Restriction map for
pFN21AE1945
(Linear) (Six-base) MAPSORT of: KIEE1945.seq Check: 5779 from: 1 to: 1500
KIEE1945.orf
With 182 enzymes: SgfI *
July 3, 2012 17:04 ..
AccI GT'mk_AC
Cuts at: 0 421 1500
Size: 421 1079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1300 1500
Size: 929 371 200
Fragments arranged by size:
929 371 200
AflIII A'CryG_T
Cuts at: 0 686 1500
Size: 686 814
AleI CACnn'nnGTG
Cuts at: 0 1015 1500
Size: 1015 485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1033 1500
Size: 1033 467
AlwNI CAG_nnn'CTG
Cuts at: 0 473 1500
Size: 473 1027
ApaI G_GGCC'C
Cuts at: 0 750 828 1500
Size: 750 78 672
Fragments arranged by size:
750 672 78
ApaLI G'TGCA_C
Cuts at: 0 1378 1500
Size: 1378 122
ApoI r'AATT_y
Cuts at: 0 1120 1500
Size: 1120 380
AvaI C'yCGr_G
Cuts at: 0 937 1500
Size: 937 563
BanII G_rGCy'C
Cuts at: 0 398 750 828 938 1171 1500
Size: 398 352 78 110 233 329
Fragments arranged by size:
398 352 329 233 110 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 600 1500
Size: 600 900
BbvCI CC'TCA_GC
Cuts at: 0 1432 1500
Size: 1432 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 261 271 295 305 1500
Size: 261 10 24 10 1195
Fragments arranged by size:
1195 261 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 955 1500
Size: 955 545
BclI T'GATC_A
Cuts at: 0 1397 1500
Size: 1397 103
BglI GCCn_nnn'nGGC
Cuts at: 0 736 745 1500
Size: 736 9 755
Fragments arranged by size:
755 736 9
Bme1580I G_kGCm'C
Cuts at: 0 750 828 1313 1382 1478 1500
Size: 750 78 485 69 96 22
Fragments arranged by size:
750 485 96 78 69 22
BmgBI CAC'GTC
Cuts at: 0 1135 1473 1500
Size: 1135 338 27
Fragments arranged by size:
1135 338 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 1500
Size: 344 1156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 787 808 1500
Size: 382 405 21 692
Fragments arranged by size:
692 405 382 21
Bpu10I CC'TnA_GC
Cuts at: 0 1165 1432 1500
Size: 1165 267 68
Fragments arranged by size:
1165 267 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1500
Size: 883 617
BsaI GGTCTCn'nnnn_
Cuts at: 0 493 1172 1500
Size: 493 679 328
Fragments arranged by size:
679 493 328
BsaWI w'CCGG_w
Cuts at: 0 752 1500
Size: 752 748
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 1500
Size: 226 30 1244
Fragments arranged by size:
1244 226 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 481 805 823 1138 1141 1344 1500
Size: 7 474 324 18 315 3 203 156
Fragments arranged by size:
474 324 315 203 156 18 7 3
BseYI C'CCAG_C
Cuts at: 0 726 1500
Size: 726 774
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 711 766 1500
Size: 349 362 55 734
Fragments arranged by size:
734 362 349 55
BsiEI CG_ry'CG
Cuts at: 0 633 1500
Size: 633 867
BsiHKAI G_wGCw'C
Cuts at: 0 398 938 1382 1500
Size: 398 540 444 118
Fragments arranged by size:
540 444 398 118
Bsp1286I G_dGCh'C
Cuts at: 0 398 750 828 938 1171 1313 1382 1478
Size: 398 352 78 110 233 142 69 96
Cuts at: 1478 1500
Size: 22
Fragments arranged by size:
398 352 233 142 110 96 78 69
22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 785 1136 1427 1500
Size: 203 582 351 291 73
Fragments arranged by size:
582 351 291 203 73
BsrFI r'CCGG_y
Cuts at: 0 523 632 1500
Size: 523 109 868
Fragments arranged by size:
868 523 109
BssSI C'ACGA_G
Cuts at: 0 162 478 1500
Size: 162 316 1022
Fragments arranged by size:
1022 316 162
BstEII G'GTnAC_C
Cuts at: 0 1138 1500
Size: 1138 362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1104 1500
Size: 1104 396
BstYI r'GATC_y
Cuts at: 0 1415 1500
Size: 1415 85
BstZ17I GTA'TAC
Cuts at: 0 422 1500
Size: 422 1078
Bsu36I CC'TnA_GG
Cuts at: 0 1045 1500
Size: 1045 455
BtgI C'CryG_G
Cuts at: 0 654 927 1103 1500
Size: 654 273 176 397
Fragments arranged by size:
654 397 273 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 464 1500
Size: 464 1036
BtsI GCAGTG_nn'
Cuts at: 0 443 474 699 1317 1500
Size: 443 31 225 618 183
Fragments arranged by size:
618 443 225 183 31
DraI TTT'AAA
Cuts at: 0 1212 1500
Size: 1212 288
DraIII CAC_nnn'GTG
Cuts at: 0 1473 1500
Size: 1473 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 1477 1500
Size: 1477 23
EaeI y'GGCC_r
Cuts at: 0 29 737 1094 1500
Size: 29 708 357 406
Fragments arranged by size:
708 406 357 29
EarI CTCTTCn'nnn_
Cuts at: 0 386 895 1159 1500
Size: 386 509 264 341
Fragments arranged by size:
509 386 341 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1483 1500
Size: 1483 17
EcoICRI GAG'CTC
Cuts at: 0 396 936 1500
Size: 396 540 564
Fragments arranged by size:
564 540 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 787 808 929 1300 1500
Size: 382 405 21 121 371 200
Fragments arranged by size:
405 382 371 200 121 21
EcoO109I rG'GnC_Cy
Cuts at: 0 825 865 1500
Size: 825 40 635
Fragments arranged by size:
825 635 40
EcoRI G'AATT_C
Cuts at: 0 1120 1500
Size: 1120 380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1272 1500
Size: 1272 228
HaeII r_GCGC'y
Cuts at: 0 556 1500
Size: 556 944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 969 1001 1500
Size: 226 32 711 32 499
Fragments arranged by size:
711 499 226 32 32
HincII GTy'rAC
Cuts at: 0 1344 1500
Size: 1344 156
MscI TGG'CCA
Cuts at: 0 739 1096 1500
Size: 739 357 404
Fragments arranged by size:
739 404 357
MslI CAynn'nnrTG
Cuts at: 0 166 293 1015 1102 1500
Size: 166 127 722 87 398
Fragments arranged by size:
722 398 166 127 87
MspA1I CmG'CkG
Cuts at: 0 601 840 871 929 1057 1500
Size: 601 239 31 58 128 443
Fragments arranged by size:
601 443 239 128 58 31
NaeI GCC'GGC
Cuts at: 0 525 1500
Size: 525 975
NcoI C'CATG_G
Cuts at: 0 1103 1500
Size: 1103 397
NgoMIV G'CCGG_C
Cuts at: 0 523 1500
Size: 523 977
NspI r_CATG'y
Cuts at: 0 690 1296 1500
Size: 690 606 204
Fragments arranged by size:
690 606 204
PasI CC'CwG_GG
Cuts at: 0 1298 1500
Size: 1298 202
PciI A'CATG_T
Cuts at: 0 686 1500
Size: 686 814
PfoI T'CCnGG_A
Cuts at: 0 230 1500
Size: 230 1270
PpuMI rG'GwC_Cy
Cuts at: 0 865 1500
Size: 865 635
PspOMI G'GGCC_C
Cuts at: 0 746 824 1500
Size: 746 78 676
Fragments arranged by size:
746 676 78
PstI C_TGCA'G
Cuts at: 0 839 1500
Size: 839 661
PvuII CAG'CTG
Cuts at: 0 601 840 1057 1500
Size: 601 239 217 443
Fragments arranged by size:
601 443 239 217
SacI G_AGCT'C
Cuts at: 0 398 938 1500
Size: 398 540 562
Fragments arranged by size:
562 540 398
SacII CC_GC'GG
Cuts at: 0 930 1500
Size: 930 570
SanDI GG'GwC_CC
Cuts at: 0 865 1500
Size: 865 635
SapI GCTCTTCn'nnn_
Cuts at: 0 895 1500
Size: 895 605
SexAI A'CCwGG_T
Cuts at: 0 78 1500
Size: 78 1422
SfcI C'TryA_G
Cuts at: 0 455 504 835 1500
Size: 455 49 331 665
Fragments arranged by size:
665 455 331 49
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 745 1500
Size: 745 755
SmlI C'TyrA_G
Cuts at: 0 898 937 1500
Size: 898 39 563
Fragments arranged by size:
898 563 39
StyI C'CwwG_G
Cuts at: 0 128 1103 1500
Size: 128 975 397
Fragments arranged by size:
975 397 128
SwaI ATTT'AAAT
Cuts at: 0 1212 1500
Size: 1212 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 787 1438 1500
Size: 580 207 651 62
Fragments arranged by size:
651 580 207 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 673 705 1500
Size: 673 32 795
Fragments arranged by size:
795 673 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 1500
Size: 589 911
XbaI T'CTAG_A
Cuts at: 0 1002 1500
Size: 1002 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1500
Size: 306 1194
XhoI C'TCGA_G
Cuts at: 0 937 1500
Size: 937 563
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BbvCI BcgI BciVI BclI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BssSI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SapI SexAI SfcI SfiI SmlI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BfrBI BglII BlpI BmtI BplI BsaAI BsaBI
BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI ClaI CspCI EagI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SbfI ScaI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI TatI XmaI XmnI ZraI