Restriction map for
pFN21AE1914
(Linear) (Six-base) MAPSORT of: KIEE1914.seq Check: 191 from: 1 to: 1353
KIEE1914.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 664 1353
Size: 483 181 689
Fragments arranged by size:
689 483 181
AleI CACnn'nnGTG
Cuts at: 0 368 535 1353
Size: 368 167 818
Fragments arranged by size:
818 368 167
AlwNI CAG_nnn'CTG
Cuts at: 0 987 1016 1353
Size: 987 29 337
Fragments arranged by size:
987 337 29
ApaLI G'TGCA_C
Cuts at: 0 1183 1353
Size: 1183 170
ApoI r'AATT_y
Cuts at: 0 492 1219 1353
Size: 492 727 134
Fragments arranged by size:
727 492 134
AvaI C'yCGr_G
Cuts at: 0 182 726 1073 1353
Size: 182 544 347 280
Fragments arranged by size:
544 347 280 182
BanII G_rGCy'C
Cuts at: 0 249 545 849 866 1353
Size: 249 296 304 17 487
Fragments arranged by size:
487 304 296 249 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1353
Size: 27 34 1292
Fragments arranged by size:
1292 34 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 1266 1353
Size: 1266 87
BglI GCCn_nnn'nGGC
Cuts at: 0 35 392 1077 1353
Size: 35 357 685 276
Fragments arranged by size:
685 357 276 35
BglII A'GATC_T
Cuts at: 0 1127 1353
Size: 1127 226
BlpI GC'TnA_GC
Cuts at: 0 1081 1353
Size: 1081 272
Bme1580I G_kGCm'C
Cuts at: 0 182 427 445 1187 1353
Size: 182 245 18 742 166
Fragments arranged by size:
742 245 182 166 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1353
Size: 309 1044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1170 1353
Size: 1170 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1353
Size: 151 1202
BsaI GGTCTCn'nnnn_
Cuts at: 0 1146 1353
Size: 1146 207
BsaWI w'CCGG_w
Cuts at: 0 482 719 1353
Size: 482 237 634
Fragments arranged by size:
634 482 237
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1333 1353
Size: 484 849 20
Fragments arranged by size:
849 484 20
BseYI C'CCAG_C
Cuts at: 0 97 869 1353
Size: 97 772 484
Fragments arranged by size:
772 484 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1018 1353
Size: 772 246 335
Fragments arranged by size:
772 335 246
BsiEI CG_ry'CG
Cuts at: 0 108 1353
Size: 108 1245
BsiHKAI G_wGCw'C
Cuts at: 0 249 866 1187 1353
Size: 249 617 321 166
Fragments arranged by size:
617 321 249 166
BsmI GAATG_Cn'
Cuts at: 0 224 588 1353
Size: 224 364 765
Fragments arranged by size:
765 364 224
Bsp1286I G_dGCh'C
Cuts at: 0 182 249 427 445 545 849 866 1187
Size: 182 67 178 18 100 304 17 321
Cuts at: 1187 1353
Size: 166
Fragments arranged by size:
321 304 182 178 166 100 67 18
17
BspHI T'CATG_A
Cuts at: 0 452 488 1353
Size: 452 36 865
Fragments arranged by size:
865 452 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1353
Size: 755 598
BsrBI CCG'CTC
Cuts at: 0 701 1353
Size: 701 652
BssSI C'ACGA_G
Cuts at: 0 364 1353
Size: 364 989
BstXI CCAn_nnnn'nTGG
Cuts at: 0 975 1353
Size: 975 378
BstYI r'GATC_y
Cuts at: 0 669 1127 1353
Size: 669 458 226
Fragments arranged by size:
669 458 226
BtgI C'CryG_G
Cuts at: 0 108 273 854 896 918 1353
Size: 108 165 581 42 22 435
Fragments arranged by size:
581 435 165 108 42 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1083 1118 1353
Size: 1083 35 235
Fragments arranged by size:
1083 235 35
DraIII CAC_nnn'GTG
Cuts at: 0 684 1353
Size: 684 669
DrdI GACnn_nn'nnGTC
Cuts at: 0 268 1353
Size: 268 1085
EaeI y'GGCC_r
Cuts at: 0 27 857 921 1088 1115 1353
Size: 27 830 64 167 27 238
Fragments arranged by size:
830 238 167 64 27 27
EarI CTCTTCn'nnn_
Cuts at: 0 1301 1353
Size: 1301 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 904 1353
Size: 904 449
EcoICRI GAG'CTC
Cuts at: 0 247 864 1353
Size: 247 617 489
Fragments arranged by size:
617 489 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 483 664 1353
Size: 151 332 181 689
Fragments arranged by size:
689 332 181 151
EcoNI CCTnn'n_nnAGG
Cuts at: 0 165 1353
Size: 165 1188
EcoO109I rG'GnC_Cy
Cuts at: 0 113 615 1353
Size: 113 502 738
Fragments arranged by size:
738 502 113
EcoRI G'AATT_C
Cuts at: 0 492 1353
Size: 492 861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1193 1353
Size: 1193 160
FspI TGC'GCA
Cuts at: 0 751 1353
Size: 751 602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 429 461 1240 1272 1353
Size: 429 32 779 32 81
Fragments arranged by size:
779 429 81 32 32
HindIII A'AGCT_T
Cuts at: 0 1175 1353
Size: 1175 178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1353
Size: 506 847
MscI TGG'CCA
Cuts at: 0 29 1353
Size: 29 1324
MslI CAynn'nnrTG
Cuts at: 0 368 535 1353
Size: 368 167 818
Fragments arranged by size:
818 368 167
MspA1I CmG'CkG
Cuts at: 0 110 129 573 1016 1353
Size: 110 19 444 443 337
Fragments arranged by size:
444 443 337 110 19
NcoI C'CATG_G
Cuts at: 0 273 896 1353
Size: 273 623 457
Fragments arranged by size:
623 457 273
PasI CC'CwG_GG
Cuts at: 0 414 825 1353
Size: 414 411 528
Fragments arranged by size:
528 414 411
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 1251 1353
Size: 59 355 837 102
Fragments arranged by size:
837 355 102 59
PfoI T'CCnGG_A
Cuts at: 0 433 1353
Size: 433 920
PstI C_TGCA'G
Cuts at: 0 392 1353
Size: 392 961
PvuII CAG'CTG
Cuts at: 0 129 573 1016 1353
Size: 129 444 443 337
Fragments arranged by size:
444 443 337 129
RsrII CG'GwC_CG
Cuts at: 0 480 1353
Size: 480 873
SacI G_AGCT'C
Cuts at: 0 249 866 1353
Size: 249 617 487
Fragments arranged by size:
617 487 249
SacII CC_GC'GG
Cuts at: 0 111 1353
Size: 111 1242
SbfI CC_TGCA'GG
Cuts at: 0 392 1353
Size: 392 961
SfcI C'TryA_G
Cuts at: 0 388 1353
Size: 388 965
SmaI CCC'GGG
Cuts at: 0 728 1075 1353
Size: 728 347 278
Fragments arranged by size:
728 347 278
StyI C'CwwG_G
Cuts at: 0 273 518 896 968 1353
Size: 273 245 378 72 385
Fragments arranged by size:
385 378 273 245 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 672 883 1353
Size: 672 211 470
Fragments arranged by size:
672 470 211
TatI w'GTAC_w
Cuts at: 0 146 1353
Size: 146 1207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 1353
Size: 670 32 651
Fragments arranged by size:
670 651 32
XbaI T'CTAG_A
Cuts at: 0 1293 1353
Size: 1293 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 22 280 972 1203 1353
Size: 22 258 692 231 150
Fragments arranged by size:
692 258 231 150 22
XmaI C'CCGG_G
Cuts at: 0 726 1073 1353
Size: 726 347 280
Fragments arranged by size:
726 347 280
XmnI GAAnn'nnTTC
Cuts at: 0 496 1353
Size: 496 857
Enzymes that do cut:
AcuI AleI AlwNI ApaLI ApoI AvaI BanII BcgI
BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BssSI BstXI BstYI BtgI
CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI Hin4I HindIII MmeI
MscI MslI MspA1I NcoI PasI PflMI PfoI PstI
PvuII RsrII SacI SacII SbfI SfcI SmaI StyI
TaqII TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BclI
BfrBI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BsiWI BsmBI BspEI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
DraI EagI EcoRV FseI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XhoI ZraI