Restriction map for
pFN21AE1946
(Linear) (Six-base) MAPSORT of: KIEE1946.seq Check: 5864 from: 1 to: 1503
KIEE1946.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1399 1503
Size: 1399 104
Acc65I G'GTAC_C
Cuts at: 0 35 1503
Size: 35 1468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 457 808 1503
Size: 156 301 351 695
Fragments arranged by size:
695 351 301 156
AflII C'TTAA_G
Cuts at: 0 1186 1503
Size: 1186 317
AflIII A'CryG_T
Cuts at: 0 665 1503
Size: 665 838
AhdI GACnn_n'nnGTC
Cuts at: 0 1374 1503
Size: 1374 129
AlwNI CAG_nnn'CTG
Cuts at: 0 415 1353 1503
Size: 415 938 150
Fragments arranged by size:
938 415 150
ApaI G_GGCC'C
Cuts at: 0 461 1146 1503
Size: 461 685 357
Fragments arranged by size:
685 461 357
ApoI r'AATT_y
Cuts at: 0 58 1503
Size: 58 1445
AvaI C'yCGr_G
Cuts at: 0 454 1372 1503
Size: 454 918 131
Fragments arranged by size:
918 454 131
BanI G'GyrC_C
Cuts at: 0 35 79 235 396 1503
Size: 35 44 156 161 1107
Fragments arranged by size:
1107 161 156 44 35
BanII G_rGCy'C
Cuts at: 0 455 461 473 1146 1328 1503
Size: 455 6 12 673 182 175
Fragments arranged by size:
673 455 182 175 12 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 162 1503
Size: 162 1341
BglI GCCn_nnn'nGGC
Cuts at: 0 809 1150 1407 1503
Size: 809 341 257 96
Fragments arranged by size:
809 341 257 96
Bme1580I G_kGCm'C
Cuts at: 0 84 461 684 1146 1403 1503
Size: 84 377 223 462 257 100
Fragments arranged by size:
462 377 257 223 100 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 952 958 1342 1503
Size: 706 246 6 384 161
Fragments arranged by size:
706 384 246 161 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1503
Size: 1285 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 93 1503
Size: 93 1410
BsaAI yAC'GTr
Cuts at: 0 666 1503
Size: 666 837
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 1503
Size: 7 1496
BseYI C'CCAG_C
Cuts at: 0 421 702 782 805 869 920 1174 1238
Size: 421 281 80 23 64 51 254 64
Cuts at: 1238 1503
Size: 265
Fragments arranged by size:
421 281 265 254 80 64 64 51
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1503
Size: 51 1452
BsiEI CG_ry'CG
Cuts at: 0 813 990 1503
Size: 813 177 513
Fragments arranged by size:
813 513 177
Bsp1286I G_dGCh'C
Cuts at: 0 84 455 461 473 684 1146 1328 1403
Size: 84 371 6 12 211 462 182 75
Cuts at: 1403 1503
Size: 100
Fragments arranged by size:
462 371 211 182 100 84 75 12
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 993 1399 1503
Size: 993 406 104
Fragments arranged by size:
993 406 104
BsrBI CCG'CTC
Cuts at: 0 1082 1503
Size: 1082 421
BsrDI GCAATG_nn'
Cuts at: 0 1306 1503
Size: 1306 197
BsrFI r'CCGG_y
Cuts at: 0 812 819 1503
Size: 812 7 684
Fragments arranged by size:
812 684 7
BsrGI T'GTAC_A
Cuts at: 0 527 1503
Size: 527 976
BssSI C'ACGA_G
Cuts at: 0 464 508 1009 1503
Size: 464 44 501 494
Fragments arranged by size:
501 494 464 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1452 1503
Size: 1452 51
BstYI r'GATC_y
Cuts at: 0 412 719 862 1503
Size: 412 307 143 641
Fragments arranged by size:
641 412 307 143
BtgI C'CryG_G
Cuts at: 0 644 962 972 1364 1503
Size: 644 318 10 392 139
Fragments arranged by size:
644 392 318 139 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1239 1503
Size: 1239 264
BtsI GCAGTG_nn'
Cuts at: 0 1210 1281 1503
Size: 1210 71 222
Fragments arranged by size:
1210 222 71
EaeI y'GGCC_r
Cuts at: 0 125 593 746 810 1035 1086 1151 1503
Size: 125 468 153 64 225 51 65 352
Fragments arranged by size:
468 352 225 153 125 65 64 51
EagI C'GGCC_G
Cuts at: 0 810 1503
Size: 810 693
EarI CTCTTCn'nnn_
Cuts at: 0 901 1435 1503
Size: 901 534 68
Fragments arranged by size:
901 534 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 457 706 808 952 958 1342 1503
Size: 156 301 249 102 144 6 384 161
Fragments arranged by size:
384 301 249 161 156 144 102 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 1503
Size: 688 815
EcoO109I rG'GnC_Cy
Cuts at: 0 149 458 640 1017 1053 1143 1503
Size: 149 309 182 377 36 90 360
Fragments arranged by size:
377 360 309 182 149 90 36
EcoRI G'AATT_C
Cuts at: 0 58 1503
Size: 58 1445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 397 1503
Size: 397 1106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 826 858 1360 1392 1503
Size: 826 32 502 32 111
Fragments arranged by size:
826 502 111 32 32
HincII GTy'rAC
Cuts at: 0 1221 1503
Size: 1221 282
KpnI G_GTAC'C
Cuts at: 0 39 1503
Size: 39 1464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1183 1503
Size: 560 623 320
Fragments arranged by size:
623 560 320
MscI TGG'CCA
Cuts at: 0 127 595 1088 1153 1503
Size: 127 468 493 65 350
Fragments arranged by size:
493 468 350 127 65
MslI CAynn'nnrTG
Cuts at: 0 191 1480 1503
Size: 191 1289 23
Fragments arranged by size:
1289 191 23
MspA1I CmG'CkG
Cuts at: 0 757 786 800 809 924 1128 1503
Size: 757 29 14 9 115 204 375
Fragments arranged by size:
757 375 204 115 29 14 9
NaeI GCC'GGC
Cuts at: 0 814 821 1503
Size: 814 7 682
Fragments arranged by size:
814 682 7
NcoI C'CATG_G
Cuts at: 0 1364 1503
Size: 1364 139
NgoMIV G'CCGG_C
Cuts at: 0 812 819 1503
Size: 812 7 684
Fragments arranged by size:
812 684 7
NspI r_CATG'y
Cuts at: 0 519 1219 1503
Size: 519 700 284
Fragments arranged by size:
700 519 284
PasI CC'CwG_GG
Cuts at: 0 105 1503
Size: 105 1398
PfoI T'CCnGG_A
Cuts at: 0 384 1503
Size: 384 1119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1503
Size: 1102 32 369
Fragments arranged by size:
1102 369 32
PpuMI rG'GwC_Cy
Cuts at: 0 640 1053 1503
Size: 640 413 450
Fragments arranged by size:
640 450 413
PshAI GACnn'nnGTC
Cuts at: 0 638 1503
Size: 638 865
PspOMI G'GGCC_C
Cuts at: 0 457 1142 1503
Size: 457 685 361
Fragments arranged by size:
685 457 361
PstI C_TGCA'G
Cuts at: 0 1201 1501 1503
Size: 1201 300 2
Fragments arranged by size:
1201 300 2
PvuII CAG'CTG
Cuts at: 0 786 800 924 1503
Size: 786 14 124 579
Fragments arranged by size:
786 579 124 14
SapI GCTCTTCn'nnn_
Cuts at: 0 901 1503
Size: 901 602
SfcI C'TryA_G
Cuts at: 0 350 1197 1497 1503
Size: 350 847 300 6
Fragments arranged by size:
847 350 300 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1150 1503
Size: 1150 353
SmaI CCC'GGG
Cuts at: 0 456 1503
Size: 456 1047
SmlI C'TyrA_G
Cuts at: 0 1186 1264 1503
Size: 1186 78 239
Fragments arranged by size:
1186 239 78
SrfI GCCC'GGGC
Cuts at: 0 456 1503
Size: 456 1047
StuI AGG'CCT
Cuts at: 0 254 854 943 1503
Size: 254 600 89 560
Fragments arranged by size:
600 560 254 89
StyI C'CwwG_G
Cuts at: 0 140 473 1364 1503
Size: 140 333 891 139
Fragments arranged by size:
891 333 140 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 650 1401 1503
Size: 67 583 751 102
Fragments arranged by size:
751 583 102 67
TatI w'GTAC_w
Cuts at: 0 512 527 1503
Size: 512 15 976
Fragments arranged by size:
976 512 15
Tth111I GACn'n_nGTC
Cuts at: 0 537 1477 1503
Size: 537 940 26
Fragments arranged by size:
940 537 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 1503
Size: 120 1383
XmaI C'CCGG_G
Cuts at: 0 454 1503
Size: 454 1049
XmnI GAAnn'nnTTC
Cuts at: 0 169 895 1503
Size: 169 726 608
Fragments arranged by size:
726 608 169
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AhdI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BglI Bme1580I BpmI
BpuEI BsaI BsaAI BseRI BseYI BsgI BsiEI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SapI SfcI
SfiI SmaI SmlI SrfI StuI StyI TaqII TatI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI TstI XbaI XhoI ZraI