Restriction map for
pFN21AE1929
(Linear) (Six-base) MAPSORT of: KIEE1929.seq Check: 2900 from: 1 to: 1401
KIEE1929.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
Acc65I G'GTAC_C
Cuts at: 0 176 1401
Size: 176 1225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 939 1401
Size: 456 483 462
Fragments arranged by size:
483 462 456
AfeI AGC'GCT
Cuts at: 0 1167 1401
Size: 1167 234
AleI CACnn'nnGTG
Cuts at: 0 1366 1401
Size: 1366 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 661 1401
Size: 661 740
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1401
Size: 1108 32 261
Fragments arranged by size:
1108 261 32
AvaI C'yCGr_G
Cuts at: 0 12 310 876 1058 1401
Size: 12 298 566 182 343
Fragments arranged by size:
566 343 298 182 12
BanI G'GyrC_C
Cuts at: 0 176 196 1351 1401
Size: 176 20 1155 50
Fragments arranged by size:
1155 176 50 20
BanII G_rGCy'C
Cuts at: 0 607 848 1059 1121 1217 1261 1401
Size: 607 241 211 62 96 44 140
Fragments arranged by size:
607 241 211 140 96 62 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1101 1401
Size: 1101 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 975 1009 1401
Size: 975 34 392
Fragments arranged by size:
975 392 34
BglI GCCn_nnn'nGGC
Cuts at: 0 353 1401
Size: 353 1048
BglII A'GATC_T
Cuts at: 0 212 1401
Size: 212 1189
Bme1580I G_kGCm'C
Cuts at: 0 581 909 1356 1401
Size: 581 328 447 45
Fragments arranged by size:
581 447 328 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 1264 1401
Size: 1264 137
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 753 1401
Size: 753 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 539 757 1216 1401
Size: 134 405 218 459 185
Fragments arranged by size:
459 405 218 185 134
BsaAI yAC'GTr
Cuts at: 0 904 1130 1401
Size: 904 226 271
Fragments arranged by size:
904 271 226
BsaHI Gr'CG_yC
Cuts at: 0 119 227 698 1045 1401
Size: 119 108 471 347 356
Fragments arranged by size:
471 356 347 119 108
BsaWI w'CCGG_w
Cuts at: 0 317 1401
Size: 317 1084
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 157 187 674 704 1108 1138
Size: 107 30 20 30 487 30 404 30
Cuts at: 1138 1401
Size: 263
Fragments arranged by size:
487 404 263 107 30 30 30 30
20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 760 1401
Size: 416 344 641
Fragments arranged by size:
641 416 344
BseYI C'CCAG_C
Cuts at: 0 827 975 1048 1401
Size: 827 148 73 353
Fragments arranged by size:
827 353 148 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1221 1401
Size: 631 590 180
Fragments arranged by size:
631 590 180
BsiEI CG_ry'CG
Cuts at: 0 366 806 1401
Size: 366 440 595
Fragments arranged by size:
595 440 366
BsiHKAI G_wGCw'C
Cuts at: 0 607 1217 1373 1401
Size: 607 610 156 28
Fragments arranged by size:
610 607 156 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 110 793 1401
Size: 110 683 608
Fragments arranged by size:
683 608 110
Bsp1286I G_dGCh'C
Cuts at: 0 581 607 848 909 1059 1121 1217 1261
Size: 581 26 241 61 150 62 96 44
Cuts at: 1261 1356 1373 1401
Size: 95 17 28
Fragments arranged by size:
581 241 150 96 95 62 61 44
28 26 17
BspEI T'CCGG_A
Cuts at: 0 317 1401
Size: 317 1084
BsrBI CCG'CTC
Cuts at: 0 1200 1401
Size: 1200 201
BssHII G'CGCG_C
Cuts at: 0 64 1401
Size: 64 1337
BstXI CCAn_nnnn'nTGG
Cuts at: 0 444 1401
Size: 444 957
BstYI r'GATC_y
Cuts at: 0 212 320 756 1401
Size: 212 108 436 645
Fragments arranged by size:
645 436 212 108
BtgI C'CryG_G
Cuts at: 0 536 741 1089 1401
Size: 536 205 348 312
Fragments arranged by size:
536 348 312 205
BtsI GCAGTG_nn'
Cuts at: 0 581 888 1401
Size: 581 307 513
Fragments arranged by size:
581 513 307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 420 455 1401
Size: 420 35 946
Fragments arranged by size:
946 420 35
EarI CTCTTCn'nnn_
Cuts at: 0 958 1401
Size: 958 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 10 1401
Size: 10 1391
EcoICRI GAG'CTC
Cuts at: 0 605 1215 1401
Size: 605 610 186
Fragments arranged by size:
610 605 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 456 539 757 939 1216 1401
Size: 134 322 83 218 182 277 185
Fragments arranged by size:
322 277 218 185 182 134 83
EcoO109I rG'GnC_Cy
Cuts at: 0 279 629 948 1027 1401
Size: 279 350 319 79 374
Fragments arranged by size:
374 350 319 279 79
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 1401
Size: 156 1245
HaeII r_GCGC'y
Cuts at: 0 735 842 1169 1401
Size: 735 107 327 232
Fragments arranged by size:
735 327 232 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 741 773 1401
Size: 741 32 628
Fragments arranged by size:
741 628 32
HincII GTy'rAC
Cuts at: 0 84 1401
Size: 84 1317
KpnI G_GTAC'C
Cuts at: 0 180 1401
Size: 180 1221
MslI CAynn'nnrTG
Cuts at: 0 442 670 1366 1401
Size: 442 228 696 35
Fragments arranged by size:
696 442 228 35
MspA1I CmG'CkG
Cuts at: 0 26 598 827 959 1401
Size: 26 572 229 132 442
Fragments arranged by size:
572 442 229 132 26
NcoI C'CATG_G
Cuts at: 0 536 1089 1401
Size: 536 553 312
Fragments arranged by size:
553 536 312
NspI r_CATG'y
Cuts at: 0 162 1401
Size: 162 1239
PfoI T'CCnGG_A
Cuts at: 0 53 692 1112 1154 1401
Size: 53 639 420 42 247
Fragments arranged by size:
639 420 247 53 42
PmlI CAC'GTG
Cuts at: 0 1130 1401
Size: 1130 271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1401
Size: 1108 32 261
Fragments arranged by size:
1108 261 32
PpuMI rG'GwC_Cy
Cuts at: 0 279 1027 1401
Size: 279 748 374
Fragments arranged by size:
748 374 279
PshAI GACnn'nnGTC
Cuts at: 0 332 1401
Size: 332 1069
PvuII CAG'CTG
Cuts at: 0 598 959 1401
Size: 598 361 442
Fragments arranged by size:
598 442 361
SacI G_AGCT'C
Cuts at: 0 607 1217 1401
Size: 607 610 184
Fragments arranged by size:
610 607 184
SexAI A'CCwGG_T
Cuts at: 0 281 1401
Size: 281 1120
SfcI C'TryA_G
Cuts at: 0 616 1401
Size: 616 785
SmaI CCC'GGG
Cuts at: 0 1060 1401
Size: 1060 341
SphI G_CATG'C
Cuts at: 0 162 1401
Size: 162 1239
StyI C'CwwG_G
Cuts at: 0 536 1089 1364 1401
Size: 536 553 275 37
Fragments arranged by size:
553 536 275 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 989 1401
Size: 549 440 412
Fragments arranged by size:
549 440 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 157 189 674 706 1401
Size: 107 32 18 32 485 32 695
Fragments arranged by size:
695 485 107 32 32 32 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 444 1401
Size: 381 63 957
Fragments arranged by size:
957 381 63
XmaI C'CCGG_G
Cuts at: 0 1058 1401
Size: 1058 343
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlfI AloI AvaI BanI
BanII BbsI BcgI BglI BglII Bme1580I BmrI BplI
BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BssHII BstXI
BstYI BtgI BtsI CspCI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HincII KpnI MslI MspA1I
NcoI NspI PfoI PmlI PpiI PpuMI PshAI PvuII
SacI SexAI SfcI SmaI SphI StyI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BlpI
BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsiWI BsmI
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EaeI EagI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI