Restriction map for
pFN21AE1904
(Linear) (Six-base) MAPSORT of: KIEE1904.seq Check: 8058 from: 1 to: 1302
KIEE1904.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AfeI AGC'GCT
Cuts at: 0 397 1302
Size: 397 905
AflIII A'CryG_T
Cuts at: 0 1259 1302
Size: 1259 43
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 1302
Size: 383 32 887
Fragments arranged by size:
887 383 32
ApaI G_GGCC'C
Cuts at: 0 212 1302
Size: 212 1090
ApoI r'AATT_y
Cuts at: 0 485 552 766 1139 1225 1302
Size: 485 67 214 373 86 77
Fragments arranged by size:
485 373 214 86 77 67
AvaI C'yCGr_G
Cuts at: 0 173 1302
Size: 173 1129
BanII G_rGCy'C
Cuts at: 0 212 782 1302
Size: 212 570 520
Fragments arranged by size:
570 520 212
BciVI GTATCCnnnnn_n'
Cuts at: 0 1270 1302
Size: 1270 32
BclI T'GATC_A
Cuts at: 0 318 1302
Size: 318 984
BglII A'GATC_T
Cuts at: 0 714 1302
Size: 714 588
Bme1580I G_kGCm'C
Cuts at: 0 212 1302
Size: 212 1090
BmrI ACTGGGnnnn_n'
Cuts at: 0 135 1302
Size: 135 1167
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 206 1302
Size: 206 1096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1302
Size: 272 1030
Bpu10I CC'TnA_GC
Cuts at: 0 191 1045 1302
Size: 191 854 257
Fragments arranged by size:
854 257 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1302
Size: 757 545
BsaBI GATnn'nnATC
Cuts at: 0 1281 1302
Size: 1281 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1213 1243 1302
Size: 1213 30 59
Fragments arranged by size:
1213 59 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 261 371 1302
Size: 108 153 110 931
Fragments arranged by size:
931 153 110 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1302
Size: 64 1238
BsmI GAATG_Cn'
Cuts at: 0 30 1302
Size: 30 1272
Bsp1286I G_dGCh'C
Cuts at: 0 212 782 1302
Size: 212 570 520
Fragments arranged by size:
570 520 212
BsrBI CCG'CTC
Cuts at: 0 190 1302
Size: 190 1112
BssSI C'ACGA_G
Cuts at: 0 491 1302
Size: 491 811
BstBI TT'CG_AA
Cuts at: 0 798 1302
Size: 798 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 626 1273 1302
Size: 626 647 29
Fragments arranged by size:
647 626 29
BstYI r'GATC_y
Cuts at: 0 53 714 752 1302
Size: 53 661 38 550
Fragments arranged by size:
661 550 53 38
BtgI C'CryG_G
Cuts at: 0 366 1302
Size: 366 936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 921 956 1302
Size: 921 35 346
Fragments arranged by size:
921 346 35
DraI TTT'AAA
Cuts at: 0 311 1302
Size: 311 991
DraIII CAC_nnn'GTG
Cuts at: 0 845 1302
Size: 845 457
EaeI y'GGCC_r
Cuts at: 0 628 815 929 1146 1302
Size: 628 187 114 217 156
Fragments arranged by size:
628 217 187 156 114
EarI CTCTTCn'nnn_
Cuts at: 0 880 1086 1164 1296 1302
Size: 880 206 78 132 6
Fragments arranged by size:
880 206 132 78 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1302
Size: 272 1030
EcoO109I rG'GnC_Cy
Cuts at: 0 201 209 738 1302
Size: 201 8 529 564
Fragments arranged by size:
564 529 201 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 346 1302
Size: 346 956
HaeII r_GCGC'y
Cuts at: 0 133 399 1302
Size: 133 266 903
Fragments arranged by size:
903 266 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1302
Size: 1111 32 159
Fragments arranged by size:
1111 159 32
HincII GTy'rAC
Cuts at: 0 669 1302
Size: 669 633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1256 1302
Size: 1256 46
MscI TGG'CCA
Cuts at: 0 630 817 931 1148 1302
Size: 630 187 114 217 154
Fragments arranged by size:
630 217 187 154 114
NcoI C'CATG_G
Cuts at: 0 366 1302
Size: 366 936
NdeI CA'TA_TG
Cuts at: 0 686 1302
Size: 686 616
NspI r_CATG'y
Cuts at: 0 261 1263 1302
Size: 261 1002 39
Fragments arranged by size:
1002 261 39
PasI CC'CwG_GG
Cuts at: 0 205 619 1302
Size: 205 414 683
Fragments arranged by size:
683 414 205
PciI A'CATG_T
Cuts at: 0 1259 1302
Size: 1259 43
PpuMI rG'GwC_Cy
Cuts at: 0 201 738 1302
Size: 201 537 564
Fragments arranged by size:
564 537 201
PsiI TTA'TAA
Cuts at: 0 472 1302
Size: 472 830
PspOMI G'GGCC_C
Cuts at: 0 208 1302
Size: 208 1094
SapI GCTCTTCn'nnn_
Cuts at: 0 1086 1302
Size: 1086 216
SfcI C'TryA_G
Cuts at: 0 731 1302
Size: 731 571
SmlI C'TyrA_G
Cuts at: 0 173 772 1302
Size: 173 599 530
Fragments arranged by size:
599 530 173
SphI G_CATG'C
Cuts at: 0 261 1302
Size: 261 1041
StuI AGG'CCT
Cuts at: 0 77 1302
Size: 77 1225
StyI C'CwwG_G
Cuts at: 0 330 366 1302
Size: 330 36 936
Fragments arranged by size:
936 330 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1020 1125 1302
Size: 1020 105 177
Fragments arranged by size:
1020 177 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1213 1245 1302
Size: 1213 32 57
Fragments arranged by size:
1213 57 32
XhoI C'TCGA_G
Cuts at: 0 173 1302
Size: 173 1129
Enzymes that do cut:
AfeI AflIII AloI ApaI ApoI AvaI BanII BciVI
BclI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaBI BsaXI BseRI BsgI BsmI Bsp1286I BsrBI BssSI
BstBI BstXI BstYI BtgI CspCI DraI DraIII EaeI
EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII MmeI
MscI NcoI NdeI NspI PasI PciI PpuMI PsiI
PspOMI SapI SfcI SmlI SphI StuI StyI TaqII
TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI BmgBI BmtI BsaI BsaAI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MslI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI
PmeI PmlI PpiI PshAI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XmaI
XmnI ZraI