(Linear) (Six-base) MAPSORT of: KIEE1941.seq  Check: 8163  from: 1  to: 1473



KIEE1941.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:51  ..

AccI GT'mk_AC

Cuts at:      0     257    1473 
   Size:        257    1216
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     687     719    1473 
   Size:        687      32     754

  Fragments arranged by size: 

                 754     687      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     817    1255    1473 
   Size:        817     438     218

  Fragments arranged by size: 

                 817     438     218
 
ApoI r'AATT_y

Cuts at:      0     489    1314    1473 
   Size:        489     825     159

  Fragments arranged by size: 

                 825     489     159
 
AseI AT'TA_AT

Cuts at:      0    1073    1473 
   Size:       1073     400
 
BanI G'GyrC_C

Cuts at:      0     915    1473 
   Size:        915     558
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     133    1230    1374    1473 
   Size:        133    1097     144      99

  Fragments arranged by size: 

                1097     144     133      99
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     479    1473 
   Size:        479     994
 
BglII A'GATC_T

Cuts at:      0     879    1176    1473 
   Size:        879     297     297

  Fragments arranged by size: 

                 879     297     297
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1243    1473 
   Size:       1243     230
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1348    1473 
   Size:       1348     125
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334    1369    1473 
   Size:        334    1035     104

  Fragments arranged by size: 

                1035     334     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1337    1473 
   Size:       1337     136
 
BsaBI GATnn'nnATC

Cuts at:      0     329    1473 
   Size:        329    1144
 
BsaHI Gr'CG_yC

Cuts at:      0    1303    1473 
   Size:       1303     170
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     602    1473 
   Size:        602     871
 
BsmI GAATG_Cn'

Cuts at:      0     118    1473 
   Size:        118    1355
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     670    1473 
   Size:        670     803
 
BstYI r'GATC_y

Cuts at:      0     411     702     879    1176    1365    1473 
   Size:        411     291     177     297     189     108

  Fragments arranged by size: 

                 411     297     291     189     177     108
 
Bsu36I CC'TnA_GG

Cuts at:      0     359    1473 
   Size:        359    1114
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     461     496    1096    1131    1473 
   Size:        461      35     600      35     342

  Fragments arranged by size: 

                 600     461     342      35      35
 
EaeI y'GGCC_r

Cuts at:      0      38     482     515    1473 
   Size:         38     444      33     958

  Fragments arranged by size: 

                 958     444      38      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     397     600    1473 
   Size:        397     203     873

  Fragments arranged by size: 

                 873     397     203
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1348    1473 
   Size:       1348     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     710    1473 
   Size:        710     763
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1245    1473 
   Size:       1245     228
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1363    1473 
   Size:       1363     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1161    1193    1473 
   Size:       1161      32     280

  Fragments arranged by size: 

                1161     280      32
 
HincII GTy'rAC

Cuts at:      0     639    1169    1473 
   Size:        639     530     304

  Fragments arranged by size: 

                 639     530     304
 
HpaI GTT'AAC

Cuts at:      0     639    1473 
   Size:        639     834
 
MscI TGG'CCA

Cuts at:      0      40     484     517    1473 
   Size:         40     444      33     956

  Fragments arranged by size: 

                 956     444      40      33
 
MspA1I CmG'CkG

Cuts at:      0    1443    1473 
   Size:       1443      30
 
PfoI T'CCnGG_A

Cuts at:      0     239    1473 
   Size:        239    1234
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     371     403    1473 
   Size:        371      32    1070

  Fragments arranged by size: 

                1070     371      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1245    1473 
   Size:       1245     228
 
PsiI TTA'TAA

Cuts at:      0     851    1473 
   Size:        851     622
 
PvuII CAG'CTG

Cuts at:      0    1443    1473 
   Size:       1443      30
 
ScaI AGT'ACT

Cuts at:      0     205    1473 
   Size:        205    1268
 
SfcI C'TryA_G

Cuts at:      0     582    1159    1473 
   Size:        582     577     314

  Fragments arranged by size: 

                 582     577     314
 
SmlI C'TyrA_G

Cuts at:      0     313    1348    1473 
   Size:        313    1035     125

  Fragments arranged by size: 

                1035     313     125
 
SspI AAT'ATT

Cuts at:      0     512     523     688    1131    1449    1473 
   Size:        512      11     165     443     318      24

  Fragments arranged by size: 

                 512     443     318     165      24      11
 
StyI C'CwwG_G

Cuts at:      0    1224    1473 
   Size:       1224     249
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      96    1473 
   Size:         96    1377
 
TatI w'GTAC_w

Cuts at:      0     188     203     907    1473 
   Size:        188      15     704     566

  Fragments arranged by size: 

                 704     566     188      15
 
XbaI T'CTAG_A

Cuts at:      0     493    1473 
   Size:        493     980
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     422     607    1473 
   Size:        422     185     866

  Fragments arranged by size: 

                 866     422     185
 

 Enzymes that do cut:

     AccI     AloI    AlwNI     ApoI     AseI     BanI     BbsI    BciVI
    BglII     BmrI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BseRI
     BsmI   BstAPI    BstYI   Bsu36I    CspCI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I   HincII     HpaI     MscI   MspA1I
     PfoI     PpiI    PpuMI     PsiI    PvuII     ScaI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI