Restriction map for
pFN21AE1958
(Linear) (Six-base) MAPSORT of: KIEE1958.seq Check: 1827 from: 1 to: 1542
KIEE1958.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1215 1542
Size: 1215 327
AccI GT'mk_AC
Cuts at: 0 411 1542
Size: 411 1131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 767 1542
Size: 607 160 775
Fragments arranged by size:
775 607 160
AfeI AGC'GCT
Cuts at: 0 810 1542
Size: 810 732
AflIII A'CryG_T
Cuts at: 0 209 1129 1542
Size: 209 920 413
Fragments arranged by size:
920 413 209
AlwNI CAG_nnn'CTG
Cuts at: 0 24 132 157 533 870 1542
Size: 24 108 25 376 337 672
Fragments arranged by size:
672 376 337 108 25 24
ApaI G_GGCC'C
Cuts at: 0 31 582 821 1542
Size: 31 551 239 721
Fragments arranged by size:
721 551 239 31
AvaI C'yCGr_G
Cuts at: 0 768 946 1049 1542
Size: 768 178 103 493
Fragments arranged by size:
768 493 178 103
AvrII C'CTAG_G
Cuts at: 0 1464 1542
Size: 1464 78
BamHI G'GATC_C
Cuts at: 0 1375 1542
Size: 1375 167
BanI G'GyrC_C
Cuts at: 0 83 367 1428 1469 1542
Size: 83 284 1061 41 73
Fragments arranged by size:
1061 284 83 73 41
BanII G_rGCy'C
Cuts at: 0 31 121 582 821 1238 1542
Size: 31 90 461 239 417 304
Fragments arranged by size:
461 417 304 239 90 31
BbeI G_GCGC'C
Cuts at: 0 87 371 1473 1542
Size: 87 284 1102 69
Fragments arranged by size:
1102 284 87 69
BbvCI CC'TCA_GC
Cuts at: 0 1072 1542
Size: 1072 470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 681 715 914 948 1542
Size: 681 34 199 34 594
Fragments arranged by size:
681 594 199 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 684 1542
Size: 684 858
BglI GCCn_nnn'nGGC
Cuts at: 0 493 577 1542
Size: 493 84 965
Fragments arranged by size:
965 493 84
BlpI GC'TnA_GC
Cuts at: 0 805 916 1542
Size: 805 111 626
Fragments arranged by size:
805 626 111
Bme1580I G_kGCm'C
Cuts at: 0 31 582 821 1542
Size: 31 551 239 721
Fragments arranged by size:
721 551 239 31
BmgBI CAC'GTC
Cuts at: 0 669 1542
Size: 669 873
BmrI ACTGGGnnnn_n'
Cuts at: 0 12 252 1542
Size: 12 240 1290
Fragments arranged by size:
1290 240 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1458 1542
Size: 1458 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 205 440 510 733 1542
Size: 50 155 235 70 223 809
Fragments arranged by size:
809 235 223 155 70 50
Bpu10I CC'TnA_GC
Cuts at: 0 139 1072 1542
Size: 139 933 470
Fragments arranged by size:
933 470 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1489 1542
Size: 767 722 53
Fragments arranged by size:
767 722 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 1542
Size: 486 1056
BsaHI Gr'CG_yC
Cuts at: 0 84 368 1470 1542
Size: 84 284 1102 72
Fragments arranged by size:
1102 284 84 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 869 899 1542
Size: 505 30 334 30 643
Fragments arranged by size:
643 505 334 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 510 513 612 934 1089 1542
Size: 510 3 99 322 155 453
Fragments arranged by size:
510 453 322 155 99 3
BseYI C'CCAG_C
Cuts at: 0 24 357 437 1184 1191 1205 1453 1542
Size: 24 333 80 747 7 14 248 89
Fragments arranged by size:
747 333 248 89 80 24 14 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1024 1542
Size: 907 117 518
Fragments arranged by size:
907 518 117
BsiEI CG_ry'CG
Cuts at: 0 617 701 1542
Size: 617 84 841
Fragments arranged by size:
841 617 84
BsiHKAI G_wGCw'C
Cuts at: 0 98 121 694 1081 1542
Size: 98 23 573 387 461
Fragments arranged by size:
573 461 387 98 23
Bsp1286I G_dGCh'C
Cuts at: 0 31 98 121 582 694 821 1081 1238
Size: 31 67 23 461 112 127 260 157
Cuts at: 1238 1542
Size: 304
Fragments arranged by size:
461 304 260 157 127 112 67 31
23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 423 1215 1542
Size: 423 792 327
Fragments arranged by size:
792 423 327
BsrBI CCG'CTC
Cuts at: 0 756 1542
Size: 756 786
BsrDI GCAATG_nn'
Cuts at: 0 790 1542
Size: 790 752
BsrFI r'CCGG_y
Cuts at: 0 559 1109 1542
Size: 559 550 433
Fragments arranged by size:
559 550 433
BstEII G'GTnAC_C
Cuts at: 0 1105 1542
Size: 1105 437
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1300 1542
Size: 1300 242
BstYI r'GATC_y
Cuts at: 0 536 1375 1542
Size: 536 839 167
Fragments arranged by size:
839 536 167
BstZ17I GTA'TAC
Cuts at: 0 412 1542
Size: 412 1130
BtsI GCAGTG_nn'
Cuts at: 0 1120 1195 1413 1542
Size: 1120 75 218 129
