Restriction map for
pFN21AE1831
(Linear) (Six-base) MAPSORT of: KIEE1831.seq Check: 1929 from: 1 to: 1047
KIEE1831.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 686 699 1021 1047
Size: 686 13 322 26
Fragments arranged by size:
686 322 26 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1047
Size: 703 344
AflIII A'CryG_T
Cuts at: 0 32 1047
Size: 32 1015
AleI CACnn'nnGTG
Cuts at: 0 958 1047
Size: 958 89
ApoI r'AATT_y
Cuts at: 0 979 1047
Size: 979 68
AvaI C'yCGr_G
Cuts at: 0 506 1047
Size: 506 541
BanI G'GyrC_C
Cuts at: 0 530 1047
Size: 530 517
BanII G_rGCy'C
Cuts at: 0 290 1047
Size: 290 757
BbeI G_GCGC'C
Cuts at: 0 534 1047
Size: 534 513
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 274 1026 1047
Size: 267 7 752 21
Fragments arranged by size:
752 267 21 7
Bme1580I G_kGCm'C
Cuts at: 0 865 1047
Size: 865 182
BmgBI CAC'GTC
Cuts at: 0 465 890 1047
Size: 465 425 157
Fragments arranged by size:
465 425 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1047
Size: 613 434
Bpu10I CC'TnA_GC
Cuts at: 0 382 1047
Size: 382 665
BsaAI yAC'GTr
Cuts at: 0 33 60 320 1047
Size: 33 27 260 727
Fragments arranged by size:
727 260 33 27
BsaBI GATnn'nnATC
Cuts at: 0 205 1047
Size: 205 842
BsaHI Gr'CG_yC
Cuts at: 0 50 531 1047
Size: 50 481 516
Fragments arranged by size:
516 481 50
BsaWI w'CCGG_w
Cuts at: 0 581 1047
Size: 581 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 1047
Size: 586 30 431
Fragments arranged by size:
586 431 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 1047
Size: 850 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 727 1047
Size: 609 118 320
Fragments arranged by size:
609 320 118
BsiEI CG_ry'CG
Cuts at: 0 402 461 704 930 1047
Size: 402 59 243 226 117
Fragments arranged by size:
402 243 226 117 59
BsiHKAI G_wGCw'C
Cuts at: 0 75 290 1047
Size: 75 215 757
Fragments arranged by size:
757 215 75
BsiWI C'GTAC_G
Cuts at: 0 320 1047
Size: 320 727
BsmBI CGTCTCn'nnnn_
Cuts at: 0 880 1047
Size: 880 167
Bsp1286I G_dGCh'C
Cuts at: 0 75 290 865 1047
Size: 75 215 575 182
Fragments arranged by size:
575 215 182 75
BspHI T'CATG_A
Cuts at: 0 536 1047
Size: 536 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 551 646 686 699 1021 1047
Size: 551 95 40 13 322 26
Fragments arranged by size:
551 322 95 40 26 13
BsrFI r'CCGG_y
Cuts at: 0 559 1047
Size: 559 488
BsrGI T'GTAC_A
Cuts at: 0 806 1047
Size: 806 241
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1013 1047
Size: 1013 34
BtgI C'CryG_G
Cuts at: 0 83 136 332 606 788 848 1047
Size: 83 53 196 274 182 60 199
Fragments arranged by size:
274 199 196 182 83 60 53
BtsI GCAGTG_nn'
Cuts at: 0 973 1047
Size: 973 74
DraIII CAC_nnn'GTG
Cuts at: 0 612 920 1047
Size: 612 308 127
Fragments arranged by size:
612 308 127
EaeI y'GGCC_r
Cuts at: 0 458 701 845 927 1047
Size: 458 243 144 82 120
Fragments arranged by size:
458 243 144 120 82
EagI C'GGCC_G
Cuts at: 0 458 701 927 1047
Size: 458 243 226 120
Fragments arranged by size:
458 243 226 120
EcoICRI GAG'CTC
Cuts at: 0 288 1047
Size: 288 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 703 1047
Size: 613 90 344
Fragments arranged by size:
613 344 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 682 1047
Size: 682 365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 1047
Size: 291 756
FspI TGC'GCA
Cuts at: 0 757 772 1047
Size: 757 15 275
Fragments arranged by size:
757 275 15
HaeII r_GCGC'y
Cuts at: 0 495 534 1047
Size: 495 39 513
Fragments arranged by size:
513 495 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 1047
Size: 89 32 926
Fragments arranged by size:
926 89 32
KasI G'GCGC_C
Cuts at: 0 530 1047
Size: 530 517
MslI CAynn'nnrTG
Cuts at: 0 178 551 958 1047
Size: 178 373 407 89
Fragments arranged by size:
407 373 178 89
MspA1I CmG'CkG
Cuts at: 0 138 334 361 591 768 1047
Size: 138 196 27 230 177 279
Fragments arranged