Restriction map for
pFN21AE1908
(Linear) (Six-base) MAPSORT of: KIEE1908.seq Check: 8254 from: 1 to: 1245
KIEE1908.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 1245
Size: 166 1079
AgeI A'CCGG_T
Cuts at: 0 1136 1245
Size: 1136 109
AleI CACnn'nnGTG
Cuts at: 0 949 1245
Size: 949 296
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 1245
Size: 460 32 753
Fragments arranged by size:
753 460 32
ApaI G_GGCC'C
Cuts at: 0 823 1245
Size: 823 422
ApaLI G'TGCA_C
Cuts at: 0 340 1245
Size: 340 905
AvaI C'yCGr_G
Cuts at: 0 532 1000 1019 1166 1245
Size: 532 468 19 147 79
Fragments arranged by size:
532 468 147 79 19
BanI G'GyrC_C
Cuts at: 0 8 154 443 1133 1245
Size: 8 146 289 690 112
Fragments arranged by size:
690 289 146 112 8
BanII G_rGCy'C
Cuts at: 0 532 823 1245
Size: 532 291 422
Fragments arranged by size:
532 422 291
BbsI GAAGACnn'nnnn_
Cuts at: 0 1020 1245
Size: 1020 225
BclI T'GATC_A
Cuts at: 0 842 1245
Size: 842 403
BglI GCCn_nnn'nGGC
Cuts at: 0 1171 1245
Size: 1171 74
Bme1580I G_kGCm'C
Cuts at: 0 11 344 716 823 1167 1245
Size: 11 333 372 107 344 78
Fragments arranged by size:
372 344 333 107 78 11
BmgBI CAC'GTC
Cuts at: 0 908 1245
Size: 908 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1245
Size: 58 1187
Bpu10I CC'TnA_GC
Cuts at: 0 430 1245
Size: 430 815
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 1020 1245
Size: 907 113 225
Fragments arranged by size:
907 225 113
BsaBI GATnn'nnATC
Cuts at: 0 691 1245
Size: 691 554
BsaWI w'CCGG_w
Cuts at: 0 440 1121 1136 1245
Size: 440 681 15 109
Fragments arranged by size:
681 440 109 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 460 490 1027 1057 1245
Size: 370 30 60 30 537 30 188
Fragments arranged by size:
537 370 188 60 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 1245
Size: 337 908
BseYI C'CCAG_C
Cuts at: 0 275 704 1245
Size: 275 429 541
Fragments arranged by size:
541 429 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 622 1245
Size: 582 40 623
Fragments arranged by size:
623 582 40
BsiHKAI G_wGCw'C
Cuts at: 0 344 656 1245
Size: 344 312 589
Fragments arranged by size:
589 344 312
BsmBI CGTCTCn'nnnn_
Cuts at: 0 28 66 1245
Size: 28 38 1179
Fragments arranged by size:
1179 38 28
Bsp1286I G_dGCh'C
Cuts at: 0 11 344 532 656 716 823 1167 1245
Size: 11 333 188 124 60 107 344 78
Fragments arranged by size:
344 333 188 124 107 78 60 11
BspEI T'CCGG_A
Cuts at: 0 1121 1245
Size: 1121 124
BspHI T'CATG_A
Cuts at: 0 881 1245
Size: 881 364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 166 554 644 1245
Size: 166 388 90 601
Fragments arranged by size:
601 388 166 90
BsrFI r'CCGG_y
Cuts at: 0 671 1136 1245
Size: 671 465 109
Fragments arranged by size:
671 465 109
BsrGI T'GTAC_A
Cuts at: 0 797 1245
Size: 797 448
BstAPI GCAn_nnn'nTGC
Cuts at: 0 443 1245
Size: 443 802
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 831 1245
Size: 729 102 414
Fragments arranged by size:
729 414 102
BstYI r'GATC_y
Cuts at: 0 311 1245
Size: 311 934
BtgI C'CryG_G
Cuts at: 0 140 1245
Size: 140 1105
BtsI GCAGTG_nn'
Cuts at: 0 84 133 1245
Size: 84 49 1112
Fragments arranged by size:
1112 84 49
EaeI y'GGCC_r
Cuts at: 0 104 263 1245
Size: 104 159 982
Fragments arranged by size:
982 159 104
EarI CTCTTCn'nnn_
Cuts at: 0 1120 1245
Size: 1120 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 468 1245
Size: 468 777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1245
Size: 58 1187
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1245
Size: 319 926
EcoO109I rG'GnC_Cy
Cuts at: 0 33 819 820 1245
Size: 33 786 1 425
Fragments arranged by size:
786 425 33 1
FspI TGC'GCA
Cuts at: 0 493 679 1245
Size: 493 186 566
Fragments arranged by size:
566 493 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 1000 1032 1138 1170 1245
Size: 82 32 886 32 106 32 75
Fragments arranged by size:
886 106 82 75 32 32 32
MscI TGG'CCA
Cuts at: 0 106 265 1245
Size: 106 159 980
Fragments arranged by size:
980 159 106
MslI CAynn'nnrTG
Cuts at: 0 347 727 880 949 979 1245
Size: 347 380 153 69 30 266
Fragments arranged by size:
380 347 266 153 69 30
MspA1I CmG'CkG
Cuts at: 0 275 1245
Size: 275 970
NspI r_CATG'y
Cuts at: 0 984 1245
Size: 984 261
PfoI T'CCnGG_A
Cuts at: 0 74 1049 1245
Size: 74 975 196
Fragments arranged by size:
975 196 74
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 460 492 1245
Size: 46 32 382 32 753
Fragments arranged by size:
753 382 46 32 32
PspOMI G'GGCC_C
Cuts at: 0 819 1245
Size: 819 426
PstI C_TGCA'G
Cuts at: 0 128 1245
Size: 128 1117
PvuII CAG'CTG
Cuts at: 0 275 1245
Size: 275 970
SexAI A'CCwGG_T
Cuts at: 0 502 1245
Size: 502 743
SfcI C'TryA_G
Cuts at: 0 124 1245
Size: 124 1121
SgrAI Cr'CCGG_yG
Cuts at: 0 1136 1245
Size: 1136 109
SmaI CCC'GGG
Cuts at: 0 1002 1021 1245
Size: 1002 19 224
Fragments arranged by size:
1002 224 19
StuI AGG'CCT
Cuts at: 0 789 1245
Size: 789 456
TatI w'GTAC_w
Cuts at: 0 752 797 923 1245
Size: 752 45 126 322
Fragments arranged by size:
752 322 126 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 1245
Size: 370 32 843
Fragments arranged by size:
843 370 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 507 1245
Size: 507 738
XmaI C'CCGG_G
Cuts at: 0 1000 1019 1245
Size: 1000 19 226
Fragments arranged by size:
1000 226 19
Enzymes that do cut:
AarI AgeI AleI AloI ApaI ApaLI AvaI BanI
BanII BbsI BclI BglI Bme1580I BmgBI BpmI Bpu10I
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BstAPI
BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI
EcoNI EcoO109I FspI Hin4I MscI MslI MspA1I NspI
PfoI PpiI PspOMI PstI PvuII SexAI SfcI SgrAI
SmaI StuI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlfI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII
BlpI BmrI BmtI BplI BpuEI BsaAI BsaHI BsiEI
BsiWI BsmI BsrBI BsrDI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI