(Linear) (Six-base) MAPSORT of: KIEE1845.seq  Check: 7306  from: 1  to: 1083



KIEE1845.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     737    1083 
   Size:        424     313     346

  Fragments arranged by size: 

                 424     346     313
 
AfeI AGC'GCT

Cuts at:      0      65    1083 
   Size:         65    1018
 
AlwNI CAG_nnn'CTG

Cuts at:      0     161    1005    1083 
   Size:        161     844      78

  Fragments arranged by size: 

                 844     161      78
 
ApoI r'AATT_y

Cuts at:      0     569    1083 
   Size:        569     514
 
AvaI C'yCGr_G

Cuts at:      0      20     285     829    1083 
   Size:         20     265     544     254

  Fragments arranged by size: 

                 544     265     254      20
 
BanI G'GyrC_C

Cuts at:      0     834    1083 
   Size:        834     249
 
BanII G_rGCy'C

Cuts at:      0     292     554    1083 
   Size:        292     262     529

  Fragments arranged by size: 

                 529     292     262
 
BglI GCCn_nnn'nGGC

Cuts at:      0     961    1083 
   Size:        961     122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1027    1083 
   Size:        901     126      56

  Fragments arranged by size: 

                 901     126      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604    1042    1083 
   Size:        604     438      41

  Fragments arranged by size: 

                 604     438      41
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     276    1083 
   Size:        276     807
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     980    1083 
   Size:        980     103
 
BsiEI CG_ry'CG

Cuts at:      0      59    1083 
   Size:         59    1024
 
BsiHKAI G_wGCw'C

Cuts at:      0     356     554    1083 
   Size:        356     198     529

  Fragments arranged by size: 

                 529     356     198
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     288    1083 
   Size:        288     795
 
Bsp1286I G_dGCh'C

Cuts at:      0     292     356     554    1083 
   Size:        292      64     198     529

  Fragments arranged by size: 

                 529     292     198      64
 
BsrBI CCG'CTC

Cuts at:      0     206    1083 
   Size:        206     877
 
BsrFI r'CCGG_y

Cuts at:      0     193    1083 
   Size:        193     890
 
BstYI r'GATC_y

Cuts at:      0     646    1083 
   Size:        646     437
 
BtsI GCAGTG_nn'

Cuts at:      0     114     451    1083 
   Size:        114     337     632

  Fragments arranged by size: 

                 632     337     114
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     162     197    1083 
   Size:        162      35     886

  Fragments arranged by size: 

                 886     162      35
 
EaeI y'GGCC_r

Cuts at:      0      56      95    1083 
   Size:         56      39     988

  Fragments arranged by size: 

                 988      56      39
 
EagI C'GGCC_G

Cuts at:      0      56    1083 
   Size:         56    1027
 
EarI CTCTTCn'nnn_

Cuts at:      0     279     611    1083 
   Size:        279     332     472

  Fragments arranged by size: 

                 472     332     279
 
EcoICRI GAG'CTC

Cuts at:      0     552    1083 
   Size:        552     531
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     737     901    1027    1083 
   Size:        424     313     164     126      56

  Fragments arranged by size: 

                 424     313     164     126      56
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     454    1083 
   Size:        454     629
 
HaeII r_GCGC'y

Cuts at:      0      67     137     252    1083 
   Size:         67      70     115     831

  Fragments arranged by size: 

                 831     115      70      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     541     573    1083 
   Size:        541      32     510

  Fragments arranged by size: 

                 541     510      32
 
MscI TGG'CCA

Cuts at:      0      97    1083 
   Size:         97     986
 
MspA1I CmG'CkG

Cuts at:      0     101     158     215     823    1083 
   Size:        101      57      57     608     260

  Fragments arranged by size: 

                 608     260     101      57      57
 
NotI GC'GGCC_GC

Cuts at:      0      56    1083 
   Size:         56    1027
 
PvuII CAG'CTG

Cuts at:      0     158     215     823    1083 
   Size:        158      57     608     260

  Fragments arranged by size: 

                 608     260     158      57
 
SacI G_AGCT'C

Cuts at:      0     554    1083 
   Size:        554     529
 
SgrAI Cr'CCGG_yG

Cuts at:      0     193    1083 
   Size:        193     890
 
SmaI CCC'GGG

Cuts at:      0      22    1083 
   Size:         22    1061
 
SmlI C'TyrA_G

Cuts at:      0     583    1021    1083 
   Size:        583     438      62

  Fragments arranged by size: 

                 583     438      62
 
TatI w'GTAC_w

Cuts at:      0     759    1083 
   Size:        759     324
 
Tth111I GACn'n_nGTC

Cuts at:      0     390    1083 
   Size:        390     693
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     859    1083 
   Size:        859     224
 
XmaI C'CCGG_G

Cuts at:      0      20    1083 
   Size:         20    1063
 
XmnI GAAnn'nnTTC

Cuts at:      0     437     983    1083 
   Size:        437     546     100

  Fragments arranged by size: 

                 546     437     100
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     ApoI     AvaI     BanI    BanII     BglI
     BpmI    BpuEI    BseRI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BsrBI    BsrFI    BstYI     BtsI    CspCI     EaeI     EagI     EarI
  EcoICRI  Eco57MI     FalI    HaeII    Hin4I     MscI   MspA1I     NotI
    PvuII     SacI    SgrAI     SmaI     SmlI     TatI  Tth111I     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI     XbaI
     XhoI     ZraI