Restriction map for
pFN21AE1840
(Linear) (Six-base) MAPSORT of: KIEE1840.seq Check: 9324 from: 1 to: 1071
KIEE1840.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 676 1069 1071
Size: 676 393 2
Fragments arranged by size:
676 393 2
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 193 1071
Size: 175 18 878
Fragments arranged by size:
878 175 18
AfeI AGC'GCT
Cuts at: 0 289 408 424 1071
Size: 289 119 16 647
Fragments arranged by size:
647 289 119 16
AflIII A'CryG_T
Cuts at: 0 625 1071
Size: 625 446
AlwNI CAG_nnn'CTG
Cuts at: 0 711 869 1071
Size: 711 158 202
Fragments arranged by size:
711 202 158
ApaI G_GGCC'C
Cuts at: 0 150 789 1071
Size: 150 639 282
Fragments arranged by size:
639 282 150
AvaI C'yCGr_G
Cuts at: 0 395 917 1071
Size: 395 522 154
Fragments arranged by size:
522 395 154
BanII G_rGCy'C
Cuts at: 0 35 150 215 789 1071
Size: 35 115 65 574 282
Fragments arranged by size:
574 282 115 65 35
BglI GCCn_nnn'nGGC
Cuts at: 0 308 1071
Size: 308 763
Bme1580I G_kGCm'C
Cuts at: 0 121 150 564 789 1071
Size: 121 29 414 225 282
Fragments arranged by size:
414 282 225 121 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 472 1071
Size: 325 147 599
Fragments arranged by size:
599 325 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 310 481 1071
Size: 280 30 171 590
Fragments arranged by size:
590 280 171 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 1071
Size: 220 851
BsaAI yAC'GTr
Cuts at: 0 588 626 1020 1071
Size: 588 38 394 51
Fragments arranged by size:
588 394 51 38
BsaHI Gr'CG_yC
Cuts at: 0 100 572 1071
Size: 100 472 499
Fragments arranged by size:
499 472 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 1071
Size: 1039 30 2
Fragments arranged by size:
1039 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1060 1071
Size: 1060 11
BseYI C'CCAG_C
Cuts at: 0 409 862 877 1071
Size: 409 453 15 194
Fragments arranged by size:
453 409 194 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1024 1060 1071
Size: 411 613 36 11
Fragments arranged by size:
613 411 36 11
BsiHKAI G_wGCw'C
Cuts at: 0 916 1071
Size: 916 155
BsmBI CGTCTCn'nnnn_
Cuts at: 0 691 1071
Size: 691 380
Bsp1286I G_dGCh'C
Cuts at: 0 35 121 150 215 564 789 916 1071
Size: 35 86 29 65 349 225 127 155
Fragments arranged by size:
349 225 155 127 86 65 35 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 563 676 892 1069 1071
Size: 176 387 113 216 177 2
Fragments arranged by size:
387 216 177 176 113 2
BsrDI GCAATG_nn'
Cuts at: 0 1039 1071
Size: 1039 32
BsrFI r'CCGG_y
Cuts at: 0 458 872 1071
Size: 458 414 199
Fragments arranged by size:
458 414 199
BsrGI T'GTAC_A
Cuts at: 0 845 1055 1071
Size: 845 210 16
Fragments arranged by size:
845 210 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 941 1038 1071
Size: 941 97 33
Fragments arranged by size:
941 97 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 819 1071
Size: 819 252
Bsu36I CC'TnA_GG
Cuts at: 0 232 616 1071
Size: 232 384 455
Fragments arranged by size:
455 384 232
BtgI C'CryG_G
Cuts at: 0 636 1071
Size: 636 435
BtsI GCAGTG_nn'
Cuts at: 0 119 166 478 993 1012 1071
Size: 119 47 312 515 19 59
Fragments arranged by size:
515 312 119 59 47 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 733 1071
Size: 733 338
EaeI y'GGCC_r
Cuts at: 0 26 1071
Size: 26 1045
EarI CTCTTCn'nnn_
Cuts at: 0 601 1071
Size: 601 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 193 325 472 1071
Size: 175 18 132 147 599
Fragments arranged by size:
599 175 147 132 18
EcoO109I rG'GnC_Cy
Cuts at: 0 146 552 786 837 1071
Size: 146 406 234 51 234
Fragments arranged by size:
406 234 234 146 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 351 1071
Size: 351 720
FspI TGC'GCA
Cuts at: 0 245 664 1071
Size: 245 419 407
Fragments arranged by size:
419 407 245
FspAI rTGC'GCAy
Cuts at: 0 664 1071
Size: 664 407
HaeII r_GCGC'y
Cuts at: 0 49 291 410 426 1071
Size: 49 242 119 16 645
Fragments arranged by size:
645 242 119 49 16
MspA1I CmG'CkG
Cuts at: 0 929 1071
Size: 929 142
NaeI GCC'GGC
Cuts at: 0 460 874 1071
Size: 460 414 197
Fragments arranged by size:
460 414 197
NcoI C'CATG_G
Cuts at: 0 636 1071
Size: 636 435
NgoMIV G'CCGG_C
Cuts at: 0 458 872 1071
Size: 458 414 199
Fragments arranged by size:
458 414 199
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 832 1071
Size: 390 442 239
Fragments arranged by size:
442 390 239
PmlI CAC'GTG
Cuts at: 0 588 626 1071
Size: 588 38 445
Fragments arranged by size:
588 445 38
PpuMI rG'GwC_Cy
Cuts at: 0 552 837 1071
Size: 552 285 234
Fragments arranged by size:
552 285 234
PspOMI G'GGCC_C
Cuts at: 0 146 785 1071
Size: 146 639 286
Fragments arranged by size:
639 286 146
PstI C_TGCA'G
Cuts at: 0 406 934 1071
Size: 406 528 137
Fragments arranged by size:
528 406 137
PvuII CAG'CTG
Cuts at: 0 929 1071
Size: 929 142
ScaI AGT'ACT
Cuts at: 0 886 1071
Size: 886 185
SexAI A'CCwGG_T
Cuts at: 0 839 1071
Size: 839 232
SfcI C'TryA_G
Cuts at: 0 297 402 930 1071
Size: 297 105 528 141
Fragments arranged by size:
528 297 141 105
SmaI CCC'GGG
Cuts at: 0 397 919 1071
Size: 397 522 152
Fragments arranged by size:
522 397 152
SmlI C'TyrA_G
Cuts at: 0 259 325 496 1071
Size: 259 66 171 575
Fragments arranged by size:
575 259 171 66
SrfI GCCC'GGGC
Cuts at: 0 397 1071
Size: 397 674
StuI AGG'CCT
Cuts at: 0 466 763 818 1071
Size: 466 297 55 253
Fragments arranged by size:
466 297 253 55
StyI C'CwwG_G
Cuts at: 0 636 764 826 1071
Size: 636 128 62 245
Fragments arranged by size:
636 245 128 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 876 979 1071
Size: 397 479 103 92
Fragments arranged by size:
479 397 103 92
TatI w'GTAC_w
Cuts at: 0 500 845 884 1055 1071
Size: 500 345 39 171 16
Fragments arranged by size:
500 345 171 39 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 762 1071
Size: 762 309
XmaI C'CCGG_G
Cuts at: 0 395 917 1071
Size: 395 522 154
Fragments arranged by size:
522 395 154
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI BanII
BglI Bme1580I BpmI BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstXI Bsu36I BtgI BtsI DrdI
EaeI EarI Eco57MI EcoO109I FalI FspI FspAI HaeII
MspA1I NaeI NcoI NgoMIV PflMI PmlI PpuMI PspOMI
PstI PvuII ScaI SexAI SfcI SmaI SmlI SrfI
StuI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI