Restriction map for
pFN21AE1920
(Linear) (Six-base) MAPSORT of: KIEE1920.seq Check: 953 from: 1 to: 1365
KIEE1920.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 760 956 1365
Size: 34 726 196 409
Fragments arranged by size:
726 409 196 34
AleI CACnn'nnGTG
Cuts at: 0 379 841 1365
Size: 379 462 524
Fragments arranged by size:
524 462 379
AlwNI CAG_nnn'CTG
Cuts at: 0 102 426 1365
Size: 102 324 939
Fragments arranged by size:
939 324 102
ApaI G_GGCC'C
Cuts at: 0 1099 1365
Size: 1099 266
AvaI C'yCGr_G
Cuts at: 0 59 664 803 1365
Size: 59 605 139 562
Fragments arranged by size:
605 562 139 59
BamHI G'GATC_C
Cuts at: 0 123 131 1365
Size: 123 8 1234
Fragments arranged by size:
1234 123 8
BanII G_rGCy'C
Cuts at: 0 1099 1365
Size: 1099 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 1365
Size: 293 1072
BbvCI CC'TCA_GC
Cuts at: 0 824 1210 1365
Size: 824 386 155
Fragments arranged by size:
824 386 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1091 1125 1365
Size: 1091 34 240
Fragments arranged by size:
1091 240 34
BglI GCCn_nnn'nGGC
Cuts at: 0 898 1365
Size: 898 467
BlpI GC'TnA_GC
Cuts at: 0 332 1365
Size: 332 1033
Bme1580I G_kGCm'C
Cuts at: 0 1099 1365
Size: 1099 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 948 1081 1365
Size: 312 636 133 284
Fragments arranged by size:
636 312 284 133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 347 423 774 1365
Size: 347 76 351 591
Fragments arranged by size:
591 351 347 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 166 451 610 769 771 1365
Size: 124 42 285 159 159 2 594
Fragments arranged by size:
594 285 159 159 124 42 2
Bpu10I CC'TnA_GC
Cuts at: 0 824 869 1210 1365
Size: 824 45 341 155
Fragments arranged by size:
824 341 155 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 586 625 1365
Size: 217 369 39 740
Fragments arranged by size:
740 369 217 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 394 1365
Size: 394 971
BsaWI w'CCGG_w
Cuts at: 0 1286 1365
Size: 1286 79
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 88 91 121 1113 1163 1365
Size: 46 42 3 30 992 50 202
Fragments arranged by size:
992 202 50 46 42 30 3
BseYI C'CCAG_C
Cuts at: 0 1048 1365
Size: 1048 317
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1365
Size: 679 686
BsiHKAI G_wGCw'C
Cuts at: 0 386 1315 1365
Size: 386 929 50
Fragments arranged by size:
929 386 50
BsmI GAATG_Cn'
Cuts at: 0 1114 1365
Size: 1114 251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 538 1365
Size: 538 827
Bsp1286I G_dGCh'C
Cuts at: 0 386 1099 1315 1365
Size: 386 713 216 50
Fragments arranged by size:
713 386 216 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 1365
Size: 701 664
BsrBI CCG'CTC
Cuts at: 0 26 221 777 1365
Size: 26 195 556 588
Fragments arranged by size:
588 556 195 26
BssSI C'ACGA_G
Cuts at: 0 772 1365
Size: 772 593
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 471 1365
Size: 426 45 894
Fragments arranged by size:
894 426 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 795 888 1365
Size: 411 384 93 477
Fragments arranged by size:
477 411 384 93
BstYI r'GATC_y
Cuts at: 0 123 131 767 849 1041 1365
Size: 123 8 636 82 192 324
Fragments arranged by size:
636 324 192 123 82 8
Bsu36I CC'TnA_GG
Cuts at: 0 1265 1365
Size: 1265 100
BtgI C'CryG_G
Cuts at: 0 164 569 794 1365
Size: 164 405 225 571
Fragments arranged by size:
571 405 225 164
BtsI GCAGTG_nn'
Cuts at: 0 1227 1233 1365
Size: 1227 6 132
Fragments arranged by size:
1227 132 6
EaeI y'GGCC_r
Cuts at: 0 413 1365
Size: 413 952
EarI CTCTTCn'nnn_
Cuts at: 0 65 77 110 1317 1365
Size: 65 12 33 1207 48
Fragments arranged by size:
1207 65 48 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 447 520 1365
Size: 447 73 845
Fragments arranged by size:
845 447 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 124 166 451 610 760 769 771
Size: 34 90 42 285 159 150 9 2
Cuts at: 771 956 1365
Size: 185 409
Fragments arranged by size:
409 285 185 159 150 90 42 34
9 2
EcoO109I rG'GnC_Cy
Cuts at: 0 799 1096 1118 1206 1365
Size: 799 297 22 88 159
Fragments arranged by size:
799 297 159 88 22
EcoRV GAT'ATC
Cuts at: 0 174 1365
Size: 174 1191
HaeII r_GCGC'y
Cuts at: 0 786 1365
Size: 786 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 494 1365
Size: 462 32 871
Fragments arranged by size:
871 462 32
MslI CAynn'nnrTG
Cuts at: 0 379 841 886 1348 1365
Size: 379 462 45 462 17
Fragments arranged by size:
462 462 379 45 17
MspA1I CmG'CkG
Cuts at: 0 336 423 588 917 1115 1365
Size: 336 87 165 329 198 250
Fragments arranged by size:
336 329 250 198 165 87
NcoI C'CATG_G
Cuts at: 0 569 794 1365
Size: 569 225 571
Fragments arranged by size:
571 569 225
NspI r_CATG'y
Cuts at: 0 582 1365
Size: 582 783
PflMI CCAn_nnn'nTGG
Cuts at: 0 1278 1365
Size: 1278 87
PspOMI G'GGCC_C
Cuts at: 0 1095 1365
Size: 1095 270
PspXI vC'TCGA_Gb
Cuts at: 0 664 1365
Size: 664 701
PstI C_TGCA'G
Cuts at: 0 698 1365
Size: 698 667
PvuII CAG'CTG
Cuts at: 0 336 588 917 1365
Size: 336 252 329 448
Fragments arranged by size:
448 336 329 252
SbfI CC_TGCA'GG
Cuts at: 0 698 1365
Size: 698 667
ScaI AGT'ACT
Cuts at: 0 652 1365
Size: 652 713
SfcI C'TryA_G
Cuts at: 0 694 918 1306 1365
Size: 694 224 388 59
Fragments arranged by size:
694 388 224 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 898 1365
Size: 898 467
SmaI CCC'GGG
Cuts at: 0 61 1365
Size: 61 1304
SmlI C'TyrA_G
Cuts at: 0 196 601 640 664 1365
Size: 196 405 39 24 701
Fragments arranged by size:
701 405 196 39 24
SphI G_CATG'C
Cuts at: 0 582 1365
Size: 582 783
StuI AGG'CCT
Cuts at: 0 901 1029 1276 1365
Size: 901 128 247 89
Fragments arranged by size:
901 247 128 89
StyI C'CwwG_G
Cuts at: 0 395 569 794 1277 1365
Size: 395 174 225 483 88
Fragments arranged by size:
483 395 225 174 88
TatI w'GTAC_w
Cuts at: 0 650 725 1365
Size: 650 75 640
Fragments arranged by size:
650 640 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 708 1365
Size: 528 180 657
Fragments arranged by size:
657 528 180
XhoI C'TCGA_G
Cuts at: 0 664 1365
Size: 664 701
XmaI C'CCGG_G
Cuts at: 0 59 1365
Size: 59 1306
Enzymes that do cut:
AcuI AleI AlwNI ApaI AvaI BamHI BanII BbsI
BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI
EcoO109I EcoRV HaeII Hin4I MslI MspA1I NcoI NspI
PflMI PspOMI PspXI PstI PvuII SbfI ScaI SfcI
SfiI SmaI SmlI SphI StuI StyI TatI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BciVI BclI BfrBI
BglII BmgBI BmtI BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SexAI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XmnI ZraI