(Linear) (Six-base) MAPSORT of: KIEE1838.seq  Check: 5653  from: 1  to: 1062



KIEE1838.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:48  ..

AccI GT'mk_AC

Cuts at:      0     110    1062 
   Size:        110     952
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176    1062 
   Size:        176     886
 
ApoI r'AATT_y

Cuts at:      0     208     403     422     430     447     728     759    1062 
   Size:        208     195      19       8      17     281      31     303

  Fragments arranged by size: 

                 303     281     208     195      31      19      17       8
            
 
AvaI C'yCGr_G

Cuts at:      0     825    1062 
   Size:        825     237
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     782     815    1062 
   Size:        782      33     247

  Fragments arranged by size: 

                 782     247      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     783    1062 
   Size:        783     279
 
Bme1580I G_kGCm'C

Cuts at:      0     291    1062 
   Size:        291     771
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     609    1062 
   Size:        609     453
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     266    1062 
   Size:         75     191     796

  Fragments arranged by size: 

                 796     191      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1062 
   Size:        802     260
 
BseYI C'CCAG_C

Cuts at:      0      27    1062 
   Size:         27    1035
 
Bsp1286I G_dGCh'C

Cuts at:      0     291    1062 
   Size:        291     771
 
BspHI T'CATG_A

Cuts at:      0     723    1062 
   Size:        723     339
 
BsrFI r'CCGG_y

Cuts at:      0     519    1062 
   Size:        519     543
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     183    1062 
   Size:        183     879
 
BstYI r'GATC_y

Cuts at:      0     264    1062 
   Size:        264     798
 
BtsI GCAGTG_nn'

Cuts at:      0     120    1062 
   Size:        120     942
 
DraI TTT'AAA

Cuts at:      0     429    1062 
   Size:        429     633
 
EaeI y'GGCC_r

Cuts at:      0     858    1062 
   Size:        858     204
 
EarI CTCTTCn'nnn_

Cuts at:      0     874    1062 
   Size:        874     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     176     266    1062 
   Size:         75     101      90     796

  Fragments arranged by size: 

                 796     101      90      75
 
EcoRI G'AATT_C

Cuts at:      0     403    1062 
   Size:        403     659
 
HaeII r_GCGC'y

Cuts at:      0      46    1062 
   Size:         46    1016
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     793     808     825     840    1062 
   Size:        310      32     451      15      17      15     222

  Fragments arranged by size: 

                 451     310     222      32      17      15      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     495    1062 
   Size:        495     567
 
MscI TGG'CCA

Cuts at:      0     860    1062 
   Size:        860     202
 
MslI CAynn'nnrTG

Cuts at:      0     530     754     854    1062 
   Size:        530     224     100     208

  Fragments arranged by size: 

                 530     224     208     100
 
MspA1I CmG'CkG

Cuts at:      0     511    1062 
   Size:        511     551
 
PvuII CAG'CTG

Cuts at:      0     511    1062 
   Size:        511     551
 
SapI GCTCTTCn'nnn_

Cuts at:      0     874    1062 
   Size:        874     188
 
SfcI C'TryA_G

Cuts at:      0     107     618     941     950     992    1062 
   Size:        107     511     323       9      42      70

  Fragments arranged by size: 

                 511     323     107      70      42       9
 
SmlI C'TyrA_G

Cuts at:      0     781    1062 
   Size:        781     281
 
SspI AAT'ATT

Cuts at:      0     678    1062 
   Size:        678     384
 
StyI C'CwwG_G

Cuts at:      0     283     981    1062 
   Size:        283     698      81

  Fragments arranged by size: 

                 698     283      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     413    1062 
   Size:        413     649
 
XmnI GAAnn'nnTTC

Cuts at:      0     868    1062 
   Size:        868     194
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AvaI     BaeI    BciVI Bme1580I     BmrI
     BpmI    BpuEI    BseYI Bsp1286I    BspHI    BsrFI    BstXI    BstYI
     BtsI     DraI     EaeI     EarI  Eco57MI    EcoRI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I    PvuII     SapI     SfcI     SmlI
     SspI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI