Restriction map for
pFN21AE1876
(Linear) (Six-base) MAPSORT of: KIEE1876.seq Check: 8551 from: 1 to: 1194
KIEE1876.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 258 1194
Size: 258 936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1194
Size: 583 611
AfeI AGC'GCT
Cuts at: 0 529 589 1194
Size: 529 60 605
Fragments arranged by size:
605 529 60
ApoI r'AATT_y
Cuts at: 0 567 1054 1194
Size: 567 487 140
Fragments arranged by size:
567 487 140
AvaI C'yCGr_G
Cuts at: 0 850 1194
Size: 850 344
BamHI G'GATC_C
Cuts at: 0 35 1011 1194
Size: 35 976 183
Fragments arranged by size:
976 183 35
BanI G'GyrC_C
Cuts at: 0 910 1194
Size: 910 284
BbeI G_GCGC'C
Cuts at: 0 914 1194
Size: 914 280
BbsI GAAGACnn'nnnn_
Cuts at: 0 280 1194
Size: 280 914
BclI T'GATC_A
Cuts at: 0 534 1194
Size: 534 660
BmrI ACTGGGnnnn_n'
Cuts at: 0 947 1194
Size: 947 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1194
Size: 1192 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1194
Size: 897 297
BsaI GGTCTCn'nnnn_
Cuts at: 0 947 1194
Size: 947 247
BsaBI GATnn'nnATC
Cuts at: 0 473 1194
Size: 473 721
BsaHI Gr'CG_yC
Cuts at: 0 911 1194
Size: 911 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 659 689 1194
Size: 193 30 436 30 505
Fragments arranged by size:
505 436 193 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 242 1194
Size: 242 952
BseYI C'CCAG_C
Cuts at: 0 984 1194
Size: 984 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 258 1194
Size: 258 936
BsrBI CCG'CTC
Cuts at: 0 1176 1194
Size: 1176 18
BsrDI GCAATG_nn'
Cuts at: 0 92 1194
Size: 92 1102
BssSI C'ACGA_G
Cuts at: 0 187 1194
Size: 187 1007
BstYI r'GATC_y
Cuts at: 0 35 498 1011 1194
Size: 35 463 513 183
Fragments arranged by size:
513 463 183 35
BtgI C'CryG_G
Cuts at: 0 240 782 1194
Size: 240 542 412
Fragments arranged by size:
542 412 240
EaeI y'GGCC_r
Cuts at: 0 167 779 1194
Size: 167 612 415
Fragments arranged by size:
612 415 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1192 1194
Size: 583 609 2
Fragments arranged by size:
609 583 2
EcoO109I rG'GnC_Cy
Cuts at: 0 74 1194
Size: 74 1120
EcoRV GAT'ATC
Cuts at: 0 370 1194
Size: 370 824
HaeII r_GCGC'y
Cuts at: 0 531 591 914 1194
Size: 531 60 323 280
Fragments arranged by size:
531 323 280 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 98 927 959 1194
Size: 66 32 829 32 235
Fragments arranged by size:
829 235 66 32 32
HindIII A'AGCT_T
Cuts at: 0 287 1194
Size: 287 907
KasI G'GCGC_C
Cuts at: 0 910 1194
Size: 910 284
MfeI C'AATT_G
Cuts at: 0 1089 1194
Size: 1089 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 699 1061 1194
Size: 26 673 362 133
Fragments arranged by size:
673 362 133 26
MslI CAynn'nnrTG
Cuts at: 0 431 1194
Size: 431 763
NarI GG'CG_CC
Cuts at: 0 911 1194
Size: 911 283
NcoI C'CATG_G
Cuts at: 0 240 1194
Size: 240 954
PasI CC'CwG_GG
Cuts at: 0 52 1194
Size: 52 1142
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 222 1194
Size: 45 177 972
Fragments arranged by size:
972 177 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 767 1126 1158 1194
Size: 735 32 359 32 36
Fragments arranged by size:
735 359 36 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 74 1194
Size: 74 1120
PstI C_TGCA'G
Cuts at: 0 509 1194
Size: 509 685
SanDI GG'GwC_CC
Cuts at: 0 74 1194
Size: 74 1120
SexAI A'CCwGG_T
Cuts at: 0 113 1194
Size: 113 1081
SfcI C'TryA_G
Cuts at: 0 505 1115 1194
Size: 505 610 79
Fragments arranged by size:
610 505 79
SfoI GGC'GCC
Cuts at: 0 912 1194
Size: 912 282
SmlI C'TyrA_G
Cuts at: 0 876 1194
Size: 876 318
StyI C'CwwG_G
Cuts at: 0 240 1194
Size: 240 954
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 471 1194
Size: 471 723
Tth111I GACn'n_nGTC
Cuts at: 0 939 1194
Size: 939 255
XmnI GAAnn'nnTTC
Cuts at: 0 277 568 1194
Size: 277 291 626
Fragments arranged by size:
626 291 277
Enzymes that do cut:
AarI AcuI AfeI ApoI AvaI BamHI BanI BbeI
BbsI BclI BmrI BpmI BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BspMI BsrBI BsrDI BssSI BstYI
BtgI EaeI Eco57MI EcoO109I EcoRV HaeII Hin4I HindIII
KasI MfeI MmeI MslI NarI NcoI PasI PflMI
PpiI PpuMI PstI SanDI SexAI SfcI SfoI SmlI
StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanII BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BsaAI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HincII HpaI KpnI
MluI MscI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI XbaI XcmI XhoI XmaI ZraI