Restriction map for
pFN21AE1942
(Linear) (Six-base) MAPSORT of: KIEE1942.seq Check: 4722 from: 1 to: 1488
KIEE1942.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 696 745 1488
Size: 696 49 743
Fragments arranged by size:
743 696 49
AccI GT'mk_AC
Cuts at: 0 137 1488
Size: 137 1351
AflIII A'CryG_T
Cuts at: 0 488 594 917 1488
Size: 488 106 323 571
Fragments arranged by size:
571 488 323 106
AleI CACnn'nnGTG
Cuts at: 0 235 1488
Size: 235 1253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 58 1354 1488
Size: 58 1296 134
Fragments arranged by size:
1296 134 58
AlwNI CAG_nnn'CTG
Cuts at: 0 60 339 1276 1428 1488
Size: 60 279 937 152 60
Fragments arranged by size:
937 279 152 60 60
ApaI G_GGCC'C
Cuts at: 0 224 504 1128 1488
Size: 224 280 624 360
Fragments arranged by size:
624 360 280 224
ApoI r'AATT_y
Cuts at: 0 166 1393 1488
Size: 166 1227 95
Fragments arranged by size:
1227 166 95
AseI AT'TA_AT
Cuts at: 0 1175 1488
Size: 1175 313
AvaI C'yCGr_G
Cuts at: 0 233 503 1120 1488
Size: 233 270 617 368
Fragments arranged by size:
617 368 270 233
BamHI G'GATC_C
Cuts at: 0 844 1488
Size: 844 644
BanI G'GyrC_C
Cuts at: 0 349 1488
Size: 349 1139
BanII G_rGCy'C
Cuts at: 0 224 291 504 1128 1488
Size: 224 67 213 624 360
Fragments arranged by size:
624 360 224 213 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 822 1137 1370 1488
Size: 822 315 233 118
Fragments arranged by size:
822 315 233 118
BbvCI CC'TCA_GC
Cuts at: 0 1255 1488
Size: 1255 233
BciVI GTATCCnnnnn_n'
Cuts at: 0 340 900 1488
Size: 340 560 588
Fragments arranged by size:
588 560 340
BglI GCCn_nnn'nGGC
Cuts at: 0 752 1192 1488
Size: 752 440 296
Fragments arranged by size:
752 440 296
BglII A'GATC_T
Cuts at: 0 1349 1488
Size: 1349 139
Bme1580I G_kGCm'C
Cuts at: 0 224 504 1054 1128 1488
Size: 224 280 550 74 360
Fragments arranged by size:
550 360 280 224 74
BmgBI CAC'GTC
Cuts at: 0 216 489 597 918 1488
Size: 216 273 108 321 570
Fragments arranged by size:
570 321 273 216 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 1070 1488
Size: 78 992 418
Fragments arranged by size:
992 418 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 115 1488
Size: 115 1373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1488
Size: 37 1451
Bpu10I CC'TnA_GC
Cuts at: 0 1255 1488
Size: 1255 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 1057 1488
Size: 942 115 431
Fragments arranged by size:
942 431 115
BsaAI yAC'GTr
Cuts at: 0 606 1488
Size: 606 882
BsaHI Gr'CG_yC
Cuts at: 0 515 1488
Size: 515 973
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 502 970 1488
Size: 84 418 468 518
Fragments arranged by size:
518 468 418 84
BsiHKAI G_wGCw'C
Cuts at: 0 1289 1488
Size: 1289 199
Bsp1286I G_dGCh'C
Cuts at: 0 224 291 504 1054 1128 1289 1488
Size: 224 67 213 550 74 161 199
Fragments arranged by size:
550 224 213 199 161 74 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 696 745 790 1488
Size: 696 49 45 698
Fragments arranged by size:
698 696 49 45
BsrDI GCAATG_nn'
Cuts at: 0 792 1242 1488
Size: 792 450 246
Fragments arranged by size:
792 450 246
BsrFI r'CCGG_y
Cuts at: 0 358 1488
Size: 358 1130
BssSI C'ACGA_G
Cuts at: 0 748 1488
Size: 748 740
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 782 791 1488
Size: 321 461 9 697
Fragments arranged by size:
697 461 321 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 237 537 825 1440 1488
Size: 123 114 300 288 615 48
Fragments arranged by size:
615 300 288 123 114 48
