Restriction map for
pFN21AE1938
(Linear) (Six-base) MAPSORT of: KIEE1938.seq Check: 433 from: 1 to: 1440
KIEE1938.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1014 1440
Size: 971 43 426
Fragments arranged by size:
971 426 43
AleI CACnn'nnGTG
Cuts at: 0 749 1440
Size: 749 691
AlwNI CAG_nnn'CTG
Cuts at: 0 700 723 994 1037 1369 1440
Size: 700 23 271 43 332 71
Fragments arranged by size:
700 332 271 71 43 23
ApaI G_GGCC'C
Cuts at: 0 354 611 1440
Size: 354 257 829
Fragments arranged by size:
829 354 257
ApaLI G'TGCA_C
Cuts at: 0 964 1064 1440
Size: 964 100 376
Fragments arranged by size:
964 376 100
AvaI C'yCGr_G
Cuts at: 0 374 1166 1440
Size: 374 792 274
Fragments arranged by size:
792 374 274
BamHI G'GATC_C
Cuts at: 0 1409 1440
Size: 1409 31
BanII G_rGCy'C
Cuts at: 0 354 437 611 1440
Size: 354 83 174 829
Fragments arranged by size:
829 354 174 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 1440
Size: 476 964
BbvCI CC'TCA_GC
Cuts at: 0 658 1440
Size: 658 782
BfrBI ATG'CAT
Cuts at: 0 529 1440
Size: 529 911
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1440
Size: 859 581
BglII A'GATC_T
Cuts at: 0 1328 1440
Size: 1328 112
Bme1580I G_kGCm'C
Cuts at: 0 42 324 354 583 611 788 968 1068
Size: 42 282 30 229 28 177 180 100
Cuts at: 1068 1360 1440
Size: 292 80
Fragments arranged by size:
292 282 229 180 177 100 80 42
30 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 605 1440
Size: 605 835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 1350 1440
Size: 207 1143 90
Fragments arranged by size:
1143 207 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 346 722 1440
Size: 109 237 376 718
Fragments arranged by size:
718 376 237 109
Bpu10I CC'TnA_GC
Cuts at: 0 116 658 1440
Size: 116 542 782
Fragments arranged by size:
782 542 116
BsaAI yAC'GTr
Cuts at: 0 858 1324 1440
Size: 858 466 116
Fragments arranged by size:
858 466 116
BsaHI Gr'CG_yC
Cuts at: 0 7 85 1440
Size: 7 78 1355
Fragments arranged by size:
1355 78 7
BsaWI w'CCGG_w
Cuts at: 0 1406 1440
Size: 1406 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 780 810 1232 1262 1440
Size: 780 30 422 30 178
Fragments arranged by size:
780 422 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 719 1440
Size: 719 721
BseYI C'CCAG_C
Cuts at: 0 884 1059 1359 1440
Size: 884 175 300 81
Fragments arranged by size:
884 300 175 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 659 930 1440
Size: 472 187 271 510
Fragments arranged by size:
510 472 271 187
BsiHKAI G_wGCw'C
Cuts at: 0 383 437 716 968 1068 1440
Size: 383 54 279 252 100 372
Fragments arranged by size:
383 372 279 252 100 54
BsmI GAATG_Cn'
Cuts at: 0 571 1440
Size: 571 869
BsmBI CGTCTCn'nnnn_
Cuts at: 0 133 1042 1440
Size: 133 909 398
Fragments arranged by size:
909 398 133
Bsp1286I G_dGCh'C
Cuts at: 0 42 324 354 383 437 583 611 716
Size: 42 282 30 29 54 146 28 105
Cuts at: 716 788 968 1068 1360 1440
Size: 72 180 100 292 80
Fragments arranged by size:
292 282 180 146 105 100 80 72
54 42 30 29 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 566 1440
Size: 135 431 874
Fragments arranged by size:
874 431 135
BsrBI CCG'CTC
Cuts at: 0 65 1440
Size: 65 1375
BsrDI GCAATG_nn'
Cuts at: 0 863 1440
Size: 863 577
BsrFI r'CCGG_y
Cuts at: 0 69 1440
Size: 69 1371
BssHII G'CGCG_C
Cuts at: 0 54 1440
Size: 54 1386
BssSI C'ACGA_G
Cuts at: 0 635 1440
Size: 635 805
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1128 1440
Size: 1128 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1440
Size: 981 459
BstYI r'GATC_y
Cuts at: 0 414 821 1328 1409 1440
Size: 414 407 507 81 31
Fragments arranged by size:
507 414 407 81 31
BtgI C'CryG_G
Cuts at: 0 11 760 1148 1440
Size: 11 749 388 292
Fragments arranged by size:
749 388 292 11
BtsI GCAGTG_nn'
Cuts at: 0 324 822 1129 1370 1440
Size: 324 498 307 241 70
Fragments arranged by size:
498 324 307 241 70
ClaI AT'CG_AT
Cuts at: 0 1094 1440
Size: 1094 346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1105 1140 1440
Size: 1105 35 300
Fragments arranged by size:
1105 300 35
EaeI y'GGCC_r
Cuts at: 0 860 917 933 998 1260 1440
Size: 860 57 16 65 262 180
Fragments arranged by size:
860 262 180 65 57 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 814 1440
Size: 814 626
EcoICRI GAG'CTC
Cuts at: 0 435 1440
Size: 435 1005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 346 722 971 1014 1440
Size: 109 237 376 249 43 426
Fragments arranged by size:
426 376 249 237 109 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 1440
Size: 328 1112
EcoO109I rG'GnC_Cy
Cuts at: 0 350 607 675 877 1440
Size: 350 257 68 202 563
Fragments arranged by size:
563 350 257 202 68
HaeII r_GCGC'y
Cuts at: 0 124 406 1440
Size: 124 282 1034
Fragments arranged by size:
1034 282 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 530 562 1232 1264 1440
Size: 530 32 670 32 176
Fragments arranged by size:
670 530 176 32 32
MscI TGG'CCA
Cuts at: 0 862 919 1000 1440
Size: 862 57 81 440
Fragments arranged by size:
862 440 81 57
MslI CAynn'nnrTG
Cuts at: 0 359 749 833 1208 1440
Size: 359 390 84 375 232
Fragments arranged by size:
390 375 359 232 84
MspA1I CmG'CkG
Cuts at: 0 700 1440
Size: 700 740
NaeI GCC'GGC
Cuts at: 0 71 1440
Size: 71 1369
NcoI C'CATG_G
Cuts at: 0 760 1440
Size: 760 680
NdeI CA'TA_TG
Cuts at: 0 768 1440
Size: 768 672
NgoMIV G'CCGG_C
Cuts at: 0 69 1440
Size: 69 1371
NsiI A_TGCA'T
Cuts at: 0 531 1440
Size: 531 909
NspI r_CATG'y
Cuts at: 0 565 1425 1440
Size: 565 860 15
Fragments arranged by size:
860 565 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 1440
Size: 600 840
PmlI CAC'GTG
Cuts at: 0 858 1440
Size: 858 582
PpuMI rG'GwC_Cy
Cuts at: 0 877 1440
Size: 877 563
PspOMI G'GGCC_C
Cuts at: 0 350 607 1440
Size: 350 257 833
Fragments arranged by size:
833 350 257
PvuII CAG'CTG
Cuts at: 0 700 1440
Size: 700 740
SacI G_AGCT'C
Cuts at: 0 437 1440
Size: 437 1003
SfcI C'TryA_G
Cuts at: 0 366 459 1440
Size: 366 93 981
Fragments arranged by size:
981 366 93
SmaI CCC'GGG
Cuts at: 0 376 1168 1440
Size: 376 792 272
Fragments arranged by size:
792 376 272
SphI G_CATG'C
Cuts at: 0 565 1440
Size: 565 875
StyI C'CwwG_G
Cuts at: 0 410 760 1440
Size: 410 350 680
Fragments arranged by size:
680 410 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 664 1051 1097 1440
Size: 430 234 387 46 343
Fragments arranged by size:
430 387 343 234 46
TatI w'GTAC_w
Cuts at: 0 1340 1440
Size: 1340 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 780 812 1440
Size: 780 32 628
Fragments arranged by size:
780 628 32
XbaI T'CTAG_A
Cuts at: 0 1206 1440
Size: 1206 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1440
Size: 207 1233
XmaI C'CCGG_G
Cuts at: 0 374 1166 1440
Size: 374 792 274
Fragments arranged by size:
792 374 274
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApaLI AvaI BamHI BanII
BbsI BbvCI BfrBI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
ClaI CspCI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI NspI PflMI PmlI PpuMI PspOMI PvuII
SacI SfcI SmaI SphI StyI TaqII TatI TstI
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BlpI
BmgBI BmtI BpuEI BsaI BsaBI BsiEI BsiWI BspEI
BspHI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI DraI
DraIII DrdI EagI EarI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NheI NotI NruI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XhoI XmnI
ZraI