Restriction map for
pFN21AE1940
(Linear) (Six-base) MAPSORT of: KIEE1940.seq Check: 7630 from: 1 to: 1458
KIEE1940.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 655 1458
Size: 655 803
AccI GT'mk_AC
Cuts at: 0 770 1458
Size: 770 688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1138 1173 1304 1458
Size: 928 210 35 131 154
Fragments arranged by size:
928 210 154 131 35
AflIII A'CryG_T
Cuts at: 0 1091 1458
Size: 1091 367
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1458
Size: 1231 32 195
Fragments arranged by size:
1231 195 32
AlwNI CAG_nnn'CTG
Cuts at: 0 213 793 906 1083 1274 1314 1458
Size: 213 580 113 177 191 40 144
Fragments arranged by size:
580 213 191 177 144 113 40
ApaI G_GGCC'C
Cuts at: 0 234 810 1458
Size: 234 576 648
Fragments arranged by size:
648 576 234
ApoI r'AATT_y
Cuts at: 0 1069 1458
Size: 1069 389
AvaI C'yCGr_G
Cuts at: 0 16 854 1458
Size: 16 838 604
Fragments arranged by size:
838 604 16
BamHI G'GATC_C
Cuts at: 0 1024 1458
Size: 1024 434
BanI G'GyrC_C
Cuts at: 0 139 166 382 825 1458
Size: 139 27 216 443 633
Fragments arranged by size:
633 443 216 139 27
BanII G_rGCy'C
Cuts at: 0 126 153 234 673 810 1458
Size: 126 27 81 439 137 648
Fragments arranged by size:
648 439 137 126 81 27
BbeI G_GCGC'C
Cuts at: 0 143 386 1458
Size: 143 243 1072
Fragments arranged by size:
1072 243 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 1094 1179 1458
Size: 1094 85 279
Fragments arranged by size:
1094 279 85
BglI GCCn_nnn'nGGC
Cuts at: 0 380 1458
Size: 380 1078
BlpI GC'TnA_GC
Cuts at: 0 1264 1458
Size: 1264 194
Bme1580I G_kGCm'C
Cuts at: 0 169 234 810 1458
Size: 169 65 576 648
Fragments arranged by size:
648 576 169 65
BmgBI CAC'GTC
Cuts at: 0 1092 1294 1458
Size: 1092 202 164
Fragments arranged by size:
1092 202 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1405 1432 1458
Size: 381 1024 27 26
Fragments arranged by size:
1024 381 27 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 391 1458
Size: 391 1067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 469 517 1158 1458
Size: 64 405 48 641 300
Fragments arranged by size:
641 405 300 64 48
Bpu10I CC'TnA_GC
Cuts at: 0 677 1458
Size: 677 781
BsaAI yAC'GTr
Cuts at: 0 601 1458
Size: 601 857
BsaHI Gr'CG_yC
Cuts at: 0 140 383 683 923 1458
Size: 140 243 300 240 535
Fragments arranged by size:
535 300 243 240 140
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 159 189 1231 1261 1458
Size: 159 30 1042 30 197
Fragments arranged by size:
1042 197 159 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1132 1458
Size: 1132 326
BseYI C'CCAG_C
Cuts at: 0 135 199 615 1458
Size: 135 64 416 843
Fragments arranged by size:
843 416 135 64
BsiHKAI G_wGCw'C
Cuts at: 0 673 876 1458
Size: 673 203 582
Fragments arranged by size:
673 582 203
Bsp1286I G_dGCh'C
Cuts at: 0 126 153 169 234 673 810 876 1458
Size: 126 27 16 65 439 137 66 582
Fragments arranged by size:
582 439 137 126 66 65 27 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 281 282 332 655 1184 1458
Size: 281 1 50 323 529 274
Fragments arranged by size:
529 323 281 274 50 1
BsrBI CCG'CTC
Cuts at: 0 252 1458
Size: 252 1206
BsrFI r'CCGG_y
Cuts at: 0 1063 1458
Size: 1063 395
BssSI C'ACGA_G
Cuts at: 0 253 376 1458
Size: 253 123 1082
Fragments arranged by size:
1082 253 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1274 1458
Size: 1274 184
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1376 1458
Size: 1376 82
BstYI r'GATC_y
Cuts at: 0 1024 1458
Size: 1024 434
Bsu36I CC'TnA_GG
Cuts at: 0 286 1076 1458
Size: 286 790 382
Fragments arranged by size:
790 382 286
BtgI C'CryG_G
Cuts at: 0 944 1458
Size: 944 514
EaeI y'GGCC_r
Cuts at: 0 261 342 567 1427 1458
Size: 261 81 225 860 31
Fragments arranged by size:
860 261 225 81 31
EarI CTCTTCn'nnn_
Cuts at: 0 1142 1458
