Restriction map for
pFN21AE1862
(Linear) (Six-base) MAPSORT of: KIEE1862.seq Check: 7734 from: 1 to: 1143
KIEE1862.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AatII G_ACGT'C
Cuts at: 0 239 1143
Size: 239 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1143
Size: 794 349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 235 1143
Size: 26 209 908
Fragments arranged by size:
908 209 26
AlwNI CAG_nnn'CTG
Cuts at: 0 261 1143
Size: 261 882
ApoI r'AATT_y
Cuts at: 0 84 822 1143
Size: 84 738 321
Fragments arranged by size:
738 321 84
AscI GG'CGCG_CC
Cuts at: 0 438 1143
Size: 438 705
BanI G'GyrC_C
Cuts at: 0 814 1143
Size: 814 329
BanII G_rGCy'C
Cuts at: 0 321 839 1131 1143
Size: 321 518 292 12
Fragments arranged by size:
518 321 292 12
BfrBI ATG'CAT
Cuts at: 0 3 1143
Size: 3 1140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1143
Size: 208 935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1143
Size: 631 512
BsaI GGTCTCn'nnnn_
Cuts at: 0 591 1042 1143
Size: 591 451 101
Fragments arranged by size:
591 451 101
BsaBI GATnn'nnATC
Cuts at: 0 68 1143
Size: 68 1075
BsaHI Gr'CG_yC
Cuts at: 0 236 1143
Size: 236 907
BsaWI w'CCGG_w
Cuts at: 0 1051 1143
Size: 1051 92
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 442 475 769 1070 1139 1143
Size: 283 159 33 294 301 69 4
Fragments arranged by size:
301 294 283 159 69 33 4
BseYI C'CCAG_C
Cuts at: 0 273 503 1143
Size: 273 230 640
Fragments arranged by size:
640 273 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 517 1143
Size: 472 45 626
Fragments arranged by size:
626 472 45
BsiHKAI G_wGCw'C
Cuts at: 0 30 158 348 1131 1143
Size: 30 128 190 783 12
Fragments arranged by size:
783 190 128 30 12
Bsp1286I G_dGCh'C
Cuts at: 0 30 158 321 348 839 1131 1143
Size: 30 128 163 27 491 292 12
Fragments arranged by size:
491 292 163 128 30 27 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 520 1143
Size: 494 26 623
Fragments arranged by size:
623 494 26
BsrDI GCAATG_nn'
Cuts at: 0 1038 1143
Size: 1038 105
BsrFI r'CCGG_y
Cuts at: 0 278 525 1143
Size: 278 247 618
Fragments arranged by size:
618 278 247
BssHII G'CGCG_C
Cuts at: 0 308 438 1143
Size: 308 130 705
Fragments arranged by size:
705 308 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 495 1143
Size: 261 234 648
Fragments arranged by size:
648 261 234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 1120 1143
Size: 348 772 23
Fragments arranged by size:
772 348 23
BstYI r'GATC_y
Cuts at: 0 1109 1143
Size: 1109 34
Bsu36I CC'TnA_GG
Cuts at: 0 908 1143
Size: 908 235
BtgI C'CryG_G
Cuts at: 0 227 449 779 1143
Size: 227 222 330 364
Fragments arranged by size:
364 330 227 222
BtsI GCAGTG_nn'
Cuts at: 0 47 1143
Size: 47 1096
DraIII CAC_nnn'GTG
Cuts at: 0 137 495 1143
Size: 137 358 648
Fragments arranged by size:
648 358 137
EaeI y'GGCC_r
Cuts at: 0 47 446 887 930 1143
Size: 47 399 441 43 213
Fragments arranged by size:
441 399 213 47 43
EarI CTCTTCn'nnn_
Cuts at: 0 181 1031 1143
Size: 181 850 112
Fragments arranged by size:
850 181 112
EcoICRI GAG'CTC
Cuts at: 0 1129 1143
Size: 1129 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 794 1143
Size: 208 586 349
Fragments arranged by size:
586 349 208
EcoNI CCTnn'n_nnAGG
Cuts at: 0 911 1143
Size: 911 232
EcoO109I rG'GnC_Cy
Cuts at: 0 126 1143
Size: 126 1017
EcoRI G'AATT_C
Cuts at: 0 822 1143
Size: 822 321
HaeII r_GCGC'y
Cuts at: 0 423 1143
Size: 423 720
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 330 362 1143
Size: 330 32 781
Fragments arranged by size:
781 330 32
MscI TGG'CCA
Cuts at: 0 889 932 1143
Size: 889 43 211
Fragments arranged by size:
889 211 43
MslI CAynn'nnrTG
Cuts at: 0 79 846 1143
Size: 79 767 297
Fragments arranged by size:
767 297 79
MspA1I CmG'CkG
Cuts at: 0 451 711 1143
Size: 451 260 432
Fragments arranged by size:
451 432 260
NaeI GCC'GGC
Cuts at: 0 280 527 1143
Size: 280 247 616
Fragments arranged by size:
616 280 247
NcoI C'CATG_G
Cuts at: 0 779 1143
Size: 779 364
NdeI CA'TA_TG
Cuts at: 0 699 1143
Size: 699 444
NgoMIV G'CCGG_C
Cuts at: 0 278 525 1143
Size: 278 247 618
Fragments arranged by size:
618 278 247
NsiI A_TGCA'T
Cuts at: 0 5 1143
Size: 5 1138
NspI r_CATG'y
Cuts at: 0 216 1143
Size: 216 927
PasI CC'CwG_GG
Cuts at: 0 789 1143
Size: 789 354
PpuMI rG'GwC_Cy
Cuts at: 0 126 1143
Size: 126 1017
PstI C_TGCA'G
Cuts at: 0 42 257 365 926 1143
Size: 42 215 108 561 217
Fragments arranged by size:
561 217 215 108 42
PvuII CAG'CTG
Cuts at: 0 711 1143
Size: 711 432
SacI G_AGCT'C
Cuts at: 0 1131 1143
Size: 1131 12
SacII CC_GC'GG
Cuts at: 0 452 1143
Size: 452 691
SapI GCTCTTCn'nnn_
Cuts at: 0 181 1143
Size: 181 962
SfcI C'TryA_G
Cuts at: 0 38 253 361 922 1143
Size: 38 215 108 561 221
Fragments arranged by size:
561 221 215 108 38
SmlI C'TyrA_G
Cuts at: 0 610 1143
Size: 610 533
SphI G_CATG'C
Cuts at: 0 216 1143
Size: 216 927
SspI AAT'ATT
Cuts at: 0 1086 1143
Size: 1086 57
StyI C'CwwG_G
Cuts at: 0 779 1143
Size: 779 364
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 736 946 1082 1143
Size: 736 210 136 61
Fragments arranged by size:
736 210 136 61
Tth111I GACn'n_nGTC
Cuts at: 0 235 1143
Size: 235 908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 738 786 882 940 1143
Size: 738 48 96 58 203
Fragments arranged by size:
738 203 96 58 48
ZraI GAC'GTC
Cuts at: 0 237 1143
Size: 237 906
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApoI AscI BanI BanII
BfrBI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI
NspI PasI PpuMI PstI PvuII SacI SacII SapI
SfcI SmlI SphI SspI StyI TaqII Tth111I XcmI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NheI NotI NruI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI TatI TstI XbaI
XhoI XmaI XmnI