(Linear) (Six-base) MAPSORT of: KIEE1841.seq  Check: 298  from: 1  to: 1071



KIEE1841.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:48  ..

AccI GT'mk_AC

Cuts at:      0     181    1025    1071 
   Size:        181     844      46

  Fragments arranged by size: 

                 844     181      46
 
AclI AA'CG_TT

Cuts at:      0     777    1071 
   Size:        777     294
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     893    1071 
   Size:        893     178
 
AflIII A'CryG_T

Cuts at:      0     586    1071 
   Size:        586     485
 
ApaI G_GGCC'C

Cuts at:      0     195    1071 
   Size:        195     876
 
ApoI r'AATT_y

Cuts at:      0     616     993    1071 
   Size:        616     377      78

  Fragments arranged by size: 

                 616     377      78
 
AseI AT'TA_AT

Cuts at:      0     623    1071 
   Size:        623     448
 
BanII G_rGCy'C

Cuts at:      0     137     195    1071 
   Size:        137      58     876

  Fragments arranged by size: 

                 876     137      58
 
Bme1580I G_kGCm'C

Cuts at:      0     195    1071 
   Size:        195     876
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253    1071 
   Size:        253     818
 
BsaWI w'CCGG_w

Cuts at:      0     176    1071 
   Size:        176     895
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     964     994    1071 
   Size:        964      30      77

  Fragments arranged by size: 

                 964      77      30
 
BseYI C'CCAG_C

Cuts at:      0     196    1071 
   Size:        196     875
 
BsiHKAI G_wGCw'C

Cuts at:      0     484    1071 
   Size:        484     587
 
BsmI GAATG_Cn'

Cuts at:      0     278    1071 
   Size:        278     793
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     195     484    1071 
   Size:        137      58     289     587

  Fragments arranged by size: 

                 587     289     137      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     869    1071 
   Size:        869     202
 
BstBI TT'CG_AA

Cuts at:      0     381    1071 
   Size:        381     690
 
BstYI r'GATC_y

Cuts at:      0     575    1071 
   Size:        575     496
 
BtgI C'CryG_G

Cuts at:      0     116     485    1071 
   Size:        116     369     586

  Fragments arranged by size: 

                 586     369     116
 
BtsI GCAGTG_nn'

Cuts at:      0     610    1071 
   Size:        610     461
 
DraI TTT'AAA

Cuts at:      0     953    1071 
   Size:        953     118
 
EaeI y'GGCC_r

Cuts at:      0      36     318     939    1071 
   Size:         36     282     621     132

  Fragments arranged by size: 

                 621     282     132      36
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     566    1071 
   Size:         89     477     505

  Fragments arranged by size: 

                 505     477      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     893    1071 
   Size:        893     178
 
EcoO109I rG'GnC_Cy

Cuts at:      0     191     192    1071 
   Size:        191       1     879

  Fragments arranged by size: 

                 879     191       1
 
EcoRI G'AATT_C

Cuts at:      0     616     993    1071 
   Size:        616     377      78

  Fragments arranged by size: 

                 616     377      78
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     411    1071 
   Size:        411     660
 
HincII GTy'rAC

Cuts at:      0     501    1071 
   Size:        501     570
 
HindIII A'AGCT_T

Cuts at:      0     529    1071 
   Size:        529     542
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     764    1071 
   Size:        201     563     307

  Fragments arranged by size: 

                 563     307     201
 
MscI TGG'CCA

Cuts at:      0     320    1071 
   Size:        320     751
 
MslI CAynn'nnrTG

Cuts at:      0     329    1071 
   Size:        329     742
 
MspA1I CmG'CkG

Cuts at:      0     898    1071 
   Size:        898     173
 
NcoI C'CATG_G

Cuts at:      0     116     485    1071 
   Size:        116     369     586

  Fragments arranged by size: 

                 586     369     116
 
NdeI CA'TA_TG

Cuts at:      0     725    1071 
   Size:        725     346
 
NspI r_CATG'y

Cuts at:      0     590     832    1071 
   Size:        590     242     239

  Fragments arranged by size: 

                 590     242     239
 
PciI A'CATG_T

Cuts at:      0     586    1071 
   Size:        586     485
 
PfoI T'CCnGG_A

Cuts at:      0     458    1071 
   Size:        458     613
 
PsiI TTA'TAA

Cuts at:      0      25    1071 
   Size:         25    1046
 
PspOMI G'GGCC_C

Cuts at:      0     191    1071 
   Size:        191     880
 
ScaI AGT'ACT

Cuts at:      0     470    1071 
   Size:        470     601
 
SfcI C'TryA_G

Cuts at:      0     598     833    1071 
   Size:        598     235     238

  Fragments arranged by size: 

                 598     238     235
 
SphI G_CATG'C

Cuts at:      0     832    1071 
   Size:        832     239
 
SspI AAT'ATT

Cuts at:      0     948    1071 
   Size:        948     123
 
StyI C'CwwG_G

Cuts at:      0     116     485    1071 
   Size:        116     369     586

  Fragments arranged by size: 

                 586     369     116
 
TatI w'GTAC_w

Cuts at:      0     468    1071 
   Size:        468     603
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190     785     817    1071 
   Size:        158      32     595      32     254

  Fragments arranged by size: 

                 595     254     158      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     328     730    1071 
   Size:        328     402     341

  Fragments arranged by size: 

                 402     341     328
 
XmnI GAAnn'nnTTC

Cuts at:      0     779    1071 
   Size:        779     292
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     ApaI     ApoI     AseI    BanII
 Bme1580I     BsaI    BsaWI    BsaXI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspMI    BstBI    BstYI     BtgI     BtsI     DraI     EaeI     EarI
  Eco57MI EcoO109I    EcoRI     FalI   HincII  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NspI     PciI     PfoI     PsiI
   PspOMI     ScaI     SfcI     SphI     SspI     StyI     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseRI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI
     XmaI     ZraI