Restriction map for
pFN21AE1895
(Linear) (Six-base) MAPSORT of: KIEE1895.seq Check: 8243 from: 1 to: 1251
KIEE1895.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 214 873 1251
Size: 214 659 378
Fragments arranged by size:
659 378 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1251
Size: 190 1061
AfeI AGC'GCT
Cuts at: 0 568 1141 1251
Size: 568 573 110
Fragments arranged by size:
573 568 110
AlwNI CAG_nnn'CTG
Cuts at: 0 149 1251
Size: 149 1102
ApaI G_GGCC'C
Cuts at: 0 66 72 438 997 1251
Size: 66 6 366 559 254
Fragments arranged by size:
559 366 254 66 6
AvaI C'yCGr_G
Cuts at: 0 65 856 1251
Size: 65 791 395
Fragments arranged by size:
791 395 65
BanI G'GyrC_C
Cuts at: 0 1145 1251
Size: 1145 106
BanII G_rGCy'C
Cuts at: 0 66 72 191 438 921 997 1251
Size: 66 6 119 247 483 76 254
Fragments arranged by size:
483 254 247 119 76 66 6
BbeI G_GCGC'C
Cuts at: 0 1149 1251
Size: 1149 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1226 1251
Size: 1226 25
BglI GCCn_nnn'nGGC
Cuts at: 0 500 1251
Size: 500 751
BglII A'GATC_T
Cuts at: 0 809 1251
Size: 809 442
Bme1580I G_kGCm'C
Cuts at: 0 66 72 164 270 438 997 1251
Size: 66 6 92 106 168 559 254
Fragments arranged by size:
559 254 168 106 92 66 6
BmgBI CAC'GTC
Cuts at: 0 1011 1251
Size: 1011 240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 1251
Size: 312 939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 609 1210 1251
Size: 331 278 601 41
Fragments arranged by size:
601 331 278 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1251
Size: 1120 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 853 1109 1251
Size: 853 256 142
Fragments arranged by size:
853 256 142
BsaHI Gr'CG_yC
Cuts at: 0 1146 1251
Size: 1146 105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 1251
Size: 34 1217
BseYI C'CCAG_C
Cuts at: 0 57 153 715 910 960 1026 1059 1251
Size: 57 96 562 195 50 66 33 192
Fragments arranged by size:
562 195 192 96 66 57 50 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 798 1251
Size: 424 374 453
Fragments arranged by size:
453 424 374
BsiEI CG_ry'CG
Cuts at: 0 13 1107 1251
Size: 13 1094 144
Fragments arranged by size:
1094 144 13
BsiHKAI G_wGCw'C
Cuts at: 0 547 617 921 1251
Size: 547 70 304 330
Fragments arranged by size:
547 330 304 70
Bsp1286I G_dGCh'C
Cuts at: 0 66 72 164 191 270 438 547 617
Size: 66 6 92 27 79 168 109 70
Cuts at: 617 921 997 1251
Size: 304 76 254
Fragments arranged by size:
304 254 168 109 92 79 76 70
66 27 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 214 499 873 1251
Size: 214 285 374 378
Fragments arranged by size:
378 374 285 214
BsrFI r'CCGG_y
Cuts at: 0 106 741 964 1251
Size: 106 635 223 287
Fragments arranged by size:
635 287 223 106
BssSI C'ACGA_G
Cuts at: 0 540 1251
Size: 540 711
BstAPI GCAn_nnn'nTGC
Cuts at: 0 149 1251
Size: 149 1102
BstYI r'GATC_y
Cuts at: 0 809 1251
Size: 809 442
BtgI C'CryG_G
Cuts at: 0 13 1088 1160 1251
Size: 13 1075 72 91
Fragments arranged by size:
1075 91 72 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 533 1251
Size: 533 718
BtsI GCAGTG_nn'
Cuts at: 0 332 675 807 1251
Size: 332 343 132 444
Fragments arranged by size:
444 343 332 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 192 227 1251
Size: 192 35 1024
Fragments arranged by size:
1024 192 35
EaeI y'GGCC_r
Cuts at: 0 10 329 743 1104 1251
Size: 10 319 414 361 147
Fragments arranged by size:
414 361 319 147 10
EagI C'GGCC_G
Cuts at: 0 10 1104 1251
Size: 10 1094 147
Fragments arranged by size:
1094 147 10
EcoICRI GAG'CTC
Cuts at: 0 919 1251
Size: 919 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 331 609 1210 1251
Size: 190 141 278 601 41
Fragments arranged by size:
601 278 190 141 41
EcoO109I rG'GnC_Cy
Cuts at: 0 62 174 434 435 488 1251
Size: 62 112 260 1 53 763
Fragments arranged by size:
763 260 112 62 53 1
FspI TGC'GCA
Cuts at: 0 1054 1228 1251
Size: 1054 174 23
Fragments arranged by size:
1054 174 23
FspAI rTGC'GCAy
Cuts at: 0 1054 1251
Size: 1054 197
HaeII r_GCGC'y
Cuts at: 0 43 310 570 773 874 1038 1143 1149
Size: 43 267 260 203 101 164 105 6
Cuts at: 1149 1251
Size: 102
Fragments arranged by size:
267 260 203 164 105 102 101 43
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 711 743 1251
Size: 711 32 508
Fragments arranged by size:
711 508 32
KasI G'GCGC_C
Cuts at: 0 1145 1251
Size: 1145 106
MslI CAynn'nnrTG
Cuts at: 0 518 1251
Size: 518 733
MspA1I CmG'CkG
Cuts at: 0 9 15 157 272 341 577 839 931
Size: 9 6 142 115 69 236 262 92
Cuts at: 931 1162 1251
Size: 231 89
Fragments arranged by size:
262 236 231 142 115 92 89 69
9 6
NaeI GCC'GGC
Cuts at: 0 966 1251
Size: 966 285
NarI GG'CG_CC
Cuts at: 0 1146 1251
Size: 1146 105
NgoMIV G'CCGG_C
Cuts at: 0 964 1251
Size: 964 287
NotI GC'GGCC_GC
Cuts at: 0 10 1251
Size: 10 1241
PasI CC'CwG_GG
Cuts at: 0 72 549 1251
Size: 72 477 702
Fragments arranged by size:
702 477 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1251
Size: 828 423
PpuMI rG'GwC_Cy
Cuts at: 0 488 1251
Size: 488 763
PspOMI G'GGCC_C
Cuts at: 0 62 68 434 993 1251
Size: 62 6 366 559 258
Fragments arranged by size:
559 366 258 62 6
PstI C_TGCA'G
Cuts at: 0 708 989 1241 1251
Size: 708 281 252 10
Fragments arranged by size:
708 281 252 10
PvuII CAG'CTG
Cuts at: 0 839 1251
Size: 839 412
SacI G_AGCT'C
Cuts at: 0 921 1251
Size: 921 330
SacII CC_GC'GG
Cuts at: 0 16 1163 1251
Size: 16 1147 88
Fragments arranged by size:
1147 88 16
SfcI C'TryA_G
Cuts at: 0 704 985 1237 1251
Size: 704 281 252 14
Fragments arranged by size:
704 281 252 14
SfoI GGC'GCC
Cuts at: 0 1147 1251
Size: 1147 104
SmaI CCC'GGG
Cuts at: 0 67 1251
Size: 67 1184
SmlI C'TyrA_G
Cuts at: 0 1135 1251
Size: 1135 116
SrfI GCCC'GGGC
Cuts at: 0 67 1251
Size: 67 1184
StuI AGG'CCT
Cuts at: 0 280 295 1251
Size: 280 15 956
Fragments arranged by size:
956 280 15
StyI C'CwwG_G
Cuts at: 0 761 1251
Size: 761 490
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 1251
Size: 763 488
Tth111I GACn'n_nGTC
Cuts at: 0 891 1021 1251
Size: 891 130 230
Fragments arranged by size:
891 230 130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 124 546 1034 1251
Size: 124 422 488 217
Fragments arranged by size:
488 422 217 124
XmaI C'CCGG_G
Cuts at: 0 65 1251
Size: 65 1186
XmnI GAAnn'nnTTC
Cuts at: 0 1045 1251
Size: 1045 206
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BmgBI BplI BpmI
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstAPI BstYI BtgI BtgZI
BtsI CspCI EaeI EagI EcoICRI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I KasI MslI MspA1I NaeI NarI
NgoMIV NotI PasI PflMI PpuMI PspOMI PstI PvuII
SacI SacII SfcI SfoI SmaI SmlI SrfI StuI
StyI TaqII Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI
DraIII DrdI EarI EciI EcoNI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI TatI TstI XbaI XhoI ZraI