Restriction map for
pFN21AE1918
(Linear) (Six-base) MAPSORT of: KIEE1918.seq Check: 97 from: 1 to: 1362
KIEE1918.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1011 1114 1362
Size: 725 286 103 248
Fragments arranged by size:
725 286 248 103
AflIII A'CryG_T
Cuts at: 0 695 1362
Size: 695 667
ApaI G_GGCC'C
Cuts at: 0 1000 1362
Size: 1000 362
ApaLI G'TGCA_C
Cuts at: 0 1350 1362
Size: 1350 12
ApoI r'AATT_y
Cuts at: 0 16 610 705 1362
Size: 16 594 95 657
Fragments arranged by size:
657 594 95 16
AvrII C'CTAG_G
Cuts at: 0 229 1362
Size: 229 1133
BanII G_rGCy'C
Cuts at: 0 579 1000 1064 1301 1362
Size: 579 421 64 237 61
Fragments arranged by size:
579 421 237 64 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 1362
Size: 545 817
BbvCI CC'TCA_GC
Cuts at: 0 920 1362
Size: 920 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 549 583 1362
Size: 549 34 779
Fragments arranged by size:
779 549 34
BglII A'GATC_T
Cuts at: 0 931 1362
Size: 931 431
Bme1580I G_kGCm'C
Cuts at: 0 1000 1354 1362
Size: 1000 354 8
Fragments arranged by size:
1000 354 8
BmgBI CAC'GTC
Cuts at: 0 7 1362
Size: 7 1355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 1362
Size: 196 1166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 401 751 1172 1362
Size: 231 170 350 421 190
Fragments arranged by size:
421 350 231 190 170
Bpu10I CC'TnA_GC
Cuts at: 0 815 920 1362
Size: 815 105 442
Fragments arranged by size:
815 442 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1299 1362
Size: 445 854 63
Fragments arranged by size:
854 445 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 377 1362
Size: 377 985
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 374 404 535 565 1362
Size: 374 30 131 30 797
Fragments arranged by size:
797 374 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1224 1362
Size: 1224 138
BseYI C'CCAG_C
Cuts at: 0 999 1362
Size: 999 363
BsiHKAI G_wGCw'C
Cuts at: 0 579 816 1064 1354 1362
Size: 579 237 248 290 8
Fragments arranged by size:
579 290 248 237 8
Bsp1286I G_dGCh'C
Cuts at: 0 579 816 1000 1064 1301 1354 1362
Size: 579 237 184 64 237 53 8
Fragments arranged by size:
579 237 237 184 64 53 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1269 1362
Size: 1269 93
BsrGI T'GTAC_A
Cuts at: 0 476 1362
Size: 476 886
BstEII G'GTnAC_C
Cuts at: 0 224 1362
Size: 224 1138
BstYI r'GATC_y
Cuts at: 0 931 1362
Size: 931 431
BtgI C'CryG_G
Cuts at: 0 1199 1362
Size: 1199 163
DrdI GACnn_nn'nnGTC
Cuts at: 0 774 1362
Size: 774 588
EarI CTCTTCn'nnn_
Cuts at: 0 352 905 1362
Size: 352 553 457
Fragments arranged by size:
553 457 352
EcoICRI GAG'CTC
Cuts at: 0 577 1062 1362
Size: 577 485 300
Fragments arranged by size:
577 485 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 401 725 751 1011 1114 1172 1362
Size: 231 170 324 26 260 103 58 190
Fragments arranged by size:
324 260 231 190 170 103 58 26
EcoO109I rG'GnC_Cy
Cuts at: 0 868 1362
Size: 868 494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 486 875 1008 1362
Size: 486 389 133 354
Fragments arranged by size:
486 389 354 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 535 567 916 948 1101 1133
Size: 106 32 397 32 349 32 153 32
Cuts at: 1133 1362
Size: 229
Fragments arranged by size:
397 349 229 153 106 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 1310 1362
Size: 1310 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 701 1149 1362
Size: 98 603 448 213
Fragments arranged by size:
603 448 213 98
MslI CAynn'nnrTG
Cuts at: 0 158 1178 1362
Size: 158 1020 184
Fragments arranged by size:
1020 184 158
MspA1I CmG'CkG
Cuts at: 0 729 830 1092 1362
Size: 729 101 262 270
Fragments arranged by size:
729 270 262 101
NcoI C'CATG_G
Cuts at: 0 1199 1362
Size: 1199 163
NdeI CA'TA_TG
Cuts at: 0 285 1362
Size: 285 1077
NspI r_CATG'y
Cuts at: 0 699 1362
Size: 699 663
PasI CC'CwG_GG
Cuts at: 0 864 1362
Size: 864 498
PciI A'CATG_T
Cuts at: 0 695 1362
Size: 695 667
PfoI T'CCnGG_A
Cuts at: 0 378 1362
Size: 378 984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 443 475 1362
Size: 443 32 887
Fragments arranged by size:
887 443 32
PpuMI rG'GwC_Cy
Cuts at: 0 868 1362
Size: 868 494
PspOMI G'GGCC_C
Cuts at: 0 996 1362
Size: 996 366
PstI C_TGCA'G
Cuts at: 0 152 239 1362
Size: 152 87 1123
Fragments arranged by size:
1123 152 87
PvuII CAG'CTG
Cuts at: 0 729 830 1092 1362
Size: 729 101 262 270
Fragments arranged by size:
729 270 262 101
SacI G_AGCT'C
Cuts at: 0 579 1064 1362
Size: 579 485 298
Fragments arranged by size:
579 485 298
SapI GCTCTTCn'nnn_
Cuts at: 0 352 1362
Size: 352 1010
SexAI A'CCwGG_T
Cuts at: 0 386 1362
Size: 386 976
SfcI C'TryA_G
Cuts at: 0 148 235 1362
Size: 148 87 1127
Fragments arranged by size:
1127 148 87
SmlI C'TyrA_G
Cuts at: 0 460 1278 1362
Size: 460 818 84
Fragments arranged by size:
818 460 84
StuI AGG'CCT
Cuts at: 0 858 1362
Size: 858 504
StyI C'CwwG_G
Cuts at: 0 66 136 229 293 992 1159 1199 1362
Size: 66 70 93 64 699 167 40 163
Fragments arranged by size:
699 167 163 93 70 66 64 40
TatI w'GTAC_w
Cuts at: 0 476 1362
Size: 476 886
XmnI GAAnn'nnTTC
Cuts at: 0 302 1362
Size: 302 1060
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI AvrII BanII BbsI
BbvCI BcgI BglII Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrGI BstEII BstYI BtgI DrdI EarI EcoICRI Eco57MI
EcoO109I FalI Hin4I MfeI MmeI MslI MspA1I NcoI
NdeI NspI PasI PciI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SapI SexAI SfcI SmlI StuI
StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvaI BaeI BamHI BanI BbeI BciVI BclI BfrBI
BglI BlpI BmrI BmtI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI