(Linear) (Six-base) MAPSORT of: KIEE1893.seq  Check: 3509  from: 1  to: 1266



KIEE1893.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:50  ..

Acc65I G'GTAC_C

Cuts at:      0     425    1266 
   Size:        425     841
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     772    1266 
   Size:        229     543     494

  Fragments arranged by size: 

                 543     494     229
 
AflIII A'CryG_T

Cuts at:      0     758    1266 
   Size:        758     508
 
AhdI GACnn_n'nnGTC

Cuts at:      0     636    1266 
   Size:        636     630
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     793     879    1266 
   Size:        793      86     387

  Fragments arranged by size: 

                 793     387      86
 
AlwNI CAG_nnn'CTG

Cuts at:      0     459    1002    1266 
   Size:        459     543     264

  Fragments arranged by size: 

                 543     459     264
 
ApoI r'AATT_y

Cuts at:      0     231    1117    1266 
   Size:        231     886     149

  Fragments arranged by size: 

                 886     231     149
 
AvaI C'yCGr_G

Cuts at:      0     557    1266 
   Size:        557     709
 
BanI G'GyrC_C

Cuts at:      0     425    1266 
   Size:        425     841
 
BanII G_rGCy'C

Cuts at:      0      36     938    1266 
   Size:         36     902     328

  Fragments arranged by size: 

                 902     328      36
 
BmgBI CAC'GTC

Cuts at:      0    1080    1266 
   Size:       1080     186
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      30     166    1168    1266 
   Size:         30     136    1002      98

  Fragments arranged by size: 

                1002     136      98      30
 
BmtI G_CTAG'C

Cuts at:      0     881    1266 
   Size:        881     385
 
Bpu10I CC'TnA_GC

Cuts at:      0     538     872    1266 
   Size:        538     334     394

  Fragments arranged by size: 

                 538     394     334
 
BsiEI CG_ry'CG

Cuts at:      0      57    1266 
   Size:         57    1209
 
BsiHKAI G_wGCw'C

Cuts at:      0     938    1266 
   Size:        938     328
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     938    1266 
   Size:         36     902     328

  Fragments arranged by size: 

                 902     328      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     554    1266 
   Size:        554     712
 
BsrBI CCG'CTC

Cuts at:      0     815    1266 
   Size:        815     451
 
BsrDI GCAATG_nn'

Cuts at:      0     436    1266 
   Size:        436     830
 
BstYI r'GATC_y

Cuts at:      0     160    1266 
   Size:        160    1106
 
BtsI GCAGTG_nn'

Cuts at:      0     131    1266 
   Size:        131    1135
 
EaeI y'GGCC_r

Cuts at:      0     968    1266 
   Size:        968     298
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     278     656    1266 
   Size:        113     165     378     610

  Fragments arranged by size: 

                 610     378     165     113
 
EcoICRI GAG'CTC

Cuts at:      0     936    1266 
   Size:        936     330
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     772    1266 
   Size:        229     543     494

  Fragments arranged by size: 

                 543     494     229
 
EcoRI G'AATT_C

Cuts at:      0     231    1266 
   Size:        231    1035
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     526    1266 
   Size:        526     740
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     622     654    1266 
   Size:        622      32     612

  Fragments arranged by size: 

                 622     612      32
 
HindIII A'AGCT_T

Cuts at:      0     380     820    1266 
   Size:        380     440     446

  Fragments arranged by size: 

                 446     440     380
 
KpnI G_GTAC'C

Cuts at:      0     429    1266 
   Size:        429     837
 
MscI TGG'CCA

Cuts at:      0     970    1266 
   Size:        970     296
 
MslI CAynn'nnrTG

Cuts at:      0     861    1266 
   Size:        861     405
 
MspA1I CmG'CkG

Cuts at:      0     456    1266 
   Size:        456     810
 
NheI G'CTAG_C

Cuts at:      0     877    1266 
   Size:        877     389
 
NspI r_CATG'y

Cuts at:      0     636     762    1266 
   Size:        636     126     504

  Fragments arranged by size: 

                 636     504     126
 
PciI A'CATG_T

Cuts at:      0     758    1266 
   Size:        758     508
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     384     807    1266 
   Size:        384     423     459

  Fragments arranged by size: 

                 459     423     384
 
PfoI T'CCnGG_A

Cuts at:      0     149     938    1266 
   Size:        149     789     328

  Fragments arranged by size: 

                 789     328     149
 
PshAI GACnn'nnGTC

Cuts at:      0     399    1266 
   Size:        399     867
 
PstI C_TGCA'G

Cuts at:      0     192    1266 
   Size:        192    1074
 
PvuII CAG'CTG

Cuts at:      0     456    1266 
   Size:        456     810
 
SacI G_AGCT'C

Cuts at:      0     938    1266 
   Size:        938     328
 
SfcI C'TryA_G

Cuts at:      0     188     305    1266 
   Size:        188     117     961

  Fragments arranged by size: 

                 961     188     117
 
StuI AGG'CCT

Cuts at:      0      64    1266 
   Size:         64    1202
 
StyI C'CwwG_G

Cuts at:      0     626     734    1070    1266 
   Size:        626     108     336     196

  Fragments arranged by size: 

                 626     336     196     108
 
TatI w'GTAC_w

Cuts at:      0     407     504     755    1032    1266 
   Size:        407      97     251     277     234

  Fragments arranged by size: 

                 407     277     251     234      97
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1077    1266 
   Size:       1077     189
 
XmnI GAAnn'nnTTC

Cuts at:      0    1208    1266 
   Size:       1208      58
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI     AlfI    AlwNI     ApoI     AvaI
     BanI    BanII    BmgBI     BmrI     BmtI   Bpu10I    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI    BstYI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI    EcoRI     FalI    Hin4I  HindIII     KpnI     MscI
     MslI   MspA1I     NheI     NspI     PciI    PflMI     PfoI    PshAI
     PstI    PvuII     SacI     SfcI     StuI     StyI     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI