Restriction map for
pFN21AE1898
(Linear) (Six-base) MAPSORT of: KIEE1898.seq Check: 8025 from: 1 to: 2472
KIEE1898.orf
With 182 enzymes: SgfI *
August 13, 2015 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1507 2472
Size: 1507 965
AccI GT'mk_AC
Cuts at: 0 599 2472
Size: 599 1873
AclI AA'CG_TT
Cuts at: 0 264 2472
Size: 264 2208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 635 1703 2472
Size: 527 108 1068 769
Fragments arranged by size:
1068 769 527 108
AflIII A'CryG_T
Cuts at: 0 192 351 668 670 2472
Size: 192 159 317 2 1802
Fragments arranged by size:
1802 317 192 159 2
AlwNI CAG_nnn'CTG
Cuts at: 0 1514 1761 2472
Size: 1514 247 711
Fragments arranged by size:
1514 711 247
ApoI r'AATT_y
Cuts at: 0 276 2472
Size: 276 2196
AseI AT'TA_AT
Cuts at: 0 1613 2472
Size: 1613 859
AvaI C'yCGr_G
Cuts at: 0 69 94 1440 2472
Size: 69 25 1346 1032
Fragments arranged by size:
1346 1032 69 25
BanI G'GyrC_C
Cuts at: 0 48 2241 2472
Size: 48 2193 231
Fragments arranged by size:
2193 231 48
BbeI G_GCGC'C
Cuts at: 0 52 2245 2472
Size: 52 2193 227
Fragments arranged by size:
2193 227 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 2297 2472
Size: 2297 175
BbvCI CC'TCA_GC
Cuts at: 0 977 2472
Size: 977 1495
BciVI GTATCCnnnnn_n'
Cuts at: 0 1316 2472
Size: 1316 1156
BfrBI ATG'CAT
Cuts at: 0 1733 2472
Size: 1733 739
BglII A'GATC_T
Cuts at: 0 174 2472
Size: 174 2298
BlpI GC'TnA_GC
Cuts at: 0 1975 2146 2472
Size: 1975 171 326
Fragments arranged by size:
1975 326 171
Bme1580I G_kGCm'C
Cuts at: 0 1272 1666 1838 2472
Size: 1272 394 172 634
Fragments arranged by size:
1272 634 394 172
BmrI ACTGGGnnnn_n'
Cuts at: 0 1108 2123 2472
Size: 1108 1015 349
Fragments arranged by size:
1108 1015 349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 387 2472
Size: 26 361 2085
Fragments arranged by size:
2085 361 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1655 1783 2189 2472
Size: 1655 128 406 283
Fragments arranged by size:
1655 406 283 128
Bpu10I CC'TnA_GC
Cuts at: 0 977 2472
Size: 977 1495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 2472
Size: 838 1634
BsaAI yAC'GTr
Cuts at: 0 354 671 2472
Size: 354 317 1801
Fragments arranged by size:
1801 354 317
BsaHI Gr'CG_yC
Cuts at: 0 49 2242 2472
Size: 49 2193 230
Fragments arranged by size:
2193 230 49
BsaWI w'CCGG_w
Cuts at: 0 60 1248 2394 2472
Size: 60 1188 1146 78
Fragments arranged by size:
1188 1146 78 60
BseYI C'CCAG_C
Cuts at: 0 2296 2472
Size: 2296 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1500 1560 1626 2472
Size: 1500 60 66 846
Fragments arranged by size:
1500 846 66 60
BsiHKAI G_wGCw'C
Cuts at: 0 1159 2472
Size: 1159 1313
BsmI GAATG_Cn'
Cuts at: 0 156 848 2063 2472
Size: 156 692 1215 409
Fragments arranged by size:
1215 692 409 156
Bsp1286I G_dGCh'C
Cuts at: 0 1159 1272 1666 1838 2472
Size: 1159 113 394 172 634
Fragments arranged by size:
1159 634 394 172 113
BspEI T'CCGG_A
Cuts at: 0 60 1248 2472
Size: 60 1188 1224
Fragments arranged by size:
1224 1188 60
BspHI T'CATG_A
Cuts at: 0 1212 2472
Size: 1212 1260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 799 1507 2472
Size: 799 708 965
Fragments arranged by size:
965 799 708
BsrGI T'GTAC_A
Cuts at: 0 1888 2472
Size: 1888 584
BssSI C'ACGA_G
Cuts at: 0 1330 2472
Size: 1330 1142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1267 1661 1805 2472
Size: 1267 394 144 667
Fragments arranged by size:
1267 667 394 144
BstEII G'GTnAC_C
Cuts at: 0 2397 2472
Size: 2397 75
BstYI r'GATC_y
Cuts at: 0 174 654 1704 2379 2472
Size: 174 480 1050 675 93
Fragments arranged by size:
1050 675 480 174 93
BtgI C'CryG_G
Cuts at: 0 1080 2472
Size: 1080 1392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 682 2472
Size: 682 1790
BtsI GCAGTG_nn'
Cuts at: 0 1548 1662 2472
Size: 1548 114 810
Fragments arranged by size:
1548 810 114
DraI TTT'AAA
Cuts at: 0 237 560 816 2427 2472
Size: 237 323 256 1611 45
Fragments arranged by size:
1611 323 256 237 45
DrdI GACnn_nn'nnGTC
Cuts at: 0 2395 2472
Size: 2395 77
EaeI y'GGCC_r
Cuts at: 0 1824 2472
Size: 1824 648
EarI CTCTTCn'nnn_
Cuts at: 0 569 611 636 722 1454 2472
Size: 569 42 25 86 732 1018
Fragments arranged by size:
1018 732 569 86 42 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 635 1655 1703 1783 2189 2472
Size: 527 108 1020 48 80 406 283
Fragments arranged by size:
1020 527 406 283 108 80 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 2472
Size: 1186 1286
EcoO109I rG'GnC_Cy
Cuts at: 0 1815 2337 2472
Size: 1815 522 135
Fragments arranged by size:
1815 522 135
EcoRV GAT'ATC
Cuts at: 0 513 2472
Size: 513 1959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2358 2472
Size: 2358 114
HaeII r_GCGC'y
Cuts at: 0 52 2245 2472
Size: 52 2193 227
Fragments arranged by size:
2193 227 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 970 1002 1311 1343 2149 2181
Size: 118 32 820 32 309 32 806 32
Cuts at: 2181 2472
Size: 291
Fragments arranged by size:
820 806 309 291 118 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2451 2472
Size: 2451 21
HindIII A'AGCT_T
Cuts at: 0 100 2472
Size: 100 2372
KasI G'GCGC_C
Cuts at: 0 48 2241 2472
Size: 48 2193 231
Fragments arranged by size:
2193 231 48
MslI CAynn'nnrTG
Cuts at: 0 1085 1315 2472
Size: 1085 230 1157
Fragments arranged by size:
1157 1085 230
MspA1I CmG'CkG
Cuts at: 0 1761 1829 1974 2362 2472
Size: 1761 68 145 388 110
Fragments arranged by size:
1761 388 145 110 68
NarI GG'CG_CC
Cuts at: 0 49 2242 2472
Size: 49 2193 230
Fragments arranged by size:
2193 230 49
NcoI C'CATG_G
Cuts at: 0 1080 2472
Size: 1080 1392
NdeI CA'TA_TG
Cuts at: 0 2312 2472
Size: 2312 160
NsiI A_TGCA'T
Cuts at: 0 1735 2472
Size: 1735 737
NspI r_CATG'y
Cuts at: 0 196 1778 2472
Size: 196 1582 694
Fragments arranged by size:
1582 694 196
PciI A'CATG_T
Cuts at: 0 192 2472
Size: 192 2280
PflMI CCAn_nnn'nTGG
Cuts at: 0 2079 2274 2472
Size: 2079 195 198
Fragments arranged by size:
2079 198 195
PfoI T'CCnGG_A
Cuts at: 0 1671 2285 2472
Size: 1671 614 187
Fragments arranged by size:
1671 614 187
PmlI CAC'GTG
Cuts at: 0 354 671 2472
Size: 354 317 1801
Fragments arranged by size:
1801 354 317
PpuMI rG'GwC_Cy
Cuts at: 0 1815 2337 2472
Size: 1815 522 135
Fragments arranged by size:
1815 522 135
PsiI TTA'TAA
Cuts at: 0 409 500 2472
Size: 409 91 1972
Fragments arranged by size:
1972 409 91
PspXI vC'TCGA_Gb
Cuts at: 0 1440 2472
Size: 1440 1032
PstI C_TGCA'G
Cuts at: 0 1360 1813 2219 2435 2472
Size: 1360 453 406 216 37
Fragments arranged by size:
1360 453 406 216 37
PvuII CAG'CTG
Cuts at: 0 1761 1974 2362 2472
Size: 1761 213 388 110
Fragments arranged by size:
1761 388 213 110
SapI GCTCTTCn'nnn_
Cuts at: 0 611 636 2472
Size: 611 25 1836
Fragments arranged by size:
1836 611 25
ScaI AGT'ACT
Cuts at: 0 258 2472
Size: 258 2214
SexAI A'CCwGG_T
Cuts at: 0 876 1199 2472
Size: 876 323 1273
Fragments arranged by size:
1273 876 323
SfcI C'TryA_G
Cuts at: 0 178 705 1356 1809 2215 2431 2472
Size: 178 527 651 453 406 216 41
Fragments arranged by size:
651 527 453 406 216 178 41
SfoI GGC'GCC
Cuts at: 0 50 2243 2472
Size: 50 2193 229
Fragments arranged by size:
2193 229 50
SmaI CCC'GGG
Cuts at: 0 71 2472
Size: 71 2401
SmlI C'TyrA_G
Cuts at: 0 94 853 1440 2472
Size: 94 759 587 1032
Fragments arranged by size:
1032 759 587 94
StyI C'CwwG_G
Cuts at: 0 1080 2472
Size: 1080 1392
TatI w'GTAC_w
Cuts at: 0 256 317 733 1888 1932 2472
Size: 256 61 416 1155 44 540
Fragments arranged by size:
1155 540 416 256 61 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2376 2408 2472
Size: 2376 32 64
Fragments arranged by size:
2376 64 32
XhoI C'TCGA_G
Cuts at: 0 94 1440 2472
Size: 94 1346 1032
Fragments arranged by size:
1346 1032 94
XmaI C'CCGG_G
Cuts at: 0 69 2472
Size: 69 2403
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AlwNI ApoI AseI
AvaI BanI BbeI BbsI BbvCI BciVI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrGI BssSI BstAPI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI
MslI MspA1I NarI NcoI NdeI NsiI NspI PciI
PflMI PfoI PmlI PpuMI PsiI PspXI PstI PvuII
SapI ScaI SexAI SfcI SfoI SmaI SmlI StyI
TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanII BcgI BclI BglI BmgBI BmtI BsaI BsaBI
BsaXI BseRI BsiEI BsiWI BsmBI BsrBI BsrDI BsrFI
BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EagI EciI EcoICRI EcoRI FseI FspI FspAI HpaI
KpnI MfeI MluI MmeI MscI NaeI NgoMIV NheI
NotI NruI PacI PasI PmeI PpiI PshAI PspOMI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XmnI ZraI