Restriction map for
pFN21AE1903
(Linear) (Six-base) MAPSORT of: KIEE1903.seq Check: 7696 from: 1 to: 1299
KIEE1903.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1299
Size: 1234 65
AfeI AGC'GCT
Cuts at: 0 31 1299
Size: 31 1268
AflIII A'CryG_T
Cuts at: 0 1007 1013 1299
Size: 1007 6 286
Fragments arranged by size:
1007 286 6
AgeI A'CCGG_T
Cuts at: 0 755 1299
Size: 755 544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 234 1299
Size: 234 1065
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 1299
Size: 82 32 1185
Fragments arranged by size:
1185 82 32
ApoI r'AATT_y
Cuts at: 0 493 1299
Size: 493 806
AvaI C'yCGr_G
Cuts at: 0 42 1299
Size: 42 1257
BanII G_rGCy'C
Cuts at: 0 69 968 988 1077 1299
Size: 69 899 20 89 222
Fragments arranged by size:
899 222 89 69 20
BglI GCCn_nnn'nGGC
Cuts at: 0 20 1299
Size: 20 1279
BglII A'GATC_T
Cuts at: 0 956 1299
Size: 956 343
BlpI GC'TnA_GC
Cuts at: 0 844 967 1299
Size: 844 123 332
Fragments arranged by size:
844 332 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 1074 1299
Size: 1074 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 973 1299
Size: 46 927 326
Fragments arranged by size:
927 326 46
BsaAI yAC'GTr
Cuts at: 0 123 1299
Size: 123 1176
BsaWI w'CCGG_w
Cuts at: 0 755 836 914 1299
Size: 755 81 78 385
Fragments arranged by size:
755 385 81 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 689 719 1129 1159 1299
Size: 463 30 196 30 410 30 140
Fragments arranged by size:
463 410 196 140 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 1299
Size: 715 584
BsiHKAI G_wGCw'C
Cuts at: 0 128 968 988 1299
Size: 128 840 20 311
Fragments arranged by size:
840 311 128 20
Bsp1286I G_dGCh'C
Cuts at: 0 69 128 968 988 1077 1299
Size: 69 59 840 20 89 222
Fragments arranged by size:
840 222 89 69 59 20
BspEI T'CCGG_A
Cuts at: 0 836 914 1299
Size: 836 78 385
Fragments arranged by size:
836 385 78
BsrBI CCG'CTC
Cuts at: 0 141 1299
Size: 141 1158
BsrDI GCAATG_nn'
Cuts at: 0 228 1223 1299
Size: 228 995 76
Fragments arranged by size:
995 228 76
BsrFI r'CCGG_y
Cuts at: 0 755 1299
Size: 755 544
BsrGI T'GTAC_A
Cuts at: 0 678 1010 1052 1299
Size: 678 332 42 247
Fragments arranged by size:
678 332 247 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 152 1299
Size: 152 1147
BstYI r'GATC_y
Cuts at: 0 956 1299
Size: 956 343
BtgI C'CryG_G
Cuts at: 0 275 1037 1299
Size: 275 762 262
Fragments arranged by size:
762 275 262
BtsI GCAGTG_nn'
Cuts at: 0 150 1299
Size: 150 1149
EaeI y'GGCC_r
Cuts at: 0 338 1299
Size: 338 961
EarI CTCTTCn'nnn_
Cuts at: 0 902 1299
Size: 902 397
EcoICRI GAG'CTC
Cuts at: 0 966 986 1299
Size: 966 20 313
Fragments arranged by size:
966 313 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 973 1234 1299
Size: 46 927 261 65
Fragments arranged by size:
927 261 65 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1216 1299
Size: 1216 83
EcoO109I rG'GnC_Cy
Cuts at: 0 12 656 1299
Size: 12 644 643
Fragments arranged by size:
644 643 12
EcoRI G'AATT_C
Cuts at: 0 493 1299
Size: 493 806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 785 1299
Size: 785 514
HaeII r_GCGC'y
Cuts at: 0 33 1299
Size: 33 1266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 866 898 1079 1111 1299
Size: 388 32 446 32 181 32 188
Fragments arranged by size:
446 388 188 181 32 32 32
HincII GTy'rAC
Cuts at: 0 135 537 1299
Size: 135 402 762
Fragments arranged by size:
762 402 135
HpaI GTT'AAC
Cuts at: 0 537 1299
Size: 537 762
MfeI C'AATT_G
Cuts at: 0 554 1299
Size: 554 745
MscI TGG'CCA
Cuts at: 0 340 1299
Size: 340 959
NcoI C'CATG_G
Cuts at: 0 275 1037 1299
Size: 275 762 262
Fragments arranged by size:
762 275 262
NspI r_CATG'y
Cuts at: 0 1017 1059 1299
Size: 1017 42 240
Fragments arranged by size:
1017 240 42
PciI A'CATG_T
Cuts at: 0 1013 1299
Size: 1013 286
PflMI CCAn_nnn'nTGG
Cuts at: 0 1038 1299
Size: 1038 261
PpuMI rG'GwC_Cy
Cuts at: 0 656 1299
Size: 656 643
PstI C_TGCA'G
Cuts at: 0 40 74 1299
Size: 40 34 1225
Fragments arranged by size:
1225 40 34
SacI G_AGCT'C
Cuts at: 0 968 988 1299
Size: 968 20 311
Fragments arranged by size:
968 311 20
SanDI GG'GwC_CC
Cuts at: 0 656 1299
Size: 656 643
ScaI AGT'ACT
Cuts at: 0 160 521 1299
Size: 160 361 778
Fragments arranged by size:
778 361 160
SfcI C'TryA_G
Cuts at: 0 36 70 1107 1299
Size: 36 34 1037 192
Fragments arranged by size:
1037 192 36 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 20 1299
Size: 20 1279
SmaI CCC'GGG
Cuts at: 0 44 1299
Size: 44 1255
SrfI GCCC'GGGC
Cuts at: 0 44 1299
Size: 44 1255
StyI C'CwwG_G
Cuts at: 0 275 806 824 1037 1299
Size: 275 531 18 213 262
Fragments arranged by size:
531 275 262 213 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 371 1030 1299
Size: 371 659 269
Fragments arranged by size:
659 371 269
TatI w'GTAC_w
Cuts at: 0 158 519 678 1010 1052 1127 1299
Size: 158 361 159 332 42 75 172
Fragments arranged by size:
361 332 172 159 158 75 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 919 951 1299
Size: 463 32 424 32 348
Fragments arranged by size:
463 424 348 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 880 1299
Size: 880 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 876 1230 1299
Size: 258 618 354 69
Fragments arranged by size:
618 354 258 69
XmaI C'CCGG_G
Cuts at: 0 42 1299
Size: 42 1257
XmnI GAAnn'nnTTC
Cuts at: 0 369 1299
Size: 369 930
Enzymes that do cut:
AcuI AfeI AflIII AgeI AlfI AloI ApoI AvaI
BanII BglI BglII BlpI BmrI BpmI BsaAI BsaWI
BsaXI BseRI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrFI
BsrGI BstAPI BstYI BtgI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HpaI MfeI MscI NcoI NspI PciI PflMI PpuMI
PstI SacI SanDI ScaI SfcI SfiI SmaI SrfI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AleI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BspMI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRV FseI FspI FspAI HindIII KasI KpnI
MluI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI XbaI XhoI ZraI