Restriction map for
pFN21AE1886
(Linear) (Six-base) MAPSORT of: KIEE1886.seq Check: 6865 from: 1 to: 1236
KIEE1886.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1236
Size: 1130 106
AfeI AGC'GCT
Cuts at: 0 550 1236
Size: 550 686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 1236
Size: 586 650
AlwNI CAG_nnn'CTG
Cuts at: 0 207 339 981 1146 1236
Size: 207 132 642 165 90
Fragments arranged by size:
642 207 165 132 90
ApoI r'AATT_y
Cuts at: 0 319 448 745 1236
Size: 319 129 297 491
Fragments arranged by size:
491 319 297 129
AvaI C'yCGr_G
Cuts at: 0 227 870 1236
Size: 227 643 366
Fragments arranged by size:
643 366 227
BanI G'GyrC_C
Cuts at: 0 465 794 1236
Size: 465 329 442
Fragments arranged by size:
465 442 329
BanII G_rGCy'C
Cuts at: 0 24 509 1154 1236
Size: 24 485 645 82
Fragments arranged by size:
645 485 82 24
BbeI G_GCGC'C
Cuts at: 0 798 1236
Size: 798 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1103 1137 1236
Size: 1103 34 99
Fragments arranged by size:
1103 99 34
BfrBI ATG'CAT
Cuts at: 0 249 1236
Size: 249 987
BglII A'GATC_T
Cuts at: 0 137 786 1236
Size: 137 649 450
Fragments arranged by size:
649 450 137
Bme1580I G_kGCm'C
Cuts at: 0 470 586 1139 1236
Size: 470 116 553 97
Fragments arranged by size:
553 470 116 97
BmgBI CAC'GTC
Cuts at: 0 192 1236
Size: 192 1044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1218 1236
Size: 1218 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1168 1236
Size: 259 909 68
Fragments arranged by size:
909 259 68
Bpu10I CC'TnA_GC
Cuts at: 0 151 170 1032 1236
Size: 151 19 862 204
Fragments arranged by size:
862 204 151 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 784 1236
Size: 162 622 452
Fragments arranged by size:
622 452 162
BsaBI GATnn'nnATC
Cuts at: 0 739 1236
Size: 739 497
BsaHI Gr'CG_yC
Cuts at: 0 795 1236
Size: 795 441
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 949 1236
Size: 256 693 287
Fragments arranged by size:
693 287 256
BseYI C'CCAG_C
Cuts at: 0 475 1083 1236
Size: 475 608 153
Fragments arranged by size:
608 475 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1079 1236
Size: 255 824 157
Fragments arranged by size:
824 255 157
BsiHKAI G_wGCw'C
Cuts at: 0 350 509 536 1236
Size: 350 159 27 700
Fragments arranged by size:
700 350 159 27
BsmI GAATG_Cn'
Cuts at: 0 671 1236
Size: 671 565
Bsp1286I G_dGCh'C
Cuts at: 0 24 350 470 509 536 586 1139 1154
Size: 24 326 120 39 27 50 553 15
Cuts at: 1154 1236
Size: 82
Fragments arranged by size:
553 326 120 82 50 39 27 24
15
BspHI T'CATG_A
Cuts at: 0 839 1236
Size: 839 397
BsrDI GCAATG_nn'
Cuts at: 0 536 1236
Size: 536 700
BsrFI r'CCGG_y
Cuts at: 0 857 1236
Size: 857 379
BsrGI T'GTAC_A
Cuts at: 0 1091 1236
Size: 1091 145
BssSI C'ACGA_G
Cuts at: 0 727 1236
Size: 727 509
BstXI CCAn_nnnn'nTGG
Cuts at: 0 781 1212 1236
Size: 781 431 24
Fragments arranged by size:
781 431 24
BstYI r'GATC_y
Cuts at: 0 137 515 786 978 1236
Size: 137 378 271 192 258
Fragments arranged by size:
378 271 258 192 137
Bsu36I CC'TnA_GG
Cuts at: 0 1007 1236
Size: 1007 229
BtgI C'CryG_G
Cuts at: 0 53 774 780 1236
Size: 53 721 6 456
Fragments arranged by size:
721 456 53 6
BtsI GCAGTG_nn'
Cuts at: 0 584 1062 1236
Size: 584 478 174
Fragments arranged by size:
584 478 174
DraIII CAC_nnn'GTG
Cuts at: 0 732 1236
Size: 732 504
EaeI y'GGCC_r
Cuts at: 0 96 523 777 1236
Size: 96 427 254 459
Fragments arranged by size:
459 427 254 96
EarI CTCTTCn'nnn_
Cuts at: 0 106 514 636 1236
Size: 106 408 122 600
Fragments arranged by size:
600 408 122 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 358 1236
Size: 358 878
EcoICRI GAG'CTC
Cuts at: 0 507 1236
Size: 507 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1130 1168 1236
Size: 259 871 38 68
Fragments arranged by size:
871 259 68 38
EcoO109I rG'GnC_Cy
Cuts at: 0 204 1036 1143 1181 1236
Size: 204 832 107 38 55
Fragments arranged by size:
832 204 107 55 38
EcoRI G'AATT_C
Cuts at: 0 448 1236
Size: 448 788
EcoRV GAT'ATC
Cuts at: 0 711 1236
Size: 711 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 1236
Size: 27 1209
HaeII r_GCGC'y
Cuts at: 0 552 798 1236
Size: 552 246 438
Fragments arranged by size:
552 438 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 1236
Size: 583 32 621
Fragments arranged by size:
621 583 32
KasI G'GCGC_C
Cuts at: 0 794 1236
Size: 794 442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 674 996 1229 1236
Size: 272 402 322 233 7
Fragments arranged by size:
402 322 272 233 7
MslI CAynn'nnrTG
Cuts at: 0 779 1213 1236
Size: 779 434 23
Fragments arranged by size:
779 434 23
NarI GG'CG_CC
Cuts at: 0 795 1236
Size: 795 441
NcoI C'CATG_G
Cuts at: 0 774 1236
Size: 774 462
NsiI A_TGCA'T
Cuts at: 0 251 1236
Size: 251 985
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 1146 1236
Size: 339 807 90
Fragments arranged by size:
807 339 90
PfoI T'CCnGG_A
Cuts at: 0 713 1236
Size: 713 523
PpuMI rG'GwC_Cy
Cuts at: 0 204 1143 1181 1236
Size: 204 939 38 55
Fragments arranged by size:
939 204 55 38
PshAI GACnn'nnGTC
Cuts at: 0 190 1236
Size: 190 1046
SacI G_AGCT'C
Cuts at: 0 509 1236
Size: 509 727
SapI GCTCTTCn'nnn_
Cuts at: 0 514 1236
Size: 514 722
SfoI GGC'GCC
Cuts at: 0 796 1236
Size: 796 440
SmaI CCC'GGG
Cuts at: 0 229 872 1236
Size: 229 643 364
Fragments arranged by size:
643 364 229
SmlI C'TyrA_G
Cuts at: 0 141 799 1236
Size: 141 658 437
Fragments arranged by size:
658 437 141
StyI C'CwwG_G
Cuts at: 0 86 386 774 1236
Size: 86 300 388 462
Fragments arranged by size:
462 388 300 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 1236
Size: 289 947
TatI w'GTAC_w
Cuts at: 0 1091 1166 1236
Size: 1091 75 70
Fragments arranged by size:
1091 75 70
XbaI T'CTAG_A
Cuts at: 0 660 1236
Size: 660 576
XmaI C'CCGG_G
Cuts at: 0 227 870 1236
Size: 227 643 366
Fragments arranged by size:
643 366 227
XmnI GAAnn'nnTTC
Cuts at: 0 722 1236
Size: 722 514
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApoI AvaI BanI BanII
BbeI BcgI BfrBI BglII Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
KasI MmeI MslI NarI NcoI NsiI PflMI PfoI
PpuMI PshAI SacI SapI SfoI SmaI SmlI StyI
TaqII TatI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BglI
BlpI BmrI BmtI BsaI BsaAI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EcoNI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XcmI XhoI ZraI