Restriction map for
pFN21AE1863
(Linear) (Six-base) MAPSORT of: KIEE1863.seq Check: 868 from: 1 to: 1146
KIEE1863.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 69 145 1146
Size: 69 76 1001
Fragments arranged by size:
1001 76 69
AfeI AGC'GCT
Cuts at: 0 562 1146
Size: 562 584
AleI CACnn'nnGTG
Cuts at: 0 421 1146
Size: 421 725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 997 1146
Size: 997 149
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 1146
Size: 529 32 585
Fragments arranged by size:
585 529 32
AlwNI CAG_nnn'CTG
Cuts at: 0 471 1146
Size: 471 675
ApaI G_GGCC'C
Cuts at: 0 496 899 1146
Size: 496 403 247
Fragments arranged by size:
496 403 247
BanI G'GyrC_C
Cuts at: 0 118 375 1037 1146
Size: 118 257 662 109
Fragments arranged by size:
662 257 118 109
BanII G_rGCy'C
Cuts at: 0 44 89 289 496 524 644 815 832
Size: 44 45 200 207 28 120 171 17
Cuts at: 832 853 899 1146
Size: 21 46 247
Fragments arranged by size:
247 207 200 171 120 46 45 44
28 21 17
BbeI G_GCGC'C
Cuts at: 0 122 1146
Size: 122 1024
BbvCI CC'TCA_GC
Cuts at: 0 1046 1146
Size: 1046 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 609 643 1146
Size: 609 34 503
Fragments arranged by size:
609 503 34
BglI GCCn_nnn'nGGC
Cuts at: 0 403 1146
Size: 403 743
BglII A'GATC_T
Cuts at: 0 881 1146
Size: 881 265
BlpI GC'TnA_GC
Cuts at: 0 643 1146
Size: 643 503
Bme1580I G_kGCm'C
Cuts at: 0 496 899 1146
Size: 496 403 247
Fragments arranged by size:
496 403 247
BmgBI CAC'GTC
Cuts at: 0 540 1146
Size: 540 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 493 1146
Size: 46 447 653
Fragments arranged by size:
653 447 46
Bpu10I CC'TnA_GC
Cuts at: 0 1046 1146
Size: 1046 100
BsaHI Gr'CG_yC
Cuts at: 0 119 1146
Size: 119 1027
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2 32 424 454 691 715 721 739
Size: 2 30 392 30 237 24 6 18
Cuts at: 739 745 763 769 787 793 817 980 1010
Size: 6 18 6 18 6 24 163 30
Cuts at: 1010 1146
Size: 136
Fragments arranged by size:
392 237 163 136 30 30 30 24
24 18 18 18 6 6 6 6
2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 523 526 529 532 1146
Size: 34 3 486 3 3 3 614
Fragments arranged by size:
614 486 34 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 363 821 892 984 1105 1146
Size: 363 458 71 92 121 41
Fragments arranged by size:
458 363 121 92 71 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 229 340 523 655 1146
Size: 193 36 111 183 132 491
Fragments arranged by size:
491 193 183 132 111 36
BsiEI CG_ry'CG
Cuts at: 0 266 1146
Size: 266 880
BsiHKAI G_wGCw'C
Cuts at: 0 644 1146
Size: 644 502
BsmBI CGTCTCn'nnnn_
Cuts at: 0 363 1146
Size: 363 783
Bsp1286I G_dGCh'C
Cuts at: 0 44 89 289 496 524 644 815 832
Size: 44 45 200 207 28 120 171 17
Cuts at: 832 853 899 1146
Size: 21 46 247
Fragments arranged by size:
247 207 200 171 120 46 45 44
28 21 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 145 945 1146
Size: 69 76 800 201
Fragments arranged by size:
800 201 76 69
BsrFI r'CCGG_y
Cuts at: 0 96 1034 1120 1146
Size: 96 938 86 26
Fragments arranged by size:
938 96 86 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 1146
Size: 318 828
BstEII G'GTnAC_C
Cuts at: 0 382 943 1146
Size: 382 561 203
Fragments arranged by size:
561 382 203
BstYI r'GATC_y
Cuts at: 0 881 1146
Size: 881 265
BtgI C'CryG_G
Cuts at: 0 107 159 422 956 1146
Size: 107 52 263 534 190
Fragments arranged by size:
534 263 190 107 52
BtsI GCAGTG_nn'
Cuts at: 0 181 981 1146
Size: 181 800 165
Fragments arranged by size:
800 181 165
DraIII CAC_nnn'GTG
Cuts at: 0 318 1146
Size: 318 828
EaeI y'GGCC_r
Cuts at: 0 51 104 263 1122 1146
Size: 51 53 159 859 24
Fragments arranged by size:
859 159 53 51 24
EagI C'GGCC_G
Cuts at: 0 263 1146
Size: 263 883
EcoICRI GAG'CTC
Cuts at: 0 642 1146
Size: 642 504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 493 1146
Size: 46 447 653
Fragments arranged by size:
653 447 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1045 1146
Size: 1045 101
EcoO109I rG'GnC_Cy
Cuts at: 0 164 183 269 584 1146
Size: 164 19 86 315 562
Fragments arranged by size:
562 315 164 86 19
FspI TGC'GCA
Cuts at: 0 244 1146
Size: 244 902
HaeII r_GCGC'y
Cuts at: 0 122 564 1146
Size: 122 442 582
Fragments arranged by size:
582 442 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 691 715 723 739 747 763
Size: 2 32 657 24 8 16 8 16
Cuts at: 763 771 787 795 819 899 931 980 1012
Size: 8 16 8 24 80 32 49 32
Cuts at: 1012 1146
Size: 134
Fragments arranged by size:
657 134 80 49 32 32 32 24
24 16 16 16 8 8 8 8
2
KasI G'GCGC_C
Cuts at: 0 118 1146
Size: 118 1028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1146
Size: 194 952
MslI CAynn'nnrTG
Cuts at: 0 421 1146
Size: 421 725
MspA1I CmG'CkG
Cuts at: 0 161 351 951 958 1146
Size: 161 190 600 7 188
Fragments arranged by size:
600 190 188 161 7
NaeI GCC'GGC
Cuts at: 0 1036 1146
Size: 1036 110
NarI GG'CG_CC
Cuts at: 0 119 1146
Size: 119 1027
NgoMIV G'CCGG_C
Cuts at: 0 1034 1146
Size: 1034 112
PasI CC'CwG_GG
Cuts at: 0 489 525 579 1146
Size: 489 36 54 567
Fragments arranged by size:
567 489 54 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 1146
Size: 471 675
PfoI T'CCnGG_A
Cuts at: 0 341 1146
Size: 341 805
PpuMI rG'GwC_Cy
Cuts at: 0 269 584 1146
Size: 269 315 562
Fragments arranged by size:
562 315 269
PshAI GACnn'nnGTC
Cuts at: 0 589 1146
Size: 589 557
PspOMI G'GGCC_C
Cuts at: 0 492 895 1146
Size: 492 403 251
Fragments arranged by size:
492 403 251
PstI C_TGCA'G
Cuts at: 0 80 1146
Size: 80 1066
PvuII CAG'CTG
Cuts at: 0 351 951 1146
Size: 351 600 195
Fragments arranged by size:
600 351 195
RsrII CG'GwC_CG
Cuts at: 0 156 1146
Size: 156 990
SacI G_AGCT'C
Cuts at: 0 644 1146
Size: 644 502
SacII CC_GC'GG
Cuts at: 0 162 959 1146
Size: 162 797 187
Fragments arranged by size:
797 187 162
SanDI GG'GwC_CC
Cuts at: 0 269 584 1146
Size: 269 315 562
Fragments arranged by size:
562 315 269
SbfI CC_TGCA'GG
Cuts at: 0 80 1146
Size: 80 1066
SfcI C'TryA_G
Cuts at: 0 76 1146
Size: 76 1070
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 403 1146
Size: 403 743
SfoI GGC'GCC
Cuts at: 0 120 1146
Size: 120 1026
StyI C'CwwG_G
Cuts at: 0 113 1146
Size: 113 1033
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1072 1076 1146
Size: 1072 4 70
Fragments arranged by size:
1072 70 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 960 992 1146
Size: 960 32 154
Fragments arranged by size:
960 154 32
Enzymes that do cut:
AarI AfeI AleI AlfI AloI AlwNI ApaI BanI
BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstEII
BstYI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII RsrII SacI SacII SanDI
SbfI SfcI SfiI SfoI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EarI EciI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI SalI SapI
ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI