Restriction map for
pFN21AE1907
(Linear) (Six-base) MAPSORT of: KIEE1907.seq Check: 4020 from: 1 to: 1326
KIEE1907.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AccI GT'mk_AC
Cuts at: 0 146 1326
Size: 146 1180
AfeI AGC'GCT
Cuts at: 0 496 1326
Size: 496 830
AflIII A'CryG_T
Cuts at: 0 410 624 1166 1326
Size: 410 214 542 160
Fragments arranged by size:
542 410 214 160
AleI CACnn'nnGTG
Cuts at: 0 94 1326
Size: 94 1232
ApaI G_GGCC'C
Cuts at: 0 39 377 678 1326
Size: 39 338 301 648
Fragments arranged by size:
648 338 301 39
BanI G'GyrC_C
Cuts at: 0 3 807 1286 1326
Size: 3 804 479 40
Fragments arranged by size:
804 479 40 3
BanII G_rGCy'C
Cuts at: 0 39 67 122 377 678 1000 1326
Size: 39 28 55 255 301 322 326
Fragments arranged by size:
326 322 301 255 55 39 28
BbeI G_GCGC'C
Cuts at: 0 7 1326
Size: 7 1319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1216 1250 1326
Size: 1216 34 76
Fragments arranged by size:
1216 76 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1219 1326
Size: 1219 107
Bme1580I G_kGCm'C
Cuts at: 0 39 377 678 1326
Size: 39 338 301 648
Fragments arranged by size:
648 338 301 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1040 1326
Size: 1040 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 562 577 836 980 1326
Size: 331 231 15 259 144 346
Fragments arranged by size:
346 331 259 231 144 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1326
Size: 96 1230
BsaI GGTCTCn'nnnn_
Cuts at: 0 341 751 1326
Size: 341 410 575
Fragments arranged by size:
575 410 341
BsaAI yAC'GTr
Cuts at: 0 144 1326
Size: 144 1182
BsaHI Gr'CG_yC
Cuts at: 0 4 179 384 1326
Size: 4 175 205 942
Fragments arranged by size:
942 205 175 4
BsaWI w'CCGG_w
Cuts at: 0 1127 1326
Size: 1127 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 374 404 439 469 535 565 938 953
Size: 374 30 35 30 66 30 373 15
Cuts at: 953 968 983 1122 1152 1326
Size: 15 15 139 30 174
Fragments arranged by size:
374 373 174 139 66 35 30 30
30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 379 538 1326
Size: 7 372 159 788
Fragments arranged by size:
788 372 159 7
BseYI C'CCAG_C
Cuts at: 0 331 491 671 863 896 1326
Size: 331 160 180 192 33 430
Fragments arranged by size:
430 331 192 180 160 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1326
Size: 865 461
BsiEI CG_ry'CG
Cuts at: 0 111 1326
Size: 111 1215
BsiHKAI G_wGCw'C
Cuts at: 0 1000 1103 1326
Size: 1000 103 223
Fragments arranged by size:
1000 223 103
BsiWI C'GTAC_G
Cuts at: 0 413 1326
Size: 413 913
BsmBI CGTCTCn'nnnn_
Cuts at: 0 586 1326
Size: 586 740
Bsp1286I G_dGCh'C
Cuts at: 0 39 67 122 377 678 1000 1103 1326
Size: 39 28 55 255 301 322 103 223
Fragments arranged by size:
322 301 255 223 103 55 39 28
BspEI T'CCGG_A
Cuts at: 0 1127 1326
Size: 1127 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 458 1326
Size: 311 147 868
Fragments arranged by size:
868 311 147
BssHII G'CGCG_C
Cuts at: 0 603 1326
Size: 603 723
BssSI C'ACGA_G
Cuts at: 0 850 1326
Size: 850 476
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 628 1326
Size: 162 466 698
Fragments arranged by size:
698 466 162
BstYI r'GATC_y
Cuts at: 0 609 1172 1326
Size: 609 563 154
Fragments arranged by size:
609 563 154
Bsu36I CC'TnA_GG
Cuts at: 0 295 664 1326
Size: 295 369 662
Fragments arranged by size:
662 369 295
BtgI C'CryG_G
Cuts at: 0 111 134 202 211 393 464 476 1326
Size: 111 23 68 9 182 71 12 850
Fragments arranged by size:
850 182 111 71 68 23 12 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 114 1326
Size: 114 1212
BtsI GCAGTG_nn'
Cuts at: 0 1208 1326
Size: 1208 118
EaeI y'GGCC_r
Cuts at: 0 108 1326
Size: 108 1218
EagI C'GGCC_G
Cuts at: 0 108 1326
Size: 108 1218
EarI CTCTTCn'nnn_
Cuts at: 0 988 1108 1326
Size: 988 120 218
Fragments arranged by size:
988 218 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 484 1326
Size: 298 186 842
Fragments arranged by size:
842 298 186
EcoICRI GAG'CTC
Cuts at: 0 998 1326
Size: 998 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 562 577 836 980 1326
Size: 331 231 15 259 144 346
Fragments arranged by size:
346 331 259 231 144 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 1326
Size: 442 884
EcoO109I rG'GnC_Cy
Cuts at: 0 35 36 373 447 533 675 1326
Size: 35 1 337 74 86 142 651
Fragments arranged by size:
651 337 142 86 74 35 1
HaeII r_GCGC'y
Cuts at: 0 7 498 1326
Size: 7 491 828
Fragments arranged by size:
828 491 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 374 406 418 439 450 471
Size: 319 32 23 32 12 21 11 21
Cuts at: 471 938 953 970 985 1326
Size: 467 15 17 15 341
Fragments arranged by size:
467 341 319 32 32 23 21 21
17 15 15 12 11
HindIII A'AGCT_T
Cuts at: 0 1080 1326
Size: 1080 246
KasI G'GCGC_C
Cuts at: 0 3 1326
Size: 3 1323
MluI A'CGCG_T
Cuts at: 0 410 1326
Size: 410 916
MslI CAynn'nnrTG
Cuts at: 0 94 133 1326
Size: 94 39 1193
Fragments arranged by size:
1193 94 39
MspA1I CmG'CkG
Cuts at: 0 94 113 204 395 466 717 799 1052
Size: 94 19 91 191 71 251 82 253
Cuts at: 1052 1326
Size: 274
Fragments arranged by size:
274 253 251 191 94 91 82 71
19
NarI GG'CG_CC
Cuts at: 0 4 1326
Size: 4 1322
NspI r_CATG'y
Cuts at: 0 839 1170 1326
Size: 839 331 156
Fragments arranged by size:
839 331 156
PasI CC'CwG_GG
Cuts at: 0 678 1326
Size: 678 648
PciI A'CATG_T
Cuts at: 0 1166 1326
Size: 1166 160
PfoI T'CCnGG_A
Cuts at: 0 457 1326
Size: 457 869
PpuMI rG'GwC_Cy
Cuts at: 0 447 533 1326
Size: 447 86 793
Fragments arranged by size:
793 447 86
PspOMI G'GGCC_C
Cuts at: 0 35 373 674 1326
Size: 35 338 301 652
Fragments arranged by size:
652 338 301 35
PstI C_TGCA'G
Cuts at: 0 771 1035 1326
Size: 771 264 291
Fragments arranged by size:
771 291 264
SacI G_AGCT'C
Cuts at: 0 1000 1326
Size: 1000 326
SacII CC_GC'GG
Cuts at: 0 114 205 396 467 1326
Size: 114 91 191 71 859
Fragments arranged by size:
859 191 114 91 71
SapI GCTCTTCn'nnn_
Cuts at: 0 988 1108 1326
Size: 988 120 218
Fragments arranged by size:
988 218 120
SbfI CC_TGCA'GG
Cuts at: 0 771 1326
Size: 771 555
SfcI C'TryA_G
Cuts at: 0 598 767 1031 1326
Size: 598 169 264 295
Fragments arranged by size:
598 295 264 169
SfoI GGC'GCC
Cuts at: 0 5 1326
Size: 5 1321
SmlI C'TyrA_G
Cuts at: 0 75 1326
Size: 75 1251
SnaBI TAC'GTA
Cuts at: 0 144 1326
Size: 144 1182
StuI AGG'CCT
Cuts at: 0 765 1326
Size: 765 561
StyI C'CwwG_G
Cuts at: 0 263 942 1107 1326
Size: 263 679 165 219
Fragments arranged by size:
679 263 219 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 204 299 505 1150 1326
Size: 64 140 95 206 645 176
Fragments arranged by size:
645 206 176 140 95 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 1122 1146 1154 1178 1326
Size: 535 32 555 24 8 24 148
Fragments arranged by size:
555 535 148 32 24 24 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 328 355 912 1326
Size: 328 27 557 414
Fragments arranged by size:
557 414 328 27
Enzymes that do cut:
AccI AfeI AflIII AleI ApaI BanI BanII BbeI
BcgI BglI Bme1580I BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspMI BssHII BssSI BstAPI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
KasI MluI MslI MspA1I NarI NspI PasI PciI
PfoI PpuMI PspOMI PstI SacI SacII SapI SbfI
SfcI SfoI SmlI SnaBI StuI StyI TaqII TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaBI
BsmI BspHI BsrBI BsrDI BsrFI BsrGI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI