Restriction map for
pFN21AE1916
(Linear) (Six-base) MAPSORT of: KIEE1916.seq Check: 6822 from: 1 to: 1359
KIEE1916.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AccI GT'mk_AC
Cuts at: 0 355 746 1359
Size: 355 391 613
Fragments arranged by size:
613 391 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1098 1359
Size: 854 244 261
Fragments arranged by size:
854 261 244
AflIII A'CryG_T
Cuts at: 0 737 1359
Size: 737 622
AhdI GACnn_n'nnGTC
Cuts at: 0 933 1359
Size: 933 426
ApoI r'AATT_y
Cuts at: 0 527 558 892 1359
Size: 527 31 334 467
Fragments arranged by size:
527 467 334 31
AseI AT'TA_AT
Cuts at: 0 305 617 1359
Size: 305 312 742
Fragments arranged by size:
742 312 305
AvaI C'yCGr_G
Cuts at: 0 1161 1359
Size: 1161 198
BanI G'GyrC_C
Cuts at: 0 1289 1359
Size: 1289 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 447 1359
Size: 447 912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 723 757 1359
Size: 723 34 602
Fragments arranged by size:
723 602 34
BclI T'GATC_A
Cuts at: 0 579 1359
Size: 579 780
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1359
Size: 32 1327
BglII A'GATC_T
Cuts at: 0 138 1359
Size: 138 1221
BmgBI CAC'GTC
Cuts at: 0 90 1359
Size: 90 1269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1174 1359
Size: 155 1019 185
Fragments arranged by size:
1019 185 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 443 973 1359
Size: 443 530 386
Fragments arranged by size:
530 443 386
BsaWI w'CCGG_w
Cuts at: 0 261 1359
Size: 261 1098
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 796 802 826 1359
Size: 772 24 6 24 533
Fragments arranged by size:
772 533 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 780 1359
Size: 700 80 579
Fragments arranged by size:
700 579 80
BseYI C'CCAG_C
Cuts at: 0 841 1359
Size: 841 518
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1359
Size: 1261 98
BsmI GAATG_Cn'
Cuts at: 0 339 1359
Size: 339 1020
BspHI T'CATG_A
Cuts at: 0 255 582 1088 1359
Size: 255 327 506 271
Fragments arranged by size:
506 327 271 255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 1010 1359
Size: 54 956 349
Fragments arranged by size:
956 349 54
BsrDI GCAATG_nn'
Cuts at: 0 1307 1359
Size: 1307 52
BsrFI r'CCGG_y
Cuts at: 0 972 1359
Size: 972 387
BsrGI T'GTAC_A
Cuts at: 0 1334 1359
Size: 1334 25
BssHII G'CGCG_C
Cuts at: 0 1182 1359
Size: 1182 177
BssSI C'ACGA_G
Cuts at: 0 994 1359
Size: 994 365
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1016 1359
Size: 1016 343
BstEII G'GTnAC_C
Cuts at: 0 320 1359
Size: 320 1039
BstYI r'GATC_y
Cuts at: 0 138 498 837 1271 1359
Size: 138 360 339 434 88
Fragments arranged by size:
434 360 339 138 88
BtgI C'CryG_G
Cuts at: 0 29 494 520 1295 1313 1359
Size: 29 465 26 775 18 46
Fragments arranged by size:
775 465 46 29 26 18
BtsI GCAGTG_nn'
Cuts at: 0 704 1359
Size: 704 655
DraIII CAC_nnn'GTG
Cuts at: 0 1296 1359
Size: 1296 63
EaeI y'GGCC_r
Cuts at: 0 92 1359
Size: 92 1267
EarI CTCTTCn'nnn_
Cuts at: 0 821 1359
Size: 821 538
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 1359
Size: 760 599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 854 1098 1174 1359
Size: 155 699 244 76 185
Fragments arranged by size:
699 244 185 155 76
EcoO109I rG'GnC_Cy
Cuts at: 0 477 851 951 1097 1359
Size: 477 374 100 146 262
Fragments arranged by size:
477 374 262 146 100
FspI TGC'GCA
Cuts at: 0 1006 1359
Size: 1006 353
HaeII r_GCGC'y
Cuts at: 0 472 1085 1359
Size: 472 613 274
Fragments arranged by size:
613 472 274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 796 804 828 919 951 1359
Size: 772 24 8 24 91 32 408
Fragments arranged by size:
772 408 91 32 24 24 8
HindIII A'AGCT_T
Cuts at: 0 648 1359
Size: 648 711
MslI CAynn'nnrTG
Cuts at: 0 1312 1359
Size: 1312 47
MspA1I CmG'CkG
Cuts at: 0 31 1359
Size: 31 1328
NcoI C'CATG_G
Cuts at: 0 494 1359
Size: 494 865
NspI r_CATG'y
Cuts at: 0 786 1359
Size: 786 573
PfoI T'CCnGG_A
Cuts at: 0 1343 1359
Size: 1343 16
PpuMI rG'GwC_Cy
Cuts at: 0 477 851 951 1097 1359
Size: 477 374 100 146 262
Fragments arranged by size:
477 374 262 146 100
PshAI GACnn'nnGTC
Cuts at: 0 697 1359
Size: 697 662
PsiI TTA'TAA
Cuts at: 0 419 1359
Size: 419 940
PstI C_TGCA'G
Cuts at: 0 603 711 1359
Size: 603 108 648
Fragments arranged by size:
648 603 108
SacII CC_GC'GG
Cuts at: 0 32 1359
Size: 32 1327
SanDI GG'GwC_CC
Cuts at: 0 477 1359
Size: 477 882
SapI GCTCTTCn'nnn_
Cuts at: 0 821 1359
Size: 821 538
SexAI A'CCwGG_T
Cuts at: 0 986 1359
Size: 986 373
SfcI C'TryA_G
Cuts at: 0 599 707 747 1359
Size: 599 108 40 612
Fragments arranged by size:
612 599 108 40
SmlI C'TyrA_G
Cuts at: 0 1161 1359
Size: 1161 198
SspI AAT'ATT
Cuts at: 0 485 1359
Size: 485 874
StuI AGG'CCT
Cuts at: 0 1159 1359
Size: 1159 200
StyI C'CwwG_G
Cuts at: 0 494 1359
Size: 494 865
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1359
Size: 709 650
TatI w'GTAC_w
Cuts at: 0 1334 1359
Size: 1334 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 1359
Size: 375 32 952
Fragments arranged by size:
952 375 32
Tth111I GACn'n_nGTC
Cuts at: 0 1095 1303 1359
Size: 1095 208 56
Fragments arranged by size:
1095 208 56
XbaI T'CTAG_A
Cuts at: 0 285 1359
Size: 285 1074
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 1359
Size: 501 858
XhoI C'TCGA_G
Cuts at: 0 1161 1359
Size: 1161 198
Enzymes that do cut:
AccI AcuI AflIII AhdI ApoI AseI AvaI BanI
BbsI BcgI BclI BglI BglII BmgBI BpmI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsmI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstYI
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I
FspI HaeII Hin4I HindIII MslI MspA1I NcoI NspI
PfoI PpuMI PshAI PsiI PstI SacII SanDI SapI
SexAI SfcI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BciVI BfrBI BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI XmaI XmnI ZraI