Restriction map for
pFN21AE1894
(Linear) (Six-base) MAPSORT of: KIEE1894.seq Check: 8898 from: 1 to: 1269
KIEE1894.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 817 907 1269
Size: 217 600 90 362
Fragments arranged by size:
600 362 217 90
AflIII A'CryG_T
Cuts at: 0 129 1269
Size: 129 1140
AleI CACnn'nnGTG
Cuts at: 0 787 1269
Size: 787 482
ApaI G_GGCC'C
Cuts at: 0 273 1269
Size: 273 996
ApoI r'AATT_y
Cuts at: 0 341 1269
Size: 341 928
AvaI C'yCGr_G
Cuts at: 0 954 1269
Size: 954 315
BanI G'GyrC_C
Cuts at: 0 720 1269
Size: 720 549
BanII G_rGCy'C
Cuts at: 0 35 273 932 1269
Size: 35 238 659 337
Fragments arranged by size:
659 337 238 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 512 1269
Size: 478 34 757
Fragments arranged by size:
757 478 34
BclI T'GATC_A
Cuts at: 0 584 837 1202 1269
Size: 584 253 365 67
Fragments arranged by size:
584 365 253 67
BglII A'GATC_T
Cuts at: 0 579 1269
Size: 579 690
Bme1580I G_kGCm'C
Cuts at: 0 273 725 1108 1269
Size: 273 452 383 161
Fragments arranged by size:
452 383 273 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 920 1269
Size: 920 349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 970 1269
Size: 928 42 299
Fragments arranged by size:
928 299 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 717 1269
Size: 292 425 552
Fragments arranged by size:
552 425 292
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 1269
Size: 874 395
BsaAI yAC'GTr
Cuts at: 0 132 549 1269
Size: 132 417 720
Fragments arranged by size:
720 417 132
BsaBI GATnn'nnATC
Cuts at: 0 677 1269
Size: 677 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 784 814 1269
Size: 25 759 30 455
Fragments arranged by size:
759 455 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 784 1114 1269
Size: 106 678 330 155
Fragments arranged by size:
678 330 155 106
BseYI C'CCAG_C
Cuts at: 0 43 990 1269
Size: 43 947 279
Fragments arranged by size:
947 279 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1269
Size: 765 504
BsiHKAI G_wGCw'C
Cuts at: 0 932 1269
Size: 932 337
Bsp1286I G_dGCh'C
Cuts at: 0 35 273 725 932 1108 1269
Size: 35 238 452 207 176 161
Fragments arranged by size:
452 238 207 176 161 35
BspHI T'CATG_A
Cuts at: 0 1238 1269
Size: 1238 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 944 1269
Size: 944 325
BsrDI GCAATG_nn'
Cuts at: 0 816 1269
Size: 816 453
BsrGI T'GTAC_A
Cuts at: 0 179 1269
Size: 179 1090
BstAPI GCAn_nnn'nTGC
Cuts at: 0 366 1120 1269
Size: 366 754 149
Fragments arranged by size:
754 366 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 1269
Size: 249 1020
BstYI r'GATC_y
Cuts at: 0 579 1269
Size: 579 690
BtgI C'CryG_G
Cuts at: 0 785 1269
Size: 785 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 404 1269
Size: 404 865
DraI TTT'AAA
Cuts at: 0 639 1269
Size: 639 630
EarI CTCTTCn'nnn_
Cuts at: 0 829 1269
Size: 829 440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 141 1269
Size: 141 1128
EcoICRI GAG'CTC
Cuts at: 0 930 1269
Size: 930 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 817 907 928 970 1269
Size: 217 600 90 21 42 299
Fragments arranged by size:
600 299 217 90 42 21
EcoO109I rG'GnC_Cy
Cuts at: 0 201 269 591 1190 1269
Size: 201 68 322 599 79
Fragments arranged by size:
599 322 201 79 68
FspI TGC'GCA
Cuts at: 0 370 1269
Size: 370 899
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 723 755 760 792 1269
Size: 583 32 108 32 5 32 477
Fragments arranged by size:
583 477 108 32 32 32 5
MslI CAynn'nnrTG
Cuts at: 0 787 1269
Size: 787 482
MspA1I CmG'CkG
Cuts at: 0 402 520 990 1269
Size: 402 118 470 279
Fragments arranged by size:
470 402 279 118
PfoI T'CCnGG_A
Cuts at: 0 761 1269
Size: 761 508
PmlI CAC'GTG
Cuts at: 0 132 1269
Size: 132 1137
PpuMI rG'GwC_Cy
Cuts at: 0 591 1269
Size: 591 678
PspOMI G'GGCC_C
Cuts at: 0 269 1269
Size: 269 1000
PstI C_TGCA'G
Cuts at: 0 401 525 1269
Size: 401 124 744
Fragments arranged by size:
744 401 124
PvuII CAG'CTG
Cuts at: 0 402 520 990 1269
Size: 402 118 470 279
Fragments arranged by size:
470 402 279 118
SacI G_AGCT'C
Cuts at: 0 932 1269
Size: 932 337
SfcI C'TryA_G
Cuts at: 0 397 521 1214 1269
Size: 397 124 693 55
Fragments arranged by size:
693 397 124 55
SmlI C'TyrA_G
Cuts at: 0 307 696 954 1269
Size: 307 389 258 315
Fragments arranged by size:
389 315 307 258
StuI AGG'CCT
Cuts at: 0 305 493 1269
Size: 305 188 776
Fragments arranged by size:
776 305 188
StyI C'CwwG_G
Cuts at: 0 248 1269
Size: 248 1021
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 1237 1269
Size: 134 1103 32
Fragments arranged by size:
1103 134 32
TatI w'GTAC_w
Cuts at: 0 179 800 1269
Size: 179 621 469
Fragments arranged by size:
621 469 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 812 844 1269
Size: 812 32 425
Fragments arranged by size:
812 425 32
XhoI C'TCGA_G
Cuts at: 0 954 1269
Size: 954 315
XmnI GAAnn'nnTTC
Cuts at: 0 1231 1269
Size: 1231 38
Enzymes that do cut:
AcuI AflIII AleI ApaI ApoI AvaI BanI BanII
BcgI BclI BglII Bme1580I BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstXI BstYI BtgI
BtgZI DraI EarI EciI EcoICRI Eco57MI EcoO109I FspI
Hin4I MslI MspA1I PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SfcI SmlI StuI StyI TaqII TatI
TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI
BglI BlpI BmgBI BmrI BmtI Bpu10I BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraIII DrdI EaeI EagI EcoNI EcoRI EcoRV FalI
FseI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XmaI
ZraI