Restriction map for
pFN21AE1859
(Linear) (Six-base) MAPSORT of: KIEE1859.seq Check: 8209 from: 1 to: 1137
KIEE1859.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1137
Size: 415 722
ApoI r'AATT_y
Cuts at: 0 283 559 634 1137
Size: 283 276 75 503
Fragments arranged by size:
503 283 276 75
AvaI C'yCGr_G
Cuts at: 0 46 359 1137
Size: 46 313 778
Fragments arranged by size:
778 313 46
BanI G'GyrC_C
Cuts at: 0 231 1137
Size: 231 906
BanII G_rGCy'C
Cuts at: 0 47 1137
Size: 47 1090
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 998 1137
Size: 965 33 139
Fragments arranged by size:
965 139 33
BsaBI GATnn'nnATC
Cuts at: 0 224 1137
Size: 224 913
BsaHI Gr'CG_yC
Cuts at: 0 142 1137
Size: 142 995
BsaWI w'CCGG_w
Cuts at: 0 228 407 1137
Size: 228 179 730
Fragments arranged by size:
730 228 179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 1137
Size: 166 30 941
Fragments arranged by size:
941 166 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1137
Size: 493 644
BseYI C'CCAG_C
Cuts at: 0 71 92 127 1137
Size: 71 21 35 1010
Fragments arranged by size:
1010 71 35 21
BsiEI CG_ry'CG
Cuts at: 0 122 1137
Size: 122 1015
Bsp1286I G_dGCh'C
Cuts at: 0 47 1137
Size: 47 1090
BspEI T'CCGG_A
Cuts at: 0 407 1137
Size: 407 730
BsrFI r'CCGG_y
Cuts at: 0 54 1137
Size: 54 1083
BsrGI T'GTAC_A
Cuts at: 0 194 1128 1137
Size: 194 934 9
Fragments arranged by size:
934 194 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 1137
Size: 258 879
BstYI r'GATC_y
Cuts at: 0 225 438 540 1081 1137
Size: 225 213 102 541 56
Fragments arranged by size:
541 225 213 102 56
BtgI C'CryG_G
Cuts at: 0 137 149 257 1137
Size: 137 12 108 880
Fragments arranged by size:
880 137 108 12
BtsI GCAGTG_nn'
Cuts at: 0 464 1137
Size: 464 673
DraI TTT'AAA
Cuts at: 0 639 1137
Size: 639 498
EaeI y'GGCC_r
Cuts at: 0 5 119 1137
Size: 5 114 1018
Fragments arranged by size:
1018 114 5
EagI C'GGCC_G
Cuts at: 0 119 1137
Size: 119 1018
EarI CTCTTCn'nnn_
Cuts at: 0 104 1137
Size: 104 1033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1137
Size: 415 722
EcoRV GAT'ATC
Cuts at: 0 951 1137
Size: 951 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1015 1137
Size: 1015 122
FspI TGC'GCA
Cuts at: 0 32 1137
Size: 32 1105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1137
Size: 166 32 939
Fragments arranged by size:
939 166 32
NaeI GCC'GGC
Cuts at: 0 56 1137
Size: 56 1081
NcoI C'CATG_G
Cuts at: 0 149 1137
Size: 149 988
NgoMIV G'CCGG_C
Cuts at: 0 54 1137
Size: 54 1083
NspI r_CATG'y
Cuts at: 0 966 1137
Size: 966 171
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 703 735 1137
Size: 703 32 402
Fragments arranged by size:
703 402 32
PstI C_TGCA'G
Cuts at: 0 323 662 1137
Size: 323 339 475
Fragments arranged by size:
475 339 323
SfcI C'TryA_G
Cuts at: 0 319 658 1137
Size: 319 339 479
Fragments arranged by size:
479 339 319
SmaI CCC'GGG
Cuts at: 0 48 361 1137
Size: 48 313 776
Fragments arranged by size:
776 313 48
SpeI A'CTAG_T
Cuts at: 0 804 1137
Size: 804 333
SrfI GCCC'GGGC
Cuts at: 0 48 1137
Size: 48 1089
SspI AAT'ATT
Cuts at: 0 1063 1137
Size: 1063 74
StyI C'CwwG_G
Cuts at: 0 149 1137
Size: 149 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 1137
Size: 95 1042
TatI w'GTAC_w
Cuts at: 0 194 1128 1137
Size: 194 934 9
Fragments arranged by size:
934 194 9
XmaI C'CCGG_G
Cuts at: 0 46 359 1137
Size: 46 313 778
Fragments arranged by size:
778 313 46
XmnI GAAnn'nnTTC
Cuts at: 0 995 1137
Size: 995 142
Enzymes that do cut:
AcuI ApoI AvaI BanI BanII BbsI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI Bsp1286I BspEI BsrFI
BsrGI BstXI BstYI BtgI BtsI DraI EaeI EagI
EarI Eco57MI EcoRV FalI FspI Hin4I NaeI NcoI
NgoMIV NspI PsrI PstI SfcI SmaI SpeI SrfI
SspI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsgI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EciI EcoICRI
EcoNI EcoO109I EcoRI FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SphI StuI SwaI
TstI Tth111I XbaI XcmI XhoI ZraI