Restriction map for
pFN21AE1888
(Linear) (Six-base) MAPSORT of: KIEE1888.seq Check: 6047 from: 1 to: 1248
KIEE1888.orf
With 182 enzymes: SgfI *
July 15, 2010 18:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 262 1248
Size: 262 986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1248
Size: 700 548
AhdI GACnn_n'nnGTC
Cuts at: 0 186 1248
Size: 186 1062
AlwNI CAG_nnn'CTG
Cuts at: 0 432 726 747 1248
Size: 432 294 21 501
Fragments arranged by size:
501 432 294 21
ApaI G_GGCC'C
Cuts at: 0 413 1248
Size: 413 835
AvaI C'yCGr_G
Cuts at: 0 135 235 1248
Size: 135 100 1013
Fragments arranged by size:
1013 135 100
BanI G'GyrC_C
Cuts at: 0 62 260 498 836 1248
Size: 62 198 238 338 412
Fragments arranged by size:
412 338 238 198 62
BanII G_rGCy'C
Cuts at: 0 98 105 413 1248
Size: 98 7 308 835
Fragments arranged by size:
835 308 98 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 185 202 1248
Size: 185 17 1046
Fragments arranged by size:
1046 185 17
BbvCI CC'TCA_GC
Cuts at: 0 245 1168 1248
Size: 245 923 80
Fragments arranged by size:
923 245 80
BclI T'GATC_A
Cuts at: 0 980 1248
Size: 980 268
BglII A'GATC_T
Cuts at: 0 155 1248
Size: 155 1093
BlpI GC'TnA_GC
Cuts at: 0 563 1101 1248
Size: 563 538 147
Fragments arranged by size:
563 538 147
Bme1580I G_kGCm'C
Cuts at: 0 67 265 413 501 1248
Size: 67 198 148 88 747
Fragments arranged by size:
747 198 148 88 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 804 1248
Size: 345 459 444
Fragments arranged by size:
459 444 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 421 442 499 589 778 1248
Size: 169 252 21 57 90 189 470
Fragments arranged by size:
470 252 189 169 90 57 21
Bpu10I CC'TnA_GC
Cuts at: 0 12 245 688 1096 1168 1248
Size: 12 233 443 408 72 80
Fragments arranged by size:
443 408 233 80 72 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1248
Size: 1207 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 1231 1248
Size: 1231 17
BsaAI yAC'GTr
Cuts at: 0 1200 1248
Size: 1200 48
BsaHI Gr'CG_yC
Cuts at: 0 1079 1248
Size: 1079 169
BsaWI w'CCGG_w
Cuts at: 0 1022 1248
Size: 1022 226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 796 1248
Size: 535 261 452
Fragments arranged by size:
535 452 261
BseYI C'CCAG_C
Cuts at: 0 387 879 1248
Size: 387 492 369
Fragments arranged by size:
492 387 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 1248
Size: 134 1114
BsiHKAI G_wGCw'C
Cuts at: 0 1070 1248
Size: 1070 178
Bsp1286I G_dGCh'C
Cuts at: 0 67 98 105 265 413 501 1070 1248
Size: 67 31 7 160 148 88 569 178
Fragments arranged by size:
569 178 160 148 88 67 31 7
BspEI T'CCGG_A
Cuts at: 0 1022 1248
Size: 1022 226
BspHI T'CATG_A
Cuts at: 0 365 542 983 1248
Size: 365 177 441 265
Fragments arranged by size:
441 365 265 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 262 359 449 1248
Size: 262 97 90 799
Fragments arranged by size:
799 262 97 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 768 1248
Size: 768 480
BstBI TT'CG_AA
Cuts at: 0 213 1248
Size: 213 1035
BstEII G'GTnAC_C
Cuts at: 0 324 1032 1248
Size: 324 708 216
Fragments arranged by size:
708 324 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 1248
Size: 420 828
BstYI r'GATC_y
Cuts at: 0 155 1248
Size: 155 1093
BtgI C'CryG_G
Cuts at: 0 413 458 1012 1248
Size: 413 45 554 236
Fragments arranged by size:
554 413 236 45
BtsI GCAGTG_nn'
Cuts at: 0 890 1248
Size: 890 358
DrdI GACnn_nn'nnGTC
Cuts at: 0 481 1248
Size: 481 767
EaeI y'GGCC_r
Cuts at: 0 644 1139 1248
Size: 644 495 109
Fragments arranged by size:
644 495 109
EarI CTCTTCn'nnn_
Cuts at: 0 877 976 1162 1248
Size: 877 99 186 86
Fragments arranged by size:
877 186 99 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 421 442 499 589 700 778 1248
Size: 169 252 21 57 90 111 78 470
Fragments arranged by size:
470 252 169 111 90 78 57 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 778 855 1113 1248
Size: 613 165 77 258 135
Fragments arranged by size:
613 258 165 135 77
EcoO109I rG'GnC_Cy
Cuts at: 0 84 474 744 1118 1248
Size: 84 390 270 374 130
Fragments arranged by size:
390 374 270 130 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1053 1248
Size: 1053 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 945 977 1084 1116 1139 1171
Size: 172 32 741 32 107 32 23 32
Cuts at: 1171 1248
Size: 77
Fragments arranged by size:
741 172 107 77 32 32 32 32
23
HindIII A'AGCT_T
Cuts at: 0 553 1248
Size: 553 695
MscI TGG'CCA
Cuts at: 0 646 1141 1248
Size: 646 495 107
Fragments arranged by size:
646 495 107
MslI CAynn'nnrTG
Cuts at: 0 1039 1248
Size: 1039 209
MspA1I CmG'CkG
Cuts at: 0 447 669 1248
Size: 447 222 579
Fragments arranged by size:
579 447 222
NcoI C'CATG_G
Cuts at: 0 413 1248
Size: 413 835
PasI CC'CwG_GG
Cuts at: 0 98 747 1248
Size: 98 649 501
Fragments arranged by size:
649 501 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 747 1248
Size: 747 501
PpuMI rG'GwC_Cy
Cuts at: 0 84 474 1248
Size: 84 390 774
Fragments arranged by size:
774 390 84
PspOMI G'GGCC_C
Cuts at: 0 409 1248
Size: 409 839
PstI C_TGCA'G
Cuts at: 0 446 452 536 1181 1248
Size: 446 6 84 645 67
Fragments arranged by size:
645 446 84 67 6
PvuII CAG'CTG
Cuts at: 0 447 669 1248
Size: 447 222 579
Fragments arranged by size:
579 447 222
SapI GCTCTTCn'nnn_
Cuts at: 0 976 1248
Size: 976 272
SfcI C'TryA_G
Cuts at: 0 442 448 532 1177 1248
Size: 442 6 84 645 71
Fragments arranged by size:
645 442 84 71 6
SmlI C'TyrA_G
Cuts at: 0 1222 1248
Size: 1222 26
StuI AGG'CCT
Cuts at: 0 628 787 1248
Size: 628 159 461
Fragments arranged by size:
628 461 159
StyI C'CwwG_G
Cuts at: 0 413 1248
Size: 413 835
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 50 248 1200 1248
Size: 50 198 952 48
Fragments arranged by size:
952 198 50 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 567 599 1145 1177 1248
Size: 567 32 546 32 71
Fragments arranged by size:
567 546 71 32 32
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BstAPI BstBI
BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MscI MslI
MspA1I NcoI PasI PflMI PpuMI PspOMI PstI PvuII
SapI SfcI SmlI StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI
BmgBI BmrI BmtI BsaBI BsaXI BsiEI BsiWI BsmI
BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI