_T
Cuts at: 0 67 1566
Size: 67 1499
MscI TGG'CCA
Cuts at: 0 1321 1566
Size: 1321 245
MslI CAynn'nnrTG
Cuts at: 0 914 974 1566
Size: 914 60 592
Fragments arranged by size:
914 592 60
MspA1I CmG'CkG
Cuts at: 0 820 1367 1566
Size: 820 547 199
Fragments arranged by size:
820 547 199
NcoI C'CATG_G
Cuts at: 0 1071 1566
Size: 1071 495
NspI r_CATG'y
Cuts at: 0 506 709 1354 1566
Size: 506 203 645 212
Fragments arranged by size:
645 506 212 203
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 1566
Size: 105 1461
PpuMI rG'GwC_Cy
Cuts at: 0 513 1566
Size: 513 1053
PstI C_TGCA'G
Cuts at: 0 278 1010 1154 1280 1566
Size: 278 732 144 126 286
Fragments arranged by size:
732 286 278 144 126
PvuII CAG'CTG
Cuts at: 0 820 1367 1566
Size: 820 547 199
Fragments arranged by size:
820 547 199
SbfI CC_TGCA'GG
Cuts at: 0 1280 1566
Size: 1280 286
SfcI C'TryA_G
Cuts at: 0 274 599 1006 1150 1276 1566
Size: 274 325 407 144 126 290
Fragments arranged by size:
407 325 290 274 144 126
SmlI C'TyrA_G
Cuts at: 0 8 115 1566
Size: 8 107 1451
Fragments arranged by size:
1451 107 8
SpeI A'CTAG_T
Cuts at: 0 639 1530 1566
Size: 639 891 36
Fragments arranged by size:
891 639 36
SphI G_CATG'C
Cuts at: 0 709 1566
Size: 709 857
StuI AGG'CCT
Cuts at: 0 851 1198 1566
Size: 851 347 368
Fragments arranged by size:
851 368 347
StyI C'CwwG_G
Cuts at: 0 1071 1203 1566
Size: 1071 132 363
Fragments arranged by size:
1071 363 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1281 1566
Size: 1281 285
TatI w'GTAC_w
Cuts at: 0 824 1566
Size: 824 742
XbaI T'CTAG_A
Cuts at: 0 465 1566
Size: 465 1101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 300 1078 1566
Size: 150 150 778 488
Fragments arranged by size:
778 488 150 150
Enzymes that do cut:
AccI AcuI AlwNI ApaLI BaeI BanI BbsI BcgI
BciVI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrGI BstXI BstYI
BtgI BtsI EaeI EarI Eco57MI EcoO109I FalI Hin4I
HincII HindIII MscI MslI MspA1I NcoI NspI PflMI
PpuMI PstI PvuII SbfI SfcI SmlI SpeI SphI
StuI StyI TaqII TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BanII BbeI BbvCI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI
TstI Tth111I XhoI XmaI XmnI ZraI