Restriction map for
pFN21AE1931
(Linear) (Six-base) MAPSORT of: KIEE1931.seq Check: 4962 from: 1 to: 1335
KIEE1931.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AatII G_ACGT'C
Cuts at: 0 248 1335
Size: 248 1087
AccI GT'mk_AC
Cuts at: 0 316 352 1001 1335
Size: 316 36 649 334
Fragments arranged by size:
649 334 316 36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 892 1335
Size: 41 851 443
Fragments arranged by size:
851 443 41
AflIII A'CryG_T
Cuts at: 0 374 428 1335
Size: 374 54 907
Fragments arranged by size:
907 374 54
AleI CACnn'nnGTG
Cuts at: 0 831 1335
Size: 831 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 1335
Size: 128 32 1175
Fragments arranged by size:
1175 128 32
ApaI G_GGCC'C
Cuts at: 0 460 1063 1221 1327 1335
Size: 460 603 158 106 8
Fragments arranged by size:
603 460 158 106 8
AvaI C'yCGr_G
Cuts at: 0 684 1335
Size: 684 651
BanI G'GyrC_C
Cuts at: 0 1240 1335
Size: 1240 95
BanII G_rGCy'C
Cuts at: 0 73 122 460 864 1063 1221 1327 1335
Size: 73 49 338 404 199 158 106 8
Fragments arranged by size:
404 338 199 158 106 73 49 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1207 1335
Size: 1207 128
BbvCI CC'TCA_GC
Cuts at: 0 934 1335
Size: 934 401
BglI GCCn_nnn'nGGC
Cuts at: 0 929 1116 1335
Size: 929 187 219
Fragments arranged by size:
929 219 187
BglII A'GATC_T
Cuts at: 0 701 1335
Size: 701 634
BlpI GC'TnA_GC
Cuts at: 0 744 1335
Size: 744 591
Bme1580I G_kGCm'C
Cuts at: 0 460 925 1019 1063 1221 1327 1335
Size: 460 465 94 44 158 106 8
Fragments arranged by size:
465 460 158 106 94 44 8
BmgBI CAC'GTC
Cuts at: 0 431 1020 1188 1335
Size: 431 589 168 147
Fragments arranged by size:
589 431 168 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 912 1224 1335
Size: 912 312 111
Fragments arranged by size:
912 312 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 458 901 1335
Size: 34 424 443 434
Fragments arranged by size:
443 434 424 34
Bpu10I CC'TnA_GC
Cuts at: 0 934 1335
Size: 934 401
BsaHI Gr'CG_yC
Cuts at: 0 245 1335
Size: 245 1090
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 629 659 1335
Size: 244 30 355 30 676
Fragments arranged by size:
676 355 244 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 130 455 1335
Size: 75 55 325 880
Fragments arranged by size:
880 325 75 55
BseYI C'CCAG_C
Cuts at: 0 749 887 952 957 1037 1335
Size: 749 138 65 5 80 298
Fragments arranged by size:
749 298 138 80 65 5
BsiEI CG_ry'CG
Cuts at: 0 220 1335
Size: 220 1115
BsiHKAI G_wGCw'C
Cuts at: 0 475 1335
Size: 475 860
BsmBI CGTCTCn'nnnn_
Cuts at: 0 279 1335
Size: 279 1056
Bsp1286I G_dGCh'C
Cuts at: 0 73 122 460 475 864 925 1019 1063
Size: 73 49 338 15 389 61 94 44
Cuts at: 1063 1221 1327 1335
Size: 158 106 8
Fragments arranged by size:
389 338 158 106 94 73 61 49
44 15 8
BsrBI CCG'CTC
Cuts at: 0 270 392 586 743 1056 1335
Size: 270 122 194 157 313 279
Fragments arranged by size:
313 279 270 194 157 122
BsrDI GCAATG_nn'
Cuts at: 0 727 1335
Size: 727 608
BsrFI r'CCGG_y
Cuts at: 0 188 215 512 843 1046 1132 1288 1335
Size: 188 27 297 331 203 86 156 47
Fragments arranged by size:
331 297 203 188 156 86 47 27
BsrGI T'GTAC_A
Cuts at: 0 722 1335
Size: 722 613
BstYI r'GATC_y
Cuts at: 0 701 1335
Size: 701 634
BstZ17I GTA'TAC
Cuts at: 0 317 353 1335
Size: 317 36 982
Fragments arranged by size:
982 317 36
BtgI C'CryG_G
Cuts at: 0 294 926 963 1023 1032 1236 1335
Size: 294 632 37 60 9 204 99
Fragments arranged by size:
632 294 204 99 60 37 9
BtsI GCAGTG_nn'
Cuts at: 0 826 1228 1335
Size: 826 402 107
Fragments arranged by size:
826 402 107
EaeI y'GGCC_r
Cuts at: 0 387 514 587 966 1122 1182 1335
Size: 387 127 73 379 156 60 153
Fragments arranged by size:
387 379 156 153 127 73 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 612 1297 1335
Size: 612 685 38
Fragments arranged by size:
685 612 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 41 458 892 901 1335
Size: 34 7 417 434 9 434
Fragments arranged by size:
434 434 417 34 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 440 1335
Size: 440 895
EcoO109I rG'GnC_Cy
Cuts at: 0 29 456 457 1059 1060 1099 1277 1323
Size: 29 427 1 602 1 39 178 46
Cuts at: 1323 1335
Size: 12
Fragments arranged by size:
602 427 178 46 39 29 12 1
1
FseI GG_CCGG'CC
Cuts at: 0 1136 1335
Size: 1136 199
FspI TGC'GCA
Cuts at: 0 104 1335
Size: 104 1231
HaeII r_GCGC'y
Cuts at: 0 979 1335
Size: 979 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 1335
Size: 727 32 576
Fragments arranged by size:
727 576 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1335
Size: 806 529
MscI TGG'CCA
Cuts at: 0 1124 1335
Size: 1124 211
MslI CAynn'nnrTG
Cuts at: 0 373 831 1022 1335
Size: 373 458 191 313
Fragments arranged by size:
458 373 313 191
MspA1I CmG'CkG
Cuts at: 0 544 756 891 1010 1329 1335
Size: 544 212 135 119 319 6
Fragments arranged by size:
544 319 212 135 119 6
NaeI GCC'GGC
Cuts at: 0 190 514 1048 1134 1335
Size: 190 324 534 86 201
Fragments arranged by size:
534 324 201 190 86
NcoI C'CATG_G
Cuts at: 0 1023 1335
Size: 1023 312
NgoMIV G'CCGG_C
Cuts at: 0 188 512 1046 1132 1335
Size: 188 324 534 86 203
Fragments arranged by size:
534 324 203 188 86
NspI r_CATG'y
Cuts at: 0 378 1335
Size: 378 957
PasI CC'CwG_GG
Cuts at: 0 995 1150 1335
Size: 995 155 185
Fragments arranged by size:
995 185 155
PciI A'CATG_T
Cuts at: 0 374 1335
Size: 374 961
PflMI CCAn_nnn'nTGG
Cuts at: 0 964 1335
Size: 964 371
PfoI T'CCnGG_A
Cuts at: 0 325 1335
Size: 325 1010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1335
Size: 55 32 1248
Fragments arranged by size:
1248 55 32
PpuMI rG'GwC_Cy
Cuts at: 0 29 1335
Size: 29 1306
PspOMI G'GGCC_C
Cuts at: 0 456 1059 1217 1323 1335
Size: 456 603 158 106 12
Fragments arranged by size:
603 456 158 106 12
PstI C_TGCA'G
Cuts at: 0 794 1335
Size: 794 541
SanDI GG'GwC_CC
Cuts at: 0 29 1335
Size: 29 1306
SfcI C'TryA_G
Cuts at: 0 790 1335
Size: 790 545
SgrAI Cr'CCGG_yG
Cuts at: 0 188 843 1335
Size: 188 655 492
Fragments arranged by size:
655 492 188
SmaI CCC'GGG
Cuts at: 0 686 1335
Size: 686 649
StyI C'CwwG_G
Cuts at: 0 278 573 1023 1335
Size: 278 295 450 312
Fragments arranged by size:
450 312 295 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 327 696 1258 1335
Size: 327 369 562 77
Fragments arranged by size:
562 369 327 77
TatI w'GTAC_w
Cuts at: 0 722 1335
Size: 722 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 629 661 1335
Size: 244 32 353 32 674
Fragments arranged by size:
674 353 244 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 639 1335
Size: 639 696
XmaI C'CCGG_G
Cuts at: 0 684 1335
Size: 684 651
ZraI GAC'GTC
Cuts at: 0 246 1335
Size: 246 1089
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AloI ApaI AvaI
BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI
BstYI BstZ17I BtgI BtsI EaeI EciI Eco57MI EcoNI
EcoO109I FseI FspI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PstI SanDI SfcI SgrAI
SmaI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmI
BspEI BspHI BspMI BssHII BssSI BstAPI BstBI BstEII
BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmnI