Restriction map for
pFN21AE1959
(Linear) (Six-base) MAPSORT of: KIEE1959.seq Check: 7833 from: 1 to: 1548
KIEE1959.orf
With 182 enzymes: SgfI *
July 3, 2012 17:05 ..
AatII G_ACGT'C
Cuts at: 0 224 1548
Size: 224 1324
AccI GT'mk_AC
Cuts at: 0 1466 1548
Size: 1466 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1548
Size: 357 1191
AfeI AGC'GCT
Cuts at: 0 211 1548
Size: 211 1337
AflIII A'CryG_T
Cuts at: 0 1515 1548
Size: 1515 33
AgeI A'CCGG_T
Cuts at: 0 390 1548
Size: 390 1158
AleI CACnn'nnGTG
Cuts at: 0 250 1548
Size: 250 1298
AlwNI CAG_nnn'CTG
Cuts at: 0 291 385 534 547 1434 1548
Size: 291 94 149 13 887 114
Fragments arranged by size:
887 291 149 114 94 13
AvaI C'yCGr_G
Cuts at: 0 98 151 1548
Size: 98 53 1397
Fragments arranged by size:
1397 98 53
BanI G'GyrC_C
Cuts at: 0 410 601 853 1548
Size: 410 191 252 695
Fragments arranged by size:
695 410 252 191
BbeI G_GCGC'C
Cuts at: 0 414 1548
Size: 414 1134
BbsI GAAGACnn'nnnn_
Cuts at: 0 1212 1548
Size: 1212 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1328 1548
Size: 1294 34 220
Fragments arranged by size:
1294 220 34
BclI T'GATC_A
Cuts at: 0 515 1548
Size: 515 1033
BglII A'GATC_T
Cuts at: 0 1400 1548
Size: 1400 148
BlpI GC'TnA_GC
Cuts at: 0 262 649 1548
Size: 262 387 899
Fragments arranged by size:
899 387 262
Bme1580I G_kGCm'C
Cuts at: 0 554 858 1548
Size: 554 304 690
Fragments arranged by size:
690 554 304
BmrI ACTGGGnnnn_n'
Cuts at: 0 6 1548
Size: 6 1542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 705 1287 1548
Size: 705 582 261
Fragments arranged by size:
705 582 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1002 1138 1456 1548
Size: 502 500 136 318 92
Fragments arranged by size:
502 500 318 136 92
Bpu10I CC'TnA_GC
Cuts at: 0 506 1286 1548
Size: 506 780 262
Fragments arranged by size:
780 506 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1350 1548
Size: 67 1283 198
Fragments arranged by size:
1283 198 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 1114 1548
Size: 1114 434
BsaHI Gr'CG_yC
Cuts at: 0 221 411 1548
Size: 221 190 1137
Fragments arranged by size:
1137 221 190
BsaWI w'CCGG_w
Cuts at: 0 390 1548
Size: 390 1158
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 1548
Size: 424 30 1094
Fragments arranged by size:
1094 424 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 681 1125 1548
Size: 499 182 444 423
Fragments arranged by size:
499 444 423 182
BseYI C'CCAG_C
Cuts at: 0 456 1088 1548
Size: 456 632 460
Fragments arranged by size:
632 460 456
BsiHKAI G_wGCw'C
Cuts at: 0 83 812 1548
Size: 83 729 736
Fragments arranged by size:
736 729 83
BsmI GAATG_Cn'
Cuts at: 0 429 1548
Size: 429 1119
Bsp1286I G_dGCh'C
Cuts at: 0 83 554 812 858 1548
Size: 83 471 258 46 690
Fragments arranged by size:
690 471 258 83 46
BspHI T'CATG_A
Cuts at: 0 669 1548
Size: 669 879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 740 1227 1548
Size: 512 228 487 321
Fragments arranged by size:
512 487 321 228
BsrBI CCG'CTC
Cuts at: 0 1062 1290 1548
Size: 1062 228 258
Fragments arranged by size:
1062 258 228
BsrFI r'CCGG_y
Cuts at: 0 320 390 407 1367 1548
Size: 320 70 17 960 181
Fragments arranged by size:
960 320 181 70 17
BssSI C'ACGA_G
Cuts at: 0 655 1548
Size: 655 893
BstXI CCAn_nnnn'nTGG
Cuts at: 0 134 140 1259 1548
Size: 134 6 1119 289
Fragments arranged by size:
1119 289 134 6
BstYI r'GATC_y
Cuts at: 0 230 1025 1400 1548
Size: 230 795 375 148
Fragments arranged by size:
795 375 230 148
BtgI C'CryG_G
Cuts at: 0 133 1548
Size: 133 1415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 429 653 1548
Size: 429 224 895
Fragments arranged by size:
895 429 224
BtsI GCAGTG_nn'
Cuts at: 0 1310 1548
Size: 1310 238
EaeI y'GGCC_r
Cuts at: 0 29 357 587 1388 1415 1548
Size: 29 328 230 801 27 133
