Restriction map for
pFN21AE1858
(Linear) (Six-base) MAPSORT of: KIEE1858.seq Check: 2792 from: 1 to: 1137
KIEE1858.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 395 748 1137
Size: 81 314 353 389
Fragments arranged by size:
389 353 314 81
AflII C'TTAA_G
Cuts at: 0 634 1137
Size: 634 503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1044 1054 1137
Size: 1044 10 83
Fragments arranged by size:
1044 83 10
AlwNI CAG_nnn'CTG
Cuts at: 0 216 1137
Size: 216 921
ApoI r'AATT_y
Cuts at: 0 428 520 607 1050 1137
Size: 428 92 87 443 87
Fragments arranged by size:
443 428 92 87 87
AvrII C'CTAG_G
Cuts at: 0 870 1137
Size: 870 267
BanII G_rGCy'C
Cuts at: 0 16 677 1137
Size: 16 661 460
Fragments arranged by size:
661 460 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 394 1137
Size: 394 743
BlpI GC'TnA_GC
Cuts at: 0 10 15 676 1137
Size: 10 5 661 461
Fragments arranged by size:
661 461 10 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 863 1137
Size: 863 274
BsaWI w'CCGG_w
Cuts at: 0 432 1137
Size: 432 705
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1137
Size: 685 452
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1137
Size: 898 239
BsiHKAI G_wGCw'C
Cuts at: 0 16 677 1137
Size: 16 661 460
Fragments arranged by size:
661 460 16
BsmI GAATG_Cn'
Cuts at: 0 225 549 1137
Size: 225 324 588
Fragments arranged by size:
588 324 225
Bsp1286I G_dGCh'C
Cuts at: 0 16 677 1137
Size: 16 661 460
Fragments arranged by size:
661 460 16
BspEI T'CCGG_A
Cuts at: 0 432 1137
Size: 432 705
BspHI T'CATG_A
Cuts at: 0 524 1137
Size: 524 613
BstYI r'GATC_y
Cuts at: 0 882 1137
Size: 882 255
Bsu36I CC'TnA_GG
Cuts at: 0 1098 1137
Size: 1098 39
BtgI C'CryG_G
Cuts at: 0 119 719 1137
Size: 119 600 418
Fragments arranged by size:
600 418 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1002 1137
Size: 1002 135
BtsI GCAGTG_nn'
Cuts at: 0 1055 1137
Size: 1055 82
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 219 254 1137
Size: 219 35 883
Fragments arranged by size:
883 219 35
EaeI y'GGCC_r
Cuts at: 0 116 347 1137
Size: 116 231 790
Fragments arranged by size:
790 231 116
EarI CTCTTCn'nnn_
Cuts at: 0 185 830 977 1137
Size: 185 645 147 160
Fragments arranged by size:
645 185 160 147
EcoICRI GAG'CTC
Cuts at: 0 14 675 1137
Size: 14 661 462
Fragments arranged by size:
661 462 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 395 748 1137
Size: 81 314 353 389
Fragments arranged by size:
389 353 314 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 662 1137
Size: 662 475
EcoO109I rG'GnC_Cy
Cuts at: 0 630 1137
Size: 630 507
EcoRV GAT'ATC
Cuts at: 0 228 1137
Size: 228 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 1137
Size: 700 32 405
Fragments arranged by size:
700 405 32
HincII GTy'rAC
Cuts at: 0 749 1137
Size: 749 388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1137
Size: 881 256
MscI TGG'CCA
Cuts at: 0 118 349 1137
Size: 118 231 788
Fragments arranged by size:
788 231 118
MslI CAynn'nnrTG
Cuts at: 0 1036 1137
Size: 1036 101
MspA1I CmG'CkG
Cuts at: 0 9 159 1003 1137
Size: 9 150 844 134
Fragments arranged by size:
844 150 134 9
NcoI C'CATG_G
Cuts at: 0 119 1137
Size: 119 1018
NdeI CA'TA_TG
Cuts at: 0 333 615 1137
Size: 333 282 522
Fragments arranged by size:
522 333 282
NspI r_CATG'y
Cuts at: 0 370 921 1035 1137
Size: 370 551 114 102
Fragments arranged by size:
551 370 114 102
PpuMI rG'GwC_Cy
Cuts at: 0 630 1137
Size: 630 507
PvuII CAG'CTG
Cuts at: 0 9 159 1003 1137
Size: 9 150 844 134
Fragments arranged by size:
844 150 134 9
SacI G_AGCT'C
Cuts at: 0 16 677 1137
Size: 16 661 460
Fragments arranged by size:
661 460 16
SmlI C'TyrA_G
Cuts at: 0 634 1137
Size: 634 503
SphI G_CATG'C
Cuts at: 0 370 1137
Size: 370 767
StyI C'CwwG_G
Cuts at: 0 35 119 663 870 1137
Size: 35 84 544 207 267
Fragments arranged by size:
544 267 207 84 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 1137
Size: 622 32 483
Fragments arranged by size:
622 483 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 828 1137
Size: 126 702 309
Fragments arranged by size:
702 309 126
XmnI GAAnn'nnTTC
Cuts at: 0 194 1137
Size: 194 943
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApoI AvrII BanII BbsI
BlpI BplI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BstYI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I
HincII MmeI MscI MslI MspA1I NcoI NdeI NspI
PpuMI PvuII SacI SmlI SphI StyI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseYI BsiEI BsiWI BsmBI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
ClaI DraI DraIII DrdI EagI EciI EcoRI FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI ZraI