Restriction map for
pFN21AE1834
(Linear) (Six-base) MAPSORT of: KIEE1834.seq Check: 975 from: 1 to: 1056
KIEE1834.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AccI GT'mk_AC
Cuts at: 0 744 1056
Size: 744 312
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1056
Size: 572 484
AleI CACnn'nnGTG
Cuts at: 0 889 1056
Size: 889 167
AlwNI CAG_nnn'CTG
Cuts at: 0 504 527 1056
Size: 504 23 529
Fragments arranged by size:
529 504 23
ApaI G_GGCC'C
Cuts at: 0 95 1056
Size: 95 961
AvaI C'yCGr_G
Cuts at: 0 668 1056
Size: 668 388
AvrII C'CTAG_G
Cuts at: 0 543 1056
Size: 543 513
BanI G'GyrC_C
Cuts at: 0 578 925 965 1056
Size: 578 347 40 91
Fragments arranged by size:
578 347 91 40
BanII G_rGCy'C
Cuts at: 0 95 625 943 1056
Size: 95 530 318 113
Fragments arranged by size:
530 318 113 95
BbeI G_GCGC'C
Cuts at: 0 969 1056
Size: 969 87
Bme1580I G_kGCm'C
Cuts at: 0 95 258 581 841 1056
Size: 95 163 323 260 215
Fragments arranged by size:
323 260 215 163 95
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 529 610 953 1056
Size: 127 402 81 343 103
Fragments arranged by size:
402 343 127 103 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 889 1056
Size: 542 347 167
Fragments arranged by size:
542 347 167
BsaHI Gr'CG_yC
Cuts at: 0 966 1056
Size: 966 90
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 345 1056
Size: 19 326 711
Fragments arranged by size:
711 326 19
BseYI C'CCAG_C
Cuts at: 0 173 462 1056
Size: 173 289 594
Fragments arranged by size:
594 289 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 206 433 1056
Size: 148 58 227 623
Fragments arranged by size:
623 227 148 58
BsiHKAI G_wGCw'C
Cuts at: 0 625 896 1056
Size: 625 271 160
Fragments arranged by size:
625 271 160
Bsp1286I G_dGCh'C
Cuts at: 0 95 258 581 625 841 896 943 1056
Size: 95 163 323 44 216 55 47 113
Fragments arranged by size:
323 216 163 113 95 55 47 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 1056
Size: 512 544
BsrDI GCAATG_nn'
Cuts at: 0 10 1056
Size: 10 1046
BstAPI GCAn_nnn'nTGC
Cuts at: 0 716 1056
Size: 716 340
Bsu36I CC'TnA_GG
Cuts at: 0 803 1056
Size: 803 253
BtgI C'CryG_G
Cuts at: 0 237 956 1056
Size: 237 719 100
Fragments arranged by size:
719 237 100
BtsI GCAGTG_nn'
Cuts at: 0 223 841 884 896 1056
Size: 223 618 43 12 160
Fragments arranged by size:
618 223 160 43 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 29 64 1056
Size: 29 35 992
Fragments arranged by size:
992 35 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 382 1056
Size: 382 674
EaeI y'GGCC_r
Cuts at: 0 50 137 1056
Size: 50 87 919
Fragments arranged by size:
919 87 50
EcoICRI GAG'CTC
Cuts at: 0 623 1056
Size: 623 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 529 572 610 953 1056
Size: 127 402 43 38 343 103
Fragments arranged by size:
402 343 127 103 43 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 508 1056
Size: 508 548
EcoO109I rG'GnC_Cy
Cuts at: 0 91 243 547 1056
Size: 91 152 304 509
Fragments arranged by size:
509 304 152 91
HaeII r_GCGC'y
Cuts at: 0 180 969 1056
Size: 180 789 87
Fragments arranged by size:
789 180 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 695 726 727 758 1056
Size: 695 31 1 31 298
Fragments arranged by size:
695 298 31 31 1
HincII GTy'rAC
Cuts at: 0 745 1056
Size: 745 311
KasI G'GCGC_C
Cuts at: 0 965 1056
Size: 965 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1056
Size: 64 992
MscI TGG'CCA
Cuts at: 0 52 139 1056
Size: 52 87 917
Fragments arranged by size:
917 87 52
MslI CAynn'nnrTG
Cuts at: 0 889 1056
Size: 889 167
MspA1I CmG'CkG
Cuts at: 0 117 919 1056
Size: 117 802 137
Fragments arranged by size:
802 137 117
NarI GG'CG_CC
Cuts at: 0 966 1056
Size: 966 90
NspI r_CATG'y
Cuts at: 0 86 861 1056
Size: 86 775 195
Fragments arranged by size:
775 195 86
PflMI CCAn_nnn'nTGG
Cuts at: 0 85 527 1056
Size: 85 442 529
Fragments arranged by size:
529 442 85
PfoI T'CCnGG_A
Cuts at: 0 730 1056
Size: 730 326
PpuMI rG'GwC_Cy
Cuts at: 0 243 1056
Size: 243 813
PspOMI G'GGCC_C
Cuts at: 0 91 1056
Size: 91 965
PstI C_TGCA'G
Cuts at: 0 230 571 891 924 1056
Size: 230 341 320 33 132
Fragments arranged by size:
341 320 230 132 33
PvuII CAG'CTG
Cuts at: 0 919 1056
Size: 919 137
SacI G_AGCT'C
Cuts at: 0 625 1056
Size: 625 431
SalI G'TCGA_C
Cuts at: 0 743 1056
Size: 743 313
SanDI GG'GwC_CC
Cuts at: 0 243 1056
Size: 243 813
SfcI C'TryA_G
Cuts at: 0 226 567 887 920 1056
Size: 226 341 320 33 136
Fragments arranged by size:
341 320 226 136 33
SfoI GGC'GCC
Cuts at: 0 967 1056
Size: 967 89
SmaI CCC'GGG
Cuts at: 0 670 1056
Size: 670 386
SmlI C'TyrA_G
Cuts at: 0 557 904 1056
Size: 557 347 152
Fragments arranged by size:
557 347 152
SphI G_CATG'C
Cuts at: 0 861 1056
Size: 861 195
StuI AGG'CCT
Cuts at: 0 314 654 1042 1056
Size: 314 340 388 14
Fragments arranged by size:
388 340 314 14
StyI C'CwwG_G
Cuts at: 0 98 488 543 720 1056
Size: 98 390 55 177 336
Fragments arranged by size:
390 336 177 98 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 625 1037 1056
Size: 187 438 412 19
Fragments arranged by size:
438 412 187 19
XbaI T'CTAG_A
Cuts at: 0 261 1056
Size: 261 795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 486 1056
Size: 102 384 570
Fragments arranged by size:
570 384 102
XmaI C'CCGG_G
Cuts at: 0 668 1056
Size: 668 388
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI AvaI AvrII BanI
BanII BbeI Bme1580I BpmI BpuEI BsaHI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI Bsu36I BtgI
BtsI CspCI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NarI NspI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SalI SanDI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraI
DraIII EagI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI TstI Tth111I XhoI XmnI ZraI