Fragments arranged by size:
801 328 230 133 29 27
EarI CTCTTCn'nnn_
Cuts at: 0 1414 1548
Size: 1414 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 502 1002 1138 1456 1548
Size: 357 145 500 136 318 92
Fragments arranged by size:
500 357 318 145 136 92
EcoO109I rG'GnC_Cy
Cuts at: 0 102 288 510 544 880 1266 1548
Size: 102 186 222 34 336 386 282
Fragments arranged by size:
386 336 282 222 186 102 34
FseI GG_CCGG'CC
Cuts at: 0 324 1548
Size: 324 1224
HaeII r_GCGC'y
Cuts at: 0 213 414 1368 1548
Size: 213 201 954 180
Fragments arranged by size:
954 213 201 180
HincII GTy'rAC
Cuts at: 0 924 1242 1467 1548
Size: 924 318 225 81
Fragments arranged by size:
924 318 225 81
KasI G'GCGC_C
Cuts at: 0 410 1548
Size: 410 1138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1152 1548
Size: 1152 396
MscI TGG'CCA
Cuts at: 0 359 589 1390 1417 1548
Size: 359 230 801 27 131
Fragments arranged by size:
801 359 230 131 27
MslI CAynn'nnrTG
Cuts at: 0 250 1548
Size: 250 1298
MspA1I CmG'CkG
Cuts at: 0 893 1092 1508 1548
Size: 893 199 416 40
Fragments arranged by size:
893 416 199 40
NaeI GCC'GGC
Cuts at: 0 322 409 1548
Size: 322 87 1139
Fragments arranged by size:
1139 322 87
NarI GG'CG_CC
Cuts at: 0 411 1548
Size: 411 1137
NcoI C'CATG_G
Cuts at: 0 133 1548
Size: 133 1415
NgoMIV G'CCGG_C
Cuts at: 0 320 407 1548
Size: 320 87 1141
Fragments arranged by size:
1141 320 87
NspI r_CATG'y
Cuts at: 0 160 493 1519 1548
Size: 160 333 1026 29
Fragments arranged by size:
1026 333 160 29
PasI CC'CwG_GG
Cuts at: 0 168 1548
Size: 168 1380
PciI A'CATG_T
Cuts at: 0 1515 1548
Size: 1515 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 980 1548
Size: 980 568
PfoI T'CCnGG_A
Cuts at: 0 694 803 839 979 1115 1548
Size: 694 109 36 140 136 433
Fragments arranged by size:
694 433 140 136 109 36
PpuMI rG'GwC_Cy
Cuts at: 0 102 1266 1548
Size: 102 1164 282
Fragments arranged by size:
1164 282 102
PstI C_TGCA'G
Cuts at: 0 230 752 794 1548
Size: 230 522 42 754
Fragments arranged by size:
754 522 230 42
PvuII CAG'CTG
Cuts at: 0 1092 1548
Size: 1092 456
SalI G'TCGA_C
Cuts at: 0 1465 1548
Size: 1465 83
SanDI GG'GwC_CC
Cuts at: 0 102 1548
Size: 102 1446
SfcI C'TryA_G
Cuts at: 0 226 748 790 1426 1548
Size: 226 522 42 636 122
Fragments arranged by size:
636 522 226 122 42
SfoI GGC'GCC
Cuts at: 0 412 1548
Size: 412 1136
SgrAI Cr'CCGG_yG
Cuts at: 0 407 1367 1548
Size: 407 960 181
Fragments arranged by size:
960 407 181
SmlI C'TyrA_G
Cuts at: 0 82 1329 1548
Size: 82 1247 219
Fragments arranged by size:
1247 219 82
StuI AGG'CCT
Cuts at: 0 96 113 763 1108 1548
Size: 96 17 650 345 440
Fragments arranged by size:
650 440 345 96 17
StyI C'CwwG_G
Cuts at: 0 133 1548
Size: 133 1415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 66 1548
Size: 66 1482
Tth111I GACn'n_nGTC
Cuts at: 0 961 1548
Size: 961 587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 131 252 1548
Size: 63 68 121 1296
Fragments arranged by size:
1296 121 68 63
XmnI GAAnn'nnTTC
Cuts at: 0 1192 1548
Size: 1192 356
ZraI GAC'GTC
Cuts at: 0 222 1548
Size: 222 1326
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AgeI AleI AlwNI
AvaI BanI BbeI BbsI BcgI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoO109I FseI HaeII HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PstI
PvuII SalI SanDI SfcI SfoI SgrAI SmlI StuI
StyI TaqII Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AhdI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbvCI BciVI BfrBI BglI BmgBI BmtI BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI BspEI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FspI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SapI SbfI ScaI SexAI
SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI XbaI XhoI XmaI