Restriction map for
pFN21AE1928
(Linear) (Six-base) MAPSORT of: KIEE1928.seq Check: 9997 from: 1 to: 1398
KIEE1928.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 468 1398
Size: 468 930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1398
Size: 565 833
AhdI GACnn_n'nnGTC
Cuts at: 0 636 1398
Size: 636 762
AleI CACnn'nnGTG
Cuts at: 0 1139 1398
Size: 1139 259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 1398
Size: 145 1253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 743 775 1398
Size: 743 32 623
Fragments arranged by size:
743 623 32
AlwNI CAG_nnn'CTG
Cuts at: 0 327 732 1398
Size: 327 405 666
Fragments arranged by size:
666 405 327
AvaI C'yCGr_G
Cuts at: 0 51 168 223 403 1398
Size: 51 117 55 180 995
Fragments arranged by size:
995 180 117 55 51
BanI G'GyrC_C
Cuts at: 0 55 1398
Size: 55 1343
BanII G_rGCy'C
Cuts at: 0 76 404 1398
Size: 76 328 994
Fragments arranged by size:
994 328 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 24 58 1398
Size: 24 34 1340
Fragments arranged by size:
1340 34 24
Bme1580I G_kGCm'C
Cuts at: 0 58 224 1398
Size: 58 166 1174
Fragments arranged by size:
1174 166 58
BmgBI CAC'GTC
Cuts at: 0 649 1398
Size: 649 749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 391 1398
Size: 60 331 1007
Fragments arranged by size:
1007 331 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1398
Size: 397 1001
BsaAI yAC'GTr
Cuts at: 0 90 1398
Size: 90 1308
BsaHI Gr'CG_yC
Cuts at: 0 269 920 1398
Size: 269 651 478
Fragments arranged by size:
651 478 269
BsaWI w'CCGG_w
Cuts at: 0 908 956 1398
Size: 908 48 442
Fragments arranged by size:
908 442 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1240 1270 1398
Size: 1240 30 128
Fragments arranged by size:
1240 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 183 466 469 586 1234 1398
Size: 183 283 3 117 648 164
Fragments arranged by size:
648 283 183 164 117 3
BseYI C'CCAG_C
Cuts at: 0 136 1398
Size: 136 1262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1398
Size: 988 410
BsiEI CG_ry'CG
Cuts at: 0 70 1398
Size: 70 1328
BsiHKAI G_wGCw'C
Cuts at: 0 76 404 1398
Size: 76 328 994
Fragments arranged by size:
994 328 76
BsiWI C'GTAC_G
Cuts at: 0 813 1398
Size: 813 585
BsmBI CGTCTCn'nnnn_
Cuts at: 0 644 1398
Size: 644 754
Bsp1286I G_dGCh'C
Cuts at: 0 58 76 224 404 1398
Size: 58 18 148 180 994
Fragments arranged by size:
994 180 148 58 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 468 857 1163 1398
Size: 468 389 306 235
Fragments arranged by size:
468 389 306 235
BsrFI r'CCGG_y
Cuts at: 0 65 473 1398
Size: 65 408 925
Fragments arranged by size:
925 408 65
BssHII G'CGCG_C
Cuts at: 0 125 1398
Size: 125 1273
BstYI r'GATC_y
Cuts at: 0 386 1398
Size: 386 1012
Bsu36I CC'TnA_GG
Cuts at: 0 1267 1398
Size: 1267 131
ClaI AT'CG_AT
Cuts at: 0 767 1398
Size: 767 631
EaeI y'GGCC_r
Cuts at: 0 67 263 395 533 1086 1398
Size: 67 196 132 138 553 312
Fragments arranged by size:
553 312 196 138 132 67
EagI C'GGCC_G
Cuts at: 0 67 1398
Size: 67 1331
EarI CTCTTCn'nnn_
Cuts at: 0 680 710 1037 1247 1398
Size: 680 30 327 210 151
Fragments arranged by size:
680 327 210 151 30
EcoICRI GAG'CTC
Cuts at: 0 74 402 1398
Size: 74 328 996
Fragments arranged by size:
996 328 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 391 565 1398
Size: 60 331 174 833
Fragments arranged by size:
833 331 174 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 33 448 535 853 895 928 1398
Size: 33 415 87 318 42 33 470
Fragments arranged by size:
470 415 318 87 42 33 33
EcoO109I rG'GnC_Cy
Cuts at: 0 441 1398
Size: 441 957
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 1398
Size: 381 1017
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 1294 1326 1346 1378 1398
Size: 622 32 640 32 20 32 20
Fragments arranged by size:
640 622 32 32 32 20 20
HincII GTy'rAC
Cuts at: 0 630 1398
Size: 630 768
MslI CAynn'nnrTG
Cuts at: 0 1139 1385 1398
Size: 1139 246 13
Fragments arranged by size:
1139 246 13
NaeI GCC'GGC
Cuts at: 0 67 475 1398
Size: 67 408 923
Fragments arranged by size:
923 408 67
NgoMIV G'CCGG_C
Cuts at: 0 65 473 1398
Size: 65 408 925
Fragments arranged by size:
925 408 65
NspI r_CATG'y
Cuts at: 0 760 1398
Size: 760 638
PfoI T'CCnGG_A
Cuts at: 0 368 554 725 1398
Size: 368 186 171 673
Fragments arranged by size:
673 368 186 171
PpuMI rG'GwC_Cy
Cuts at: 0 441 1398
Size: 441 957
PshAI GACnn'nnGTC
Cuts at: 0 815 1398
Size: 815 583
PspXI vC'TCGA_Gb
Cuts at: 0 403 1398
Size: 403 995
PstI C_TGCA'G
Cuts at: 0 242 323 737 854 1097 1398
Size: 242 81 414 117 243 301
Fragments arranged by size:
414 301 243 242 117 81
SacI G_AGCT'C
Cuts at: 0 76 404 1398
Size: 76 328 994
Fragments arranged by size:
994 328 76
SbfI CC_TGCA'GG
Cuts at: 0 242 854 1097 1398
Size: 242 612 243 301
Fragments arranged by size:
612 301 243 242
SexAI A'CCwGG_T
Cuts at: 0 7 1398
Size: 7 1391
SfcI C'TryA_G
Cuts at: 0 111 238 319 733 850 1093 1197 1398
Size: 111 127 81 414 117 243 104 201
Fragments arranged by size:
414 243 201 127 117 111 104 81
SmaI CCC'GGG
Cuts at: 0 53 170 225 1398
Size: 53 117 55 1173
Fragments arranged by size:
1173 117 55 53
SmlI C'TyrA_G
Cuts at: 0 403 412 1398
Size: 403 9 986
Fragments arranged by size:
986 403 9
SrfI GCCC'GGGC
Cuts at: 0 53 1398
Size: 53 1345
StyI C'CwwG_G
Cuts at: 0 420 1398
Size: 420 978
Tth111I GACn'n_nGTC
Cuts at: 0 96 1398
Size: 96 1302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 309 1014 1081 1398
Size: 309 705 67 317
Fragments arranged by size:
705 317 309 67
XhoI C'TCGA_G
Cuts at: 0 403 1398
Size: 403 995
XmaI C'CCGG_G
Cuts at: 0 51 168 223 1398
Size: 51 117 55 1175
Fragments arranged by size:
1175 117 55 51
Enzymes that do cut:
AarI AcuI AhdI AleI AlfI AloI AlwNI AvaI
BanI BanII BcgI Bme1580I BmgBI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspMI BsrFI BssHII BstYI Bsu36I
ClaI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII MslI NaeI NgoMIV NspI PfoI
PpuMI PshAI PspXI PstI SacI SbfI SexAI SfcI
SmaI SmlI SrfI StyI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EciI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspOMI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TaqII TatI
TstI XbaI XmnI ZraI