Restriction map for
pFN21AE1878
(Linear) (Six-base) MAPSORT of: KIEE1878.seq Check: 1030 from: 1 to: 1194
KIEE1878.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1194
Size: 31 1163
AflIII A'CryG_T
Cuts at: 0 14 1194
Size: 14 1180
AhdI GACnn_n'nnGTC
Cuts at: 0 354 1194
Size: 354 840
AleI CACnn'nnGTG
Cuts at: 0 255 1194
Size: 255 939
AlwNI CAG_nnn'CTG
Cuts at: 0 70 80 1194
Size: 70 10 1114
Fragments arranged by size:
1114 70 10
ApaI G_GGCC'C
Cuts at: 0 264 1194
Size: 264 930
ApoI r'AATT_y
Cuts at: 0 419 469 544 1194
Size: 419 50 75 650
Fragments arranged by size:
650 419 75 50
AvaI C'yCGr_G
Cuts at: 0 855 1194
Size: 855 339
BanI G'GyrC_C
Cuts at: 0 146 156 310 1194
Size: 146 10 154 884
Fragments arranged by size:
884 154 146 10
BanII G_rGCy'C
Cuts at: 0 264 368 1194
Size: 264 104 826
Fragments arranged by size:
826 264 104
BclI T'GATC_A
Cuts at: 0 713 1194
Size: 713 481
Bme1580I G_kGCm'C
Cuts at: 0 151 161 264 1194
Size: 151 10 103 930
Fragments arranged by size:
930 151 103 10
BmgBI CAC'GTC
Cuts at: 0 492 1194
Size: 492 702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1194
Size: 712 482
BsaI GGTCTCn'nnnn_
Cuts at: 0 694 1194
Size: 694 500
BsaAI yAC'GTr
Cuts at: 0 15 1194
Size: 15 1179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 851 881 1194
Size: 851 30 313
Fragments arranged by size:
851 313 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1023 1194
Size: 1023 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 1194
Size: 306 888
BsiHKAI G_wGCw'C
Cuts at: 0 276 368 1194
Size: 276 92 826
Fragments arranged by size:
826 276 92
Bsp1286I G_dGCh'C
Cuts at: 0 151 161 264 276 368 1194
Size: 151 10 103 12 92 826
Fragments arranged by size:
826 151 103 92 12 10
BsrFI r'CCGG_y
Cuts at: 0 83 790 1194
Size: 83 707 404
Fragments arranged by size:
707 404 83
Bsu36I CC'TnA_GG
Cuts at: 0 142 1194
Size: 142 1052
DraI TTT'AAA
Cuts at: 0 972 1194
Size: 972 222
DraIII CAC_nnn'GTG
Cuts at: 0 588 1194
Size: 588 606
EaeI y'GGCC_r
Cuts at: 0 824 1194
Size: 824 370
EarI CTCTTCn'nnn_
Cuts at: 0 239 524 692 1194
Size: 239 285 168 502
Fragments arranged by size:
502 285 239 168
EcoICRI GAG'CTC
Cuts at: 0 366 1194
Size: 366 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1194
Size: 31 1163
EcoRI G'AATT_C
Cuts at: 0 469 1194
Size: 469 725
HaeII r_GCGC'y
Cuts at: 0 851 1194
Size: 851 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 749 781 921 953 1165 1194
Size: 340 32 377 32 140 32 212 29
Fragments arranged by size:
377 340 212 140 32 32 32 29
HincII GTy'rAC
Cuts at: 0 434 1194
Size: 434 760
MslI CAynn'nnrTG
Cuts at: 0 212 255 709 919 1194
Size: 212 43 454 210 275
Fragments arranged by size:
454 275 212 210 43
MspA1I CmG'CkG
Cuts at: 0 617 1194
Size: 617 577
PflMI CCAn_nnn'nTGG
Cuts at: 0 815 1194
Size: 815 379
PspOMI G'GGCC_C
Cuts at: 0 260 1194
Size: 260 934
SacI G_AGCT'C
Cuts at: 0 368 1194
Size: 368 826
SfcI C'TryA_G
Cuts at: 0 134 1194
Size: 134 1060
SmaI CCC'GGG
Cuts at: 0 857 1194
Size: 857 337
SmlI C'TyrA_G
Cuts at: 0 727 1194
Size: 727 467
SspI AAT'ATT
Cuts at: 0 892 1194
Size: 892 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 850 886 1194
Size: 850 36 308
Fragments arranged by size:
850 308 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 851 883 1194
Size: 851 32 311
Fragments arranged by size:
851 311 32
XmaI C'CCGG_G
Cuts at: 0 855 1194
Size: 855 339
XmnI GAAnn'nnTTC
Cuts at: 0 336 1194
Size: 336 858
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaI ApoI AvaI
BanI BanII BclI Bme1580I BmgBI BpuEI BsaI BsaAI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BsrFI Bsu36I DraI
DraIII EaeI EarI EcoICRI Eco57MI EcoRI HaeII Hin4I
HincII MslI MspA1I PflMI PspOMI SacI SfcI SmaI
SmlI SspI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmrI BmtI BplI BpmI Bpu10I BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DrdI EagI EciI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI StyI SwaI
TatI Tth111I XbaI XcmI XhoI ZraI