Restriction map for
pFN21AE1869
(Linear) (Six-base) MAPSORT of: KIEE1869.seq Check: 6370 from: 1 to: 1170
KIEE1869.orf
With 182 enzymes: SgfI *
December 12, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 103 1170
Size: 103 1067
Acc65I G'GTAC_C
Cuts at: 0 429 1170
Size: 429 741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1170
Size: 840 330
AleI CACnn'nnGTG
Cuts at: 0 328 1170
Size: 328 842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 1170
Size: 571 599
AlwNI CAG_nnn'CTG
Cuts at: 0 159 810 1080 1170
Size: 159 651 270 90
Fragments arranged by size:
651 270 159 90
ApaI G_GGCC'C
Cuts at: 0 804 974 1170
Size: 804 170 196
Fragments arranged by size:
804 196 170
AvaI C'yCGr_G
Cuts at: 0 186 220 289 452 967 1041 1170
Size: 186 34 69 163 515 74 129
Fragments arranged by size:
515 186 163 129 74 69 34
BamHI G'GATC_C
Cuts at: 0 929 1170
Size: 929 241
BanI G'GyrC_C
Cuts at: 0 201 429 561 824 1045 1056 1170
Size: 201 228 132 263 221 11 114
Fragments arranged by size:
263 228 221 201 132 114 11
BanII G_rGCy'C
Cuts at: 0 290 419 804 837 941 974 1170
Size: 290 129 385 33 104 33 196
Fragments arranged by size:
385 290 196 129 104 33 33
BbeI G_GCGC'C
Cuts at: 0 1049 1060 1170
Size: 1049 11 110
Fragments arranged by size:
1049 110 11
BbvCI CC'TCA_GC
Cuts at: 0 472 544 1170
Size: 472 72 626
Fragments arranged by size:
626 472 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 121 897 931 1170
Size: 87 34 776 34 239
Fragments arranged by size:
776 239 87 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 12 72 1054 1170
Size: 12 60 982 116
Fragments arranged by size:
982 116 60 12
Bme1580I G_kGCm'C
Cuts at: 0 804 974 1170
Size: 804 170 196
Fragments arranged by size:
804 196 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1102 1170
Size: 814 288 68
Fragments arranged by size:
814 288 68
Bpu10I CC'TnA_GC
Cuts at: 0 472 544 655 1170
Size: 472 72 111 515
Fragments arranged by size:
515 472 111 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1170
Size: 463 707
BsaAI yAC'GTr
Cuts at: 0 501 1107 1170
Size: 501 606 63
Fragments arranged by size:
606 501 63
BsaHI Gr'CG_yC
Cuts at: 0 64 1046 1057 1170
Size: 64 982 11 113
Fragments arranged by size:
982 113 64 11
BsaWI w'CCGG_w
Cuts at: 0 282 601 1170
Size: 282 319 569
Fragments arranged by size:
569 319 282
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1120 1150 1170
Size: 1120 30 20
Fragments arranged by size:
1120 30 20
BseYI C'CCAG_C
Cuts at: 0 565 803 1170
Size: 565 238 367
Fragments arranged by size:
565 367 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1170
Size: 296 874
BsiEI CG_ry'CG
Cuts at: 0 76 80 258 726 1170
Size: 76 4 178 468 444
Fragments arranged by size:
468 444 178 76 4
BsiHKAI G_wGCw'C
Cuts at: 0 233 419 1170
Size: 233 186 751
Fragments arranged by size:
751 233 186
Bsp1286I G_dGCh'C
Cuts at: 0 233 290 419 804 837 941 974 1170
Size: 233 57 129 385 33 104 33 196
Fragments arranged by size:
385 233 196 129 104 57 33 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 1170
Size: 103 1067
BsrFI r'CCGG_y
Cuts at: 0 71 75 551 721 761 1170
Size: 71 4 476 170 40 409
Fragments arranged by size:
476 409 170 71 40 4
BsrGI T'GTAC_A
Cuts at: 0 239 893 1025 1170
Size: 239 654 132 145
Fragments arranged by size:
654 239 145 132
BssSI C'ACGA_G
Cuts at: 0 934 1170
Size: 934 236
BstEII G'GTnAC_C
Cuts at: 0 321 733 1170
Size: 321 412 437
Fragments arranged by size:
437 412 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 1170
Size: 792 378
BstYI r'GATC_y
Cuts at: 0 155 929 1170
Size: 155 774 241
Fragments arranged by size:
774 241 155
Bsu36I CC'TnA_GG
Cuts at: 0 296 1170
Size: 296 874
BtgI C'CryG_G
Cuts at: 0 80 147 627 863 1170
Size: 80 67 480 236 307
Fragments arranged by size:
480 307 236 80 67
BtsI GCAGTG_nn'
Cuts at: 0 622 663 691 1170
Size: 622 41 28 479
Fragments arranged by size:
622 479 41 28
EaeI y'GGCC_r
Cuts at: 0 69 73 77 150 263 723 740 782
Size: 69 4 4 73 113 460 17 42
Cuts at: 782 1170
Size: 388
Fragments arranged by size:
460 388 113 73 69 42 17 4
4
EagI C'GGCC_G
Cuts at: 0 73 77 723 1170
Size: 73 4 646 447
Fragments arranged by size:
646 447 73 4
EcoICRI GAG'CTC
Cuts at: 0 417 1170
Size: 417 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 840 1102 1170
Size: 814 26 262 68
Fragments arranged by size:
814 262 68 26
EcoO109I rG'GnC_Cy
Cuts at: 0 800 912 971 1014 1170
Size: 800 112 59 43 156
Fragments arranged by size:
800 156 112 59 43
FseI GG_CCGG'CC
Cuts at: 0 75 79 1170
Size: 75 4 1091
Fragments arranged by size:
1091 75 4
HaeII r_GCGC'y
Cuts at: 0 662 1049 1060 1081 1170
Size: 662 387 11 21 89
Fragments arranged by size:
662 387 89 21 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 1120 1152 1170
Size: 571 32 517 32 18
Fragments arranged by size:
571 517 32 32 18
KasI G'GCGC_C
Cuts at: 0 1045 1056 1170
Size: 1045 11 114
Fragments arranged by size:
1045 114 11
KpnI G_GTAC'C
Cuts at: 0 433 1170
Size: 433 737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1170
Size: 1163 7
MscI TGG'CCA
Cuts at: 0 742 784 1170
Size: 742 42 386
Fragments arranged by size:
742 386 42
MslI CAynn'nnrTG
Cuts at: 0 328 394 1147 1170
Size: 328 66 753 23
Fragments arranged by size:
753 328 66 23
MspA1I CmG'CkG
Cuts at: 0 42 82 149 216 792 891 1089 1134
Size: 42 40 67 67 576 99 198 45
Cuts at: 1134 1170
Size: 36
Fragments arranged by size:
576 198 99 67 67 45 42 40
36
NaeI GCC'GGC
Cuts at: 0 73 77 1170
Size: 73 4 1093
Fragments arranged by size:
1093 73 4
NarI GG'CG_CC
Cuts at: 0 1046 1057 1170
Size: 1046 11 113
Fragments arranged by size:
1046 113 11
NgoMIV G'CCGG_C
Cuts at: 0 71 75 1170
Size: 71 4 1095
Fragments arranged by size:
1095 71 4
PmlI CAC'GTG
Cuts at: 0 501 1170
Size: 501 669
PpuMI rG'GwC_Cy
Cuts at: 0 912 1014 1170
Size: 912 102 156
Fragments arranged by size:
912 156 102
PspOMI G'GGCC_C
Cuts at: 0 800 970 1170
Size: 800 170 200
Fragments arranged by size:
800 200 170
PvuII CAG'CTG
Cuts at: 0 891 1134 1170
Size: 891 243 36
Fragments arranged by size:
891 243 36
SacI G_AGCT'C
Cuts at: 0 419 1170
Size: 419 751
SacII CC_GC'GG
Cuts at: 0 83 150 1170
Size: 83 67 1020
Fragments arranged by size:
1020 83 67
SfoI GGC'GCC
Cuts at: 0 1047 1058 1170
Size: 1047 11 112
Fragments arranged by size:
1047 112 11
SmaI CCC'GGG
Cuts at: 0 454 1043 1170
Size: 454 589 127
Fragments arranged by size:
589 454 127
SmlI C'TyrA_G
Cuts at: 0 478 1170
Size: 478 692
StyI C'CwwG_G
Cuts at: 0 314 941 1121 1170
Size: 314 627 180 49
Fragments arranged by size:
627 314 180 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 579 1170
Size: 579 591
TatI w'GTAC_w
Cuts at: 0 239 379 893 1004 1025 1100 1170
Size: 239 140 514 111 21 75 70
Fragments arranged by size:
514 239 140 111 75 70 21
XmaI C'CCGG_G
Cuts at: 0 452 1041 1170
Size: 452 589 129
Fragments arranged by size:
589 452 129
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BglI Bme1580I
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EagI
EcoICRI Eco57MI EcoO109I FseI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV PmlI
PpuMI PspOMI PvuII SacI SacII SfoI SmaI SmlI
StyI TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BsaI BsaBI BseRI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BssHII BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoNI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI