Restriction map for
pFN21AE1910
(Linear) (Six-base) MAPSORT of: KIEE1910.seq Check: 9557 from: 1 to: 1332
KIEE1910.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 26 721 1332
Size: 26 695 611
Fragments arranged by size:
695 611 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 483 1102 1332
Size: 373 110 619 230
Fragments arranged by size:
619 373 230 110
AflIII A'CryG_T
Cuts at: 0 143 1332
Size: 143 1189
AleI CACnn'nnGTG
Cuts at: 0 535 1332
Size: 535 797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1285 1317 1332
Size: 1285 32 15
Fragments arranged by size:
1285 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 990 1128 1332
Size: 990 138 204
Fragments arranged by size:
990 204 138
ApaI G_GGCC'C
Cuts at: 0 849 1332
Size: 849 483
ApaLI G'TGCA_C
Cuts at: 0 13 565 1332
Size: 13 552 767
Fragments arranged by size:
767 552 13
AvaI C'yCGr_G
Cuts at: 0 726 859 1332
Size: 726 133 473
Fragments arranged by size:
726 473 133
BamHI G'GATC_C
Cuts at: 0 1031 1332
Size: 1031 301
BanI G'GyrC_C
Cuts at: 0 211 507 855 1332
Size: 211 296 348 477
Fragments arranged by size:
477 348 296 211
BanII G_rGCy'C
Cuts at: 0 545 849 866 1332
Size: 545 304 17 466
Fragments arranged by size:
545 466 304 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1054 1332
Size: 1054 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1002 1036 1332
Size: 27 34 941 34 296
Fragments arranged by size:
941 296 34 34 27
BglI GCCn_nnn'nGGC
Cuts at: 0 35 392 1332
Size: 35 357 940
Fragments arranged by size:
940 357 35
BglII A'GATC_T
Cuts at: 0 248 1332
Size: 248 1084
BlpI GC'TnA_GC
Cuts at: 0 991 1332
Size: 991 341
Bme1580I G_kGCm'C
Cuts at: 0 17 314 445 512 569 849 860 1332
Size: 17 297 131 67 57 280 11 472
Fragments arranged by size:
472 297 280 131 67 57 17 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1332
Size: 309 1023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1332
Size: 151 1181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1332
Size: 628 704
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1332
Size: 586 746
BsaHI Gr'CG_yC
Cuts at: 0 1280 1332
Size: 1280 52
BsaWI w'CCGG_w
Cuts at: 0 283 359 433 1332
Size: 283 76 74 899
Fragments arranged by size:
899 283 76 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1285 1315 1332
Size: 1285 30 17
Fragments arranged by size:
1285 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1306 1321 1324 1332
Size: 484 822 15 3 8
Fragments arranged by size:
822 484 15 8 3
BseYI C'CCAG_C
Cuts at: 0 414 869 1332
Size: 414 455 463
Fragments arranged by size:
463 455 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1018 1332
Size: 772 246 314
Fragments arranged by size:
772 314 246
BsiEI CG_ry'CG
Cuts at: 0 259 924 1055 1289 1332
Size: 259 665 131 234 43
Fragments arranged by size:
665 259 234 131 43
BsiHKAI G_wGCw'C
Cuts at: 0 17 569 866 1332
Size: 17 552 297 466
Fragments arranged by size:
552 466 297 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 314 445 512 545 569 849 860
Size: 17 297 131 67 33 24 280 11
Cuts at: 860 866 1332
Size: 6 466
Fragments arranged by size:
466 297 280 131 67 33 24 17
11 6
BspEI T'CCGG_A
Cuts at: 0 283 359 433 1332
Size: 283 76 74 899
Fragments arranged by size:
899 283 76 74
BspHI T'CATG_A
Cuts at: 0 488 1332
Size: 488 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 131 721 755 1332
Size: 26 105 590 34 577
Fragments arranged by size:
590 577 105 34 26
BsrBI CCG'CTC
Cuts at: 0 187 701 1332
Size: 187 514 631
Fragments arranged by size:
631 514 187
BsrFI r'CCGG_y
Cuts at: 0 25 289 1332
Size: 25 264 1043
Fragments arranged by size:
1043 264 25
BstEII G'GTnAC_C
Cuts at: 0 705 1332
Size: 705 627
BstYI r'GATC_y
Cuts at: 0 248 461 1031 1332
Size: 248 213 570 301
Fragments arranged by size:
570 301 248 213
BtgI C'CryG_G
Cuts at: 0 108 215 918 935 1074 1283 1332
Size: 108 107 703 17 139 209 49
Fragments arranged by size:
703 209 139 108 107 49 17
DraIII CAC_nnn'GTG
Cuts at: 0 684 1332
Size: 684 648
EaeI y'GGCC_r
Cuts at: 0 27 256 276 689 758 921 938 1052
Size: 27 229 20 413 69 163 17 114
Cuts at: 1052 1088 1115 1157 1286 1332
Size: 36 27 42 129 46
Fragments arranged by size:
413 229 163 129 114 69 46 42
36 27 27 20 17
EagI C'GGCC_G
Cuts at: 0 256 921 1052 1286 1332
Size: 256 665 131 234 46
Fragments arranged by size:
665 256 234 131 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1330 1332
Size: 1330 2
EcoICRI GAG'CTC
Cuts at: 0 864 1332
Size: 864 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 373 483 1102 1332
Size: 151 222 110 619 230
Fragments arranged by size:
619 230 222 151 110
EcoO109I rG'GnC_Cy
Cuts at: 0 669 846 1332
Size: 669 177 486
Fragments arranged by size:
669 486 177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1197 1332
Size: 1197 135
FseI GG_CCGG'CC
Cuts at: 0 29 1332
Size: 29 1303
FspI TGC'GCA
Cuts at: 0 751 1332
Size: 751 581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 1332
Size: 1240 32 60
Fragments arranged by size:
1240 60 32
HincII GTy'rAC
Cuts at: 0 765 1332
Size: 765 567
MscI TGG'CCA
Cuts at: 0 278 1332
Size: 278 1054
MslI CAynn'nnrTG
Cuts at: 0 535 964 1159 1332
Size: 535 429 195 173
Fragments arranged by size:
535 429 195 173
MspA1I CmG'CkG
Cuts at: 0 402 573 735 1076 1332
Size: 402 171 162 341 256
Fragments arranged by size:
402 341 256 171 162
NaeI GCC'GGC
Cuts at: 0 27 291 1332
Size: 27 264 1041
Fragments arranged by size:
1041 264 27
NcoI C'CATG_G
Cuts at: 0 108 215 1332
Size: 108 107 1117
Fragments arranged by size:
1117 108 107
NgoMIV G'CCGG_C
Cuts at: 0 25 289 1332
Size: 25 264 1043
Fragments arranged by size:
1043 264 25
NspI r_CATG'y
Cuts at: 0 963 1332
Size: 963 369
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 576 1332
Size: 59 355 162 756
Fragments arranged by size:
756 355 162 59
PfoI T'CCnGG_A
Cuts at: 0 347 1121 1332
Size: 347 774 211
Fragments arranged by size:
774 347 211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1285 1317 1332
Size: 1204 32 49 32 15
Fragments arranged by size:
1204 49 32 32 15
PpuMI rG'GwC_Cy
Cuts at: 0 669 1332
Size: 669 663
PspOMI G'GGCC_C
Cuts at: 0 845 1332
Size: 845 487
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1332
Size: 1000 32 300
Fragments arranged by size:
1000 300 32
PstI C_TGCA'G
Cuts at: 0 23 1332
Size: 23 1309
PvuII CAG'CTG
Cuts at: 0 402 573 735 1332
Size: 402 171 162 597
Fragments arranged by size:
597 402 171 162
SacI G_AGCT'C
Cuts at: 0 866 1332
Size: 866 466
SacII CC_GC'GG
Cuts at: 0 1077 1332
Size: 1077 255
SbfI CC_TGCA'GG
Cuts at: 0 23 1332
Size: 23 1309
SexAI A'CCwGG_T
Cuts at: 0 680 1250 1332
Size: 680 570 82
Fragments arranged by size:
680 570 82
SfcI C'TryA_G
Cuts at: 0 19 1332
Size: 19 1313
SmaI CCC'GGG
Cuts at: 0 728 1332
Size: 728 604
SmlI C'TyrA_G
Cuts at: 0 643 1332
Size: 643 689
StuI AGG'CCT
Cuts at: 0 1177 1332
Size: 1177 155
StyI C'CwwG_G
Cuts at: 0 108 215 1332
Size: 108 107 1117
Fragments arranged by size:
1117 108 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 883 1332
Size: 232 651 449
Fragments arranged by size:
651 449 232
TatI w'GTAC_w
Cuts at: 0 146 1332
Size: 146 1186
Tth111I GACn'n_nGTC
Cuts at: 0 223 343 355 1332
Size: 223 120 12 977
Fragments arranged by size:
977 223 120 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 894 972 1332
Size: 189 705 78 360
Fragments arranged by size:
705 360 189 78
XmaI C'CCGG_G
Cuts at: 0 726 1332
Size: 726 606
XmnI GAAnn'nnTTC
Cuts at: 0 496 1332
Size: 496 836
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BcgI BglI BglII
BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BstEII BstYI BtgI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoO109I FalI FseI
FspI Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI PsrI
PstI PvuII SacI SacII SbfI SexAI SfcI SmaI
SmlI StuI StyI TaqII TatI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BciVI BclI BfrBI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BsiWI BsmI BsmBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EarI EcoNI EcoRI EcoRV
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PshAI PsiI PspXI PvuI
RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI ZraI