(Linear) (Six-base) MAPSORT of: KIEE1856.seq  Check: 7125  from: 1  to: 1125



KIEE1856.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      45    1125 
   Size:         45    1080
 
AatII G_ACGT'C

Cuts at:      0     557    1125 
   Size:        557     568
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     487     544    1125 
   Size:         82     405      57     581

  Fragments arranged by size: 

                 581     405      82      57
 
AflIII A'CryG_T

Cuts at:      0     288    1125 
   Size:        288     837
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     635     667    1125 
   Size:        635      32     458

  Fragments arranged by size: 

                 635     458      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60    1125 
   Size:         60    1065
 
ApoI r'AATT_y

Cuts at:      0     933    1125 
   Size:        933     192
 
BclI T'GATC_A

Cuts at:      0     959    1125 
   Size:        959     166
 
BmgBI CAC'GTC

Cuts at:      0      36    1125 
   Size:         36    1089
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1013    1115    1125 
   Size:         64     949     102      10

  Fragments arranged by size: 

                 949     102      64      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     197    1125 
   Size:        197     928
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     932    1125 
   Size:        932     193
 
BsaAI yAC'GTr

Cuts at:      0     291    1125 
   Size:        291     834
 
BsaHI Gr'CG_yC

Cuts at:      0     554    1125 
   Size:        554     571
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1125 
   Size:         58    1067
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      45    1125 
   Size:         45    1080
 
BsrFI r'CCGG_y

Cuts at:      0     111    1125 
   Size:        111    1014
 
BsrGI T'GTAC_A

Cuts at:      0     147    1125 
   Size:        147     978
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     251    1125 
   Size:        251     874
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     274    1125 
   Size:        274     851
 
BstYI r'GATC_y

Cuts at:      0     225    1032    1125 
   Size:        225     807      93

  Fragments arranged by size: 

                 807     225      93
 
BtsI GCAGTG_nn'

Cuts at:      0     692    1125 
   Size:        692     433
 
DraIII CAC_nnn'GTG

Cuts at:      0      13    1125 
   Size:         13    1112
 
EarI CTCTTCn'nnn_

Cuts at:      0      58     781    1046    1125 
   Size:         58     723     265      79

  Fragments arranged by size: 

                 723     265      79      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64      82     487     544    1013    1115    1125 
   Size:         64      18     405      57     469     102      10

  Fragments arranged by size: 

                 469     405     102      64      57      18      10
 
EcoRI G'AATT_C

Cuts at:      0     933    1125 
   Size:        933     192
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     210     242    1125 
   Size:        210      32     883

  Fragments arranged by size: 

                 883     210      32
 
HindIII A'AGCT_T

Cuts at:      0    1042    1125 
   Size:       1042      83
 
MfeI C'AATT_G

Cuts at:      0     968    1125 
   Size:        968     157
 
MspA1I CmG'CkG

Cuts at:      0     270    1125 
   Size:        270     855
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     991    1125 
   Size:        991     134
 
PfoI T'CCnGG_A

Cuts at:      0     128    1125 
   Size:        128     997
 
PmlI CAC'GTG

Cuts at:      0     291    1125 
   Size:        291     834
 
PvuII CAG'CTG

Cuts at:      0     270    1125 
   Size:        270     855
 
SmlI C'TyrA_G

Cuts at:      0     947    1125 
   Size:        947     178
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     610    1125 
   Size:        610     515
 
TatI w'GTAC_w

Cuts at:      0     147     258    1125 
   Size:        147     111     867

  Fragments arranged by size: 

                 867     147     111
 
XbaI T'CTAG_A

Cuts at:      0     204    1125 
   Size:        204     921
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318    1125 
   Size:        318     807
 
ZraI GAC'GTC

Cuts at:      0     555    1125 
   Size:        555     570
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AloI    AlwNI     ApoI     BclI
    BmgBI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI     BsgI    BspMI
    BsrFI    BsrGI   BstAPI    BstXI    BstYI     BtsI   DraIII     EarI
  Eco57MI    EcoRI    Hin4I  HindIII     MfeI   MspA1I    PflMI     PfoI
     PmlI    PvuII     SmlI    TaqII     TatI     XbaI     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XhoI     XmaI     XmnI