Restriction map for
pFN21AE1955
(Linear) (Six-base) MAPSORT of: KIEE1955.seq Check: 7774 from: 1 to: 1539
KIEE1955.orf
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 964 1184 1539
Size: 406 558 220 355
Fragments arranged by size:
558 406 355 220
AhdI GACnn_n'nnGTC
Cuts at: 0 453 1539
Size: 453 1086
AlwNI CAG_nnn'CTG
Cuts at: 0 30 62 129 354 1539
Size: 30 32 67 225 1185
Fragments arranged by size:
1185 225 67 32 30
ApaI G_GGCC'C
Cuts at: 0 71 1539
Size: 71 1468
ApaLI G'TGCA_C
Cuts at: 0 1102 1539
Size: 1102 437
ApoI r'AATT_y
Cuts at: 0 763 919 1539
Size: 763 156 620
Fragments arranged by size:
763 620 156
AvaI C'yCGr_G
Cuts at: 0 189 376 525 1022 1408 1539
Size: 189 187 149 497 386 131
Fragments arranged by size:
497 386 189 187 149 131
BanI G'GyrC_C
Cuts at: 0 529 1136 1539
Size: 529 607 403
Fragments arranged by size:
607 529 403
BanII G_rGCy'C
Cuts at: 0 71 148 266 371 1539
Size: 71 77 118 105 1168
Fragments arranged by size:
1168 118 105 77 71
BbeI G_GCGC'C
Cuts at: 0 533 1140 1539
Size: 533 607 399
Fragments arranged by size:
607 533 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 374 482 516 1539
Size: 340 34 108 34 1023
Fragments arranged by size:
1023 340 108 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1040 1539
Size: 1040 499
BglII A'GATC_T
Cuts at: 0 245 1539
Size: 245 1294
BlpI GC'TnA_GC
Cuts at: 0 985 1539
Size: 985 554
Bme1580I G_kGCm'C
Cuts at: 0 71 1106 1539
Size: 71 1035 433
Fragments arranged by size:
1035 433 71
BmgBI CAC'GTC
Cuts at: 0 1014 1179 1539
Size: 1014 165 360
Fragments arranged by size:
1014 360 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 1161 1539
Size: 1161 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1176 1539
Size: 1113 63 363
Fragments arranged by size:
1113 363 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1539
Size: 196 1343
BsaI GGTCTCn'nnnn_
Cuts at: 0 1194 1539
Size: 1194 345
BsaAI yAC'GTr
Cuts at: 0 1107 1539
Size: 1107 432
BsaHI Gr'CG_yC
Cuts at: 0 454 530 1137 1539
Size: 454 76 607 402
Fragments arranged by size:
607 454 402 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 998 1028 1539
Size: 235 30 733 30 511
Fragments arranged by size:
733 511 235 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 346 1539
Size: 177 169 1193
Fragments arranged by size:
1193 177 169
BseYI C'CCAG_C
Cuts at: 0 9 129 258 506 1539
Size: 9 120 129 248 1033
Fragments arranged by size:
1033 248 129 120 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 271 1402 1539
Size: 75 196 1131 137
Fragments arranged by size:
1131 196 137 75
BsiEI CG_ry'CG
Cuts at: 0 107 153 1539
Size: 107 46 1386
Fragments arranged by size:
1386 107 46
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1539
Size: 1106 433
BsmI GAATG_Cn'
Cuts at: 0 1336 1365 1539
Size: 1336 29 174
Fragments arranged by size:
1336 174 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1040 1539
Size: 1040 499
Bsp1286I G_dGCh'C
Cuts at: 0 71 148 266 371 1106 1539
Size: 71 77 118 105 735 433
Fragments arranged by size:
735 433 118 105 77 71
BspHI T'CATG_A
Cuts at: 0 644 1539
Size: 644 895
BsrBI CCG'CTC
Cuts at: 0 543 1539
Size: 543 996
BsrDI GCAATG_nn'
Cuts at: 0 1289 1539
Size: 1289 250
BsrFI r'CCGG_y
Cuts at: 0 203 272 1133 1539
Size: 203 69 861 406
Fragments arranged by size:
861 406 203 69
BsrGI T'GTAC_A
Cuts at: 0 416 1539
Size: 416 1123
BssHII G'CGCG_C
Cuts at: 0 605 1539
Size: 605 934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 62 354 1539
Size: 62 292 1185
Fragments arranged by size:
1185 292 62
BstYI r'GATC_y
Cuts at: 0 245 411 571 1539
Size: 245 166 160 968
Fragments arranged by size:
968 245 166 160
Bsu36I CC'TnA_GG
Cuts at: 0 737 1539
Size: 737 802
BtgI C'CryG_G
Cuts at: 0 101 1539
Size: 101 1438
BtsI GCAGTG_nn'
Cuts at: 0 31 1221 1539
Size: 31 1190 318
Fragments arranged by size:
1190 318 31
DraI TTT'AAA
Cuts at: 0 801 1539
Size: 801 738
DrdI GACnn_nn'nnGTC
Cuts at: 0 1148 1539
Size: 1148 391
EaeI y'GGCC_r
Cuts at: 0 104 150 932 1539
Size: 104 46 782 607
Fragments arranged by size:
782 607 104 46
EagI C'GGCC_G
Cuts at: 0 104 150 1539
Size: 104 46 1389
Fragments arranged by size:
1389 104 46
EarI CTCTTCn'nnn_
Cuts at: 0 406 1539
Size: 406 1133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 420 1539
Size: 30 390 1119
Fragments arranged by size:
1119 390 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 964 1113 1176 1184 1539
Size: 406 558 149 63 8 355
Fragments arranged by size:
558 406 355 149 63 8
EcoO109I rG'GnC_Cy
Cuts at: 0 68 1539
Size: 68 1471
EcoRI G'AATT_C
Cuts at: 0 763 1539
Size: 763 776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 738 1086 1539
Size: 264 474 348 453
Fragments arranged by size:
474 453 348 264
FspI TGC'GCA
Cuts at: 0 1111 1539
Size: 1111 428
FspAI rTGC'GCAy
Cuts at: 0 1111 1539
Size: 1111 428
HaeII r_GCGC'y
Cuts at: 0 319 533 620 1140 1539
Size: 319 214 87 520 399
Fragments arranged by size:
520 399 319 214 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 439 471 998 1030 1140 1172
Size: 235 32 172 32 527 32 110 32
Cuts at: 1172 1465 1497 1539
Size: 293 32 42
Fragments arranged by size:
527 293 235 172 110 42 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 529 1136 1539
Size: 529 607 403
Fragments arranged by size:
607 529 403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 437 857 1539
Size: 321 116 420 682
Fragments arranged by size:
682 420 321 116
MslI CAynn'nnrTG
Cuts at: 0 643 1358 1539
Size: 643 715 181
Fragments arranged by size:
715 643 181
MspA1I CmG'CkG
Cuts at: 0 59 78 97 103 126 354 581 984
Size: 59 19 19 6 23 228 227 403
Cuts at: 984 1539
Size: 555
Fragments arranged by size:
555 403 228 227 59 23 19 19
6
NaeI GCC'GGC
Cuts at: 0 1135 1539
Size: 1135 404
NarI GG'CG_CC
Cuts at: 0 530 1137 1539
Size: 530 607 402
Fragments arranged by size:
607 530 402
NgoMIV G'CCGG_C
Cuts at: 0 1133 1539
Size: 1133 406
NotI GC'GGCC_GC
Cuts at: 0 104 1539
Size: 104 1435
PflMI CCAn_nnn'nTGG
Cuts at: 0 1179 1269 1539
Size: 1179 90 270
Fragments arranged by size:
1179 270 90
PmlI CAC'GTG
Cuts at: 0 1107 1539
Size: 1107 432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 1539
Size: 700 32 807
Fragments arranged by size:
807 700 32
PshAI GACnn'nnGTC
Cuts at: 0 387 1539
Size: 387 1152
PspOMI G'GGCC_C
Cuts at: 0 67 1539
Size: 67 1472
PstI C_TGCA'G
Cuts at: 0 83 762 1237 1539
Size: 83 679 475 302
Fragments arranged by size:
679 475 302 83
PvuII CAG'CTG
Cuts at: 0 59 126 354 984 1539
Size: 59 67 228 630 555
Fragments arranged by size:
630 555 228 67 59
SacII CC_GC'GG
Cuts at: 0 104 1539
Size: 104 1435
SbfI CC_TGCA'GG
Cuts at: 0 1237 1539
Size: 1237 302
SexAI A'CCwGG_T
Cuts at: 0 659 1117 1539
Size: 659 458 422
Fragments arranged by size:
659 458 422
SfcI C'TryA_G
Cuts at: 0 79 758 1233 1539
Size: 79 679 475 306
Fragments arranged by size:
679 475 306 79
SfoI GGC'GCC
Cuts at: 0 531 1138 1539
Size: 531 607 401
Fragments arranged by size:
607 531 401
SmaI CCC'GGG
Cuts at: 0 378 527 1539
Size: 378 149 1012
Fragments arranged by size:
1012 378 149
SmlI C'TyrA_G
Cuts at: 0 211 1539
Size: 211 1328
StuI AGG'CCT
Cuts at: 0 1034 1539
Size: 1034 505
StyI C'CwwG_G
Cuts at: 0 1298 1388 1539
Size: 1298 90 151
Fragments arranged by size:
1298 151 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1311 1539
Size: 1311 228
TatI w'GTAC_w
Cuts at: 0 416 1539
Size: 416 1123
Tth111I GACn'n_nGTC
Cuts at: 0 640 1539
Size: 640 899
XmaI C'CCGG_G
Cuts at: 0 376 525 1539
Size: 376 149 1014
Fragments arranged by size:
1014 376 149
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I
BtgI BtsI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
KasI MmeI MslI MspA1I NaeI NarI NgoMIV NotI
PflMI PmlI PpiI PshAI PspOMI PstI PvuII SacII
SbfI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BmtI
BplI Bpu10I BsaBI BsaWI BsiWI BspEI BspMI BssSI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII
EcoICRI EcoNI EcoRV FseI HincII HindIII HpaI KpnI
MfeI MluI MscI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PpuMI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XcmI XhoI XmnI ZraI