Fragments arranged by size:
1120 218 129 75
EaeI y'GGCC_r
Cuts at: 0 2 267 569 1290 1542
Size: 2 265 302 721 252
Fragments arranged by size:
721 302 265 252 2
EarI CTCTTCn'nnn_
Cuts at: 0 1397 1542
Size: 1397 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 615 859 1450 1542
Size: 615 244 591 92
Fragments arranged by size:
615 591 244 92
EcoICRI GAG'CTC
Cuts at: 0 119 1542
Size: 119 1423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 205 440 510 607 733 767 1542
Size: 50 155 235 70 97 126 34 775
Fragments arranged by size:
775 235 155 126 97 70 50 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 419 1542
Size: 419 1123
EcoO109I rG'GnC_Cy
Cuts at: 0 28 129 707 818 1439 1542
Size: 28 101 578 111 621 103
Fragments arranged by size:
621 578 111 103 101 28
HaeII r_GCGC'y
Cuts at: 0 87 371 812 862 913 1473 1542
Size: 87 284 441 50 51 560 69
Fragments arranged by size:
560 441 284 87 69 51 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 324 356 466 498 658 690
Size: 208 32 84 32 110 32 160 32
Cuts at: 690 929 961 1542
Size: 239 32 581
Fragments arranged by size:
581 239 208 160 110 84 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1041 1542
Size: 1041 501
KasI G'GCGC_C
Cuts at: 0 83 367 1469 1542
Size: 83 284 1102 73
Fragments arranged by size:
1102 284 83 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1542
Size: 239 1303
MscI TGG'CCA
Cuts at: 0 269 1292 1542
Size: 269 1023 250
Fragments arranged by size:
1023 269 250
MspA1I CmG'CkG
Cuts at: 0 1071 1542
Size: 1071 471
NaeI GCC'GGC
Cuts at: 0 561 1542
Size: 561 981
NarI GG'CG_CC
Cuts at: 0 84 368 1470 1542
Size: 84 284 1102 72
Fragments arranged by size:
1102 284 84 72
NgoMIV G'CCGG_C
Cuts at: 0 559 1542
Size: 559 983
NspI r_CATG'y
Cuts at: 0 1133 1351 1542
Size: 1133 218 191
Fragments arranged by size:
1133 218 191
PasI CC'CwG_GG
Cuts at: 0 126 822 1300 1542
Size: 126 696 478 242
Fragments arranged by size:
696 478 242 126
PciI A'CATG_T
Cuts at: 0 1129 1542
Size: 1129 413
PflMI CCAn_nnn'nTGG
Cuts at: 0 24 132 533 1263 1419 1542
Size: 24 108 401 730 156 123
Fragments arranged by size:
730 401 156 123 108 24
PpuMI rG'GwC_Cy
Cuts at: 0 707 1542
Size: 707 835
PspOMI G'GGCC_C
Cuts at: 0 27 578 817 1542
Size: 27 551 239 725
Fragments arranged by size:
725 551 239 27
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1267 1299 1542
Size: 1267 32 243
Fragments arranged by size:
1267 243 32
PstI C_TGCA'G
Cuts at: 0 162 866 1542
Size: 162 704 676
Fragments arranged by size:
704 676 162
PvuI CG_AT'CG
Cuts at: 0 701 1542
Size: 701 841
PvuII CAG'CTG
Cuts at: 0 1071 1542
Size: 1071 471
SacI G_AGCT'C
Cuts at: 0 121 1542
Size: 121 1421
SanDI GG'GwC_CC
Cuts at: 0 707 1542
Size: 707 835
SfcI C'TryA_G
Cuts at: 0 158 232 862 1492 1542
Size: 158 74 630 630 50
Fragments arranged by size:
630 630 158 74 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 577 1542
Size: 577 965
SfoI GGC'GCC
Cuts at: 0 85 369 1471 1542
Size: 85 284 1102 71
Fragments arranged by size:
1102 284 85 71
SmlI C'TyrA_G
Cuts at: 0 782 1504 1542
Size: 782 722 38
Fragments arranged by size:
782 722 38
StuI AGG'CCT
Cuts at: 0 425 1542
Size: 425 1117
StyI C'CwwG_G
Cuts at: 0 312 350 566 1464 1542
Size: 312 38 216 898 78
Fragments arranged by size:
898 312 216 78 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 234 603 1093 1542
Size: 79 155 369 490 449
Fragments arranged by size:
490 449 369 155 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 869 901 1542
Size: 869 32 641
Fragments arranged by size:
869 641 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 176 262 1542
Size: 176 86 1280
Fragments arranged by size:
1280 176 86
XmnI GAAnn'nnTTC
Cuts at: 0 1480 1542
Size: 1480 62
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII BstXI
BstYI BstZ17I BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII KasI MmeI MscI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PpuMI PspOMI PsrI PstI PvuI PvuII SacI SanDI
SfcI SfiI SfoI SmlI StuI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BbsI
BclI BfrBI BglII BmtI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI
BstBI Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MslI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI RsrII SacII SalI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI