Restriction map for
pFN21AE1956
(Linear) (Six-base) MAPSORT of: KIEE1956.seq Check: 3549 from: 1 to: 1539
KIEE1956.orf
With 182 enzymes: SgfI *
March 22, 2012 15:55 ..
AccI GT'mk_AC
Cuts at: 0 551 1539
Size: 551 988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1539
Size: 1139 400
AflIII A'CryG_T
Cuts at: 0 379 1539
Size: 379 1160
AhdI GACnn_n'nnGTC
Cuts at: 0 645 1539
Size: 645 894
AlwNI CAG_nnn'CTG
Cuts at: 0 435 519 999 1539
Size: 435 84 480 540
Fragments arranged by size:
540 480 435 84
ApaI G_GGCC'C
Cuts at: 0 1321 1539
Size: 1321 218
ApoI r'AATT_y
Cuts at: 0 968 1539
Size: 968 571
AvaI C'yCGr_G
Cuts at: 0 66 269 1539
Size: 66 203 1270
Fragments arranged by size:
1270 203 66
BamHI G'GATC_C
Cuts at: 0 93 825 1265 1539
Size: 93 732 440 274
Fragments arranged by size:
732 440 274 93
BanI G'GyrC_C
Cuts at: 0 53 1539
Size: 53 1486
BanII G_rGCy'C
Cuts at: 0 1321 1521 1539
Size: 1321 200 18
Fragments arranged by size:
1321 200 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 508 1539
Size: 508 1031
BclI T'GATC_A
Cuts at: 0 617 1539
Size: 617 922
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1539
Size: 34 1505
Bme1580I G_kGCm'C
Cuts at: 0 121 1321 1539
Size: 121 1200 218
Fragments arranged by size:
1200 218 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 450 464 507 1539
Size: 417 33 14 43 1032
Fragments arranged by size:
1032 417 43 33 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 1447 1539
Size: 1447 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 813 843 1539
Size: 813 30 696
Fragments arranged by size:
813 696 30
BsiHKAI G_wGCw'C
Cuts at: 0 1187 1539
Size: 1187 352
Bsp1286I G_dGCh'C
Cuts at: 0 121 1187 1321 1521 1539
Size: 121 1066 134 200 18
Fragments arranged by size:
1066 200 134 121 18
BsrDI GCAATG_nn'
Cuts at: 0 1227 1379 1539
Size: 1227 152 160
Fragments arranged by size:
1227 160 152
BstEII G'GTnAC_C
Cuts at: 0 150 1539
Size: 150 1389
BstXI CCAn_nnnn'nTGG
Cuts at: 0 407 1539
Size: 407 1132
BstYI r'GATC_y
Cuts at: 0 93 825 1265 1539
Size: 93 732 440 274
Fragments arranged by size:
732 440 274 93
BtgI C'CryG_G
Cuts at: 0 29 1539
Size: 29 1510
BtsI GCAGTG_nn'
Cuts at: 0 322 1539
Size: 322 1217
EarI CTCTTCn'nnn_
Cuts at: 0 140 573 1539
Size: 140 433 966
Fragments arranged by size:
966 433 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1082 1539
Size: 1082 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1539
Size: 1139 400
EcoO109I rG'GnC_Cy
Cuts at: 0 1317 1318 1539
Size: 1317 1 221
Fragments arranged by size:
1317 221 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 631 663 813 845 1539
Size: 631 32 150 32 694
Fragments arranged by size:
694 631 150 32 32
HincII GTy'rAC
Cuts at: 0 475 626 1150 1539
Size: 475 151 524 389
Fragments arranged by size:
524 475 389 151
HindIII A'AGCT_T
Cuts at: 0 987 1539
Size: 987 552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1539
Size: 573 966
MslI CAynn'nnrTG
Cuts at: 0 817 1310 1539
Size: 817 493 229
Fragments arranged by size:
817 493 229
MspA1I CmG'CkG
Cuts at: 0 114 1539
Size: 114 1425
NspI r_CATG'y
Cuts at: 0 383 1539
Size: 383 1156
PciI A'CATG_T
Cuts at: 0 379 1539
Size: 379 1160
PflMI CCAn_nnn'nTGG
Cuts at: 0 938 999 1539
Size: 938 61 540
Fragments arranged by size:
938 540 61
PfoI T'CCnGG_A
Cuts at: 0 10 1261 1539
Size: 10 1251 278
Fragments arranged by size:
1251 278 10
PsiI TTA'TAA
Cuts at: 0 725 1037 1539
Size: 725 312 502
Fragments arranged by size:
725 502 312
PspOMI G'GGCC_C
Cuts at: 0 1317 1539
Size: 1317 222
ScaI AGT'ACT
Cuts at: 0 584 1368 1539
Size: 584 784 171
Fragments arranged by size:
784 584 171
SexAI A'CCwGG_T
Cuts at: 0 1007 1539
Size: 1007 532
SfcI C'TryA_G
Cuts at: 0 198 1272 1539
Size: 198 1074 267
Fragments arranged by size:
1074 267 198
StyI C'CwwG_G
Cuts at: 0 156 601 1205 1539
Size: 156 445 604 334
Fragments arranged by size:
604 445 334 156
TatI w'GTAC_w
Cuts at: 0 582 1366 1539
Size: 582 784 173
Fragments arranged by size:
784 582 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 1074 1106 1539
Size: 62 32 980 32 433
Fragments arranged by size:
980 433 62 32 32
XbaI T'CTAG_A
Cuts at: 0 1164 1539
Size: 1164 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1528 1539
Size: 1528 11
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BclI BglI Bme1580I BmrI
BsaI BsaXI BsiHKAI Bsp1286I BsrDI BstEII BstXI BstYI
BtgI BtsI EarI EciI Eco57MI EcoO109I Hin4I HincII
HindIII MmeI MslI MspA1I NspI PciI PflMI PfoI
PsiI PspOMI ScaI SexAI SfcI StyI TatI TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XhoI XmaI
XmnI ZraI