BstYI r'GATC_y
Cuts at: 0 844 1349 1488
Size: 844 505 139
Fragments arranged by size:
844 505 139
BtgI C'CryG_G
Cuts at: 0 1241 1488
Size: 1241 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 413 1488
Size: 413 1075
BtsI GCAGTG_nn'
Cuts at: 0 307 801 1488
Size: 307 494 687
Fragments arranged by size:
687 494 307
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1488
Size: 595 893
EaeI y'GGCC_r
Cuts at: 0 365 815 1155 1463 1488
Size: 365 450 340 308 25
Fragments arranged by size:
450 365 340 308 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1488
Size: 37 1451
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 1488
Size: 193 1295
EcoO109I rG'GnC_Cy
Cuts at: 0 6 57 220 422 467 1124 1425 1488
Size: 6 51 163 202 45 657 301 63
Fragments arranged by size:
657 301 202 163 63 51 45 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 1488
Size: 619 32 837
Fragments arranged by size:
837 619 32
HincII GTy'rAC
Cuts at: 0 138 249 1488
Size: 138 111 1239
Fragments arranged by size:
1239 138 111
MscI TGG'CCA
Cuts at: 0 367 1465 1488
Size: 367 1098 23
Fragments arranged by size:
1098 367 23
MslI CAynn'nnrTG
Cuts at: 0 235 451 535 698 1488
Size: 235 216 84 163 790
Fragments arranged by size:
790 235 216 163 84
MspA1I CmG'CkG
Cuts at: 0 339 512 1488
Size: 339 173 976
Fragments arranged by size:
976 339 173
PasI CC'CwG_GG
Cuts at: 0 984 1488
Size: 984 504
PflMI CCAn_nnn'nTGG
Cuts at: 0 1134 1488
Size: 1134 354
PfoI T'CCnGG_A
Cuts at: 0 637 647 1469 1488
Size: 637 10 822 19
Fragments arranged by size:
822 637 19 10
PpuMI rG'GwC_Cy
Cuts at: 0 6 422 1488
Size: 6 416 1066
Fragments arranged by size:
1066 416 6
PspOMI G'GGCC_C
Cuts at: 0 220 500 1124 1488
Size: 220 280 624 364
Fragments arranged by size:
624 364 280 220
PstI C_TGCA'G
Cuts at: 0 37 317 796 1424 1488
Size: 37 280 479 628 64
Fragments arranged by size:
628 479 280 64 37
SalI G'TCGA_C
Cuts at: 0 136 1488
Size: 136 1352
ScaI AGT'ACT
Cuts at: 0 955 1488
Size: 955 533
SexAI A'CCwGG_T
Cuts at: 0 122 824 1488
Size: 122 702 664
Fragments arranged by size:
702 664 122
SfcI C'TryA_G
Cuts at: 0 33 313 615 792 1420 1488
Size: 33 280 302 177 628 68
Fragments arranged by size:
628 302 280 177 68 33
SmaI CCC'GGG
Cuts at: 0 235 505 1122 1488
Size: 235 270 617 366
Fragments arranged by size:
617 366 270 235
SmlI C'TyrA_G
Cuts at: 0 957 1072 1488
Size: 957 115 416
Fragments arranged by size:
957 416 115
SrfI GCCC'GGGC
Cuts at: 0 505 1488
Size: 505 983
StuI AGG'CCT
Cuts at: 0 655 1488
Size: 655 833
StyI C'CwwG_G
Cuts at: 0 299 1488
Size: 299 1189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1216 1488
Size: 1216 272
TatI w'GTAC_w
Cuts at: 0 953 1488
Size: 953 535
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1354 1386 1488
Size: 1354 32 102
Fragments arranged by size:
1354 102 32
Tth111I GACn'n_nGTC
Cuts at: 0 826 1488
Size: 826 662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 1488
Size: 360 1128
XmaI C'CCGG_G
Cuts at: 0 233 503 1120 1488
Size: 233 270 617 368
Fragments arranged by size:
617 368 270 233
XmnI GAAnn'nnTTC
Cuts at: 0 184 886 1488
Size: 184 702 602
Fragments arranged by size:
702 602 184
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaI ApoI
AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I HincII MscI
MslI MspA1I PasI PflMI PfoI PpuMI PspOMI PstI
SalI ScaI SexAI SfcI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI
BclI BfrBI BlpI BmtI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XhoI ZraI