Restriction map for
pFN21AE1943
(Linear) (Six-base) MAPSORT of: KIEE1943.seq Check: 6330 from: 1 to: 1494
KIEE1943.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AccI GT'mk_AC
Cuts at: 0 67 1494
Size: 67 1427
AflIII A'CryG_T
Cuts at: 0 140 1494
Size: 140 1354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 954 1494
Size: 954 540
AlwNI CAG_nnn'CTG
Cuts at: 0 1037 1494
Size: 1037 457
ApoI r'AATT_y
Cuts at: 0 892 1494
Size: 892 602
AseI AT'TA_AT
Cuts at: 0 463 1494
Size: 463 1031
BanI G'GyrC_C
Cuts at: 0 922 1494
Size: 922 572
BbsI GAAGACnn'nnnn_
Cuts at: 0 1317 1494
Size: 1317 177
BbvCI CC'TCA_GC
Cuts at: 0 917 1064 1494
Size: 917 147 430
Fragments arranged by size:
917 430 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1003 1037 1494
Size: 1003 34 457
Fragments arranged by size:
1003 457 34
BclI T'GATC_A
Cuts at: 0 372 1494
Size: 372 1122
BglII A'GATC_T
Cuts at: 0 1429 1494
Size: 1429 65
Bme1580I G_kGCm'C
Cuts at: 0 967 1494
Size: 967 527
BmgBI CAC'GTC
Cuts at: 0 141 1494
Size: 141 1353
Bpu10I CC'TnA_GC
Cuts at: 0 917 1064 1494
Size: 917 147 430
Fragments arranged by size:
917 430 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 1009 1494
Size: 1009 485
BseYI C'CCAG_C
Cuts at: 0 29 1494
Size: 29 1465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1424 1494
Size: 1424 70
BsiEI CG_ry'CG
Cuts at: 0 25 162 1494
Size: 25 137 1332
Fragments arranged by size:
1332 137 25
Bsp1286I G_dGCh'C
Cuts at: 0 967 1494
Size: 967 527
BspHI T'CATG_A
Cuts at: 0 986 1494
Size: 986 508
BsrBI CCG'CTC
Cuts at: 0 18 1494
Size: 18 1476
BsrDI GCAATG_nn'
Cuts at: 0 290 572 926 936 1019 1494
Size: 290 282 354 10 83 475
Fragments arranged by size:
475 354 290 282 83 10
BsrGI T'GTAC_A
Cuts at: 0 210 1494
Size: 210 1284
BstAPI GCAn_nnn'nTGC
Cuts at: 0 394 1494
Size: 394 1100
BstEII G'GTnAC_C
Cuts at: 0 715 1494
Size: 715 779
BstYI r'GATC_y
Cuts at: 0 89 1034 1429 1494
Size: 89 945 395 65
Fragments arranged by size:
945 395 89 65
BtsI GCAGTG_nn'
Cuts at: 0 1407 1455 1494
Size: 1407 48 39
Fragments arranged by size:
1407 48 39
DraI TTT'AAA
Cuts at: 0 647 1494
Size: 647 847
DraIII CAC_nnn'GTG
Cuts at: 0 1282 1494
Size: 1282 212
EcoO109I rG'GnC_Cy
Cuts at: 0 126 1494
Size: 126 1368
EcoRI G'AATT_C
Cuts at: 0 892 1494
Size: 892 602
EcoRV GAT'ATC
Cuts at: 0 903 1494
Size: 903 591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 967 1494
Size: 967 527
HaeII r_GCGC'y
Cuts at: 0 60 1494
Size: 60 1434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 1047 1079 1494
Size: 7 32 1008 32 415
Fragments arranged by size:
1008 415 32 32 7
HincII GTy'rAC
Cuts at: 0 68 1494
Size: 68 1426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 1494
Size: 387 1107
MslI CAynn'nnrTG
Cuts at: 0 334 1494
Size: 334 1160
PflMI CCAn_nnn'nTGG
Cuts at: 0 1025 1100 1494
Size: 1025 75 394
Fragments arranged by size:
1025 394 75
PpuMI rG'GwC_Cy
Cuts at: 0 126 1494
Size: 126 1368
PstI C_TGCA'G
Cuts at: 0 1042 1494
Size: 1042 452
PvuI CG_AT'CG
Cuts at: 0 162 1494
Size: 162 1332
SalI G'TCGA_C
Cuts at: 0 66 1494
Size: 66 1428
SanDI GG'GwC_CC
Cuts at: 0 126 1494
Size: 126 1368
SfcI C'TryA_G
Cuts at: 0 183 1038 1494
Size: 183 855 456
Fragments arranged by size:
855 456 183
SspI AAT'ATT
Cuts at: 0 461 751 1494
Size: 461 290 743
Fragments arranged by size:
743 461 290
StyI C'CwwG_G
Cuts at: 0 951 1494
Size: 951 543
SwaI ATTT'AAAT
Cuts at: 0 647 1494
Size: 647 847
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 680 1494
Size: 680 814
TatI w'GTAC_w
Cuts at: 0 210 933 1494
Size: 210 723 561
Fragments arranged by size:
723 561 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1434 1466 1494
Size: 1434 32 28
Fragments arranged by size:
1434 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1101 1494
Size: 1101 393
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApoI AseI BanI BbsI
BbvCI BcgI BclI BglII Bme1580I BmgBI Bpu10I BsaI
BseYI BsgI BsiEI Bsp1286I BspHI BsrBI BsrDI BsrGI
BstAPI BstEII BstYI BtsI DraI DraIII EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII MmeI MslI PflMI
PpuMI PstI PvuI SalI SanDI SfcI SspI StyI
SwaI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BciVI BfrBI BglI
BlpI BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrFI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI Tth111I XbaI XhoI XmaI XmnI ZraI