Size: 1142 316
EcoICRI GAG'CTC
Cuts at: 0 671 1458
Size: 671 787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 469 517 928 1138 1158 1173 1304
Size: 64 405 48 411 210 20 15 131
Cuts at: 1304 1458
Size: 154
Fragments arranged by size:
411 405 210 154 131 64 48 20
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 1114 1458
Size: 289 825 344
Fragments arranged by size:
825 344 289
EcoO109I rG'GnC_Cy
Cuts at: 0 22 112 210 231 807 1458
Size: 22 90 98 21 576 651
Fragments arranged by size:
651 576 98 90 22 21
FspI TGC'GCA
Cuts at: 0 328 1458
Size: 328 1130
HaeII r_GCGC'y
Cuts at: 0 143 386 1458
Size: 143 243 1072
Fragments arranged by size:
1072 243 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1296 1328 1458
Size: 1296 32 130
Fragments arranged by size:
1296 130 32
HincII GTy'rAC
Cuts at: 0 321 1458
Size: 321 1137
HpaI GTT'AAC
Cuts at: 0 321 1458
Size: 321 1137
KasI G'GCGC_C
Cuts at: 0 139 382 1458
Size: 139 243 1076
Fragments arranged by size:
1076 243 139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1307 1458
Size: 1307 151
MscI TGG'CCA
Cuts at: 0 569 1429 1458
Size: 569 860 29
Fragments arranged by size:
860 569 29
MspA1I CmG'CkG
Cuts at: 0 710 1263 1458
Size: 710 553 195
Fragments arranged by size:
710 553 195
NaeI GCC'GGC
Cuts at: 0 1065 1458
Size: 1065 393
NarI GG'CG_CC
Cuts at: 0 140 383 1458
Size: 140 243 1075
Fragments arranged by size:
1075 243 140
NcoI C'CATG_G
Cuts at: 0 944 1458
Size: 944 514
NgoMIV G'CCGG_C
Cuts at: 0 1063 1458
Size: 1063 395
NspI r_CATG'y
Cuts at: 0 477 615 1458
Size: 477 138 843
Fragments arranged by size:
843 477 138
PasI CC'CwG_GG
Cuts at: 0 207 659 1458
Size: 207 452 799
Fragments arranged by size:
799 452 207
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 1352 1458
Size: 576 776 106
Fragments arranged by size:
776 576 106
PfoI T'CCnGG_A
Cuts at: 0 1250 1458
Size: 1250 208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1458
Size: 1231 32 195
Fragments arranged by size:
1231 195 32
PpuMI rG'GwC_Cy
Cuts at: 0 22 1458
Size: 22 1436
PspOMI G'GGCC_C
Cuts at: 0 230 806 1458
Size: 230 576 652
Fragments arranged by size:
652 576 230
PspXI vC'TCGA_Gb
Cuts at: 0 16 1458
Size: 16 1442
PstI C_TGCA'G
Cuts at: 0 902 1458
Size: 902 556
PvuII CAG'CTG
Cuts at: 0 1263 1458
Size: 1263 195
SacI G_AGCT'C
Cuts at: 0 673 1458
Size: 673 785
SanDI GG'GwC_CC
Cuts at: 0 22 1458
Size: 22 1436
SapI GCTCTTCn'nnn_
Cuts at: 0 1142 1458
Size: 1142 316
SfcI C'TryA_G
Cuts at: 0 310 898 1458
Size: 310 588 560
Fragments arranged by size:
588 560 310
SfoI GGC'GCC
Cuts at: 0 141 384 1458
Size: 141 243 1074
Fragments arranged by size:
1074 243 141
SmaI CCC'GGG
Cuts at: 0 856 1458
Size: 856 602
SmlI C'TyrA_G
Cuts at: 0 16 1458
Size: 16 1442
SnaBI TAC'GTA
Cuts at: 0 601 1458
Size: 601 857
StyI C'CwwG_G
Cuts at: 0 944 1235 1458
Size: 944 291 223
Fragments arranged by size:
944 291 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 220 655 1458
Size: 220 435 803
Fragments arranged by size:
803 435 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 159 191 1326 1358 1458
Size: 159 32 1135 32 100
Fragments arranged by size:
1135 159 100 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1310 1458
Size: 1310 148
XhoI C'TCGA_G
Cuts at: 0 16 1458
Size: 16 1442
XmaI C'CCGG_G
Cuts at: 0 854 1458
Size: 854 604
XmnI GAAnn'nnTTC
Cuts at: 0 1151 1458
Size: 1151 307
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
HpaI KasI MmeI MscI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SanDI SapI SfcI SfoI
SmaI SmlI SnaBI StyI TaqII TstI Tth111I XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BfrBI BglII BmtI BpuEI BsaI
BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspAI HindIII KpnI MfeI
MluI MslI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XcmI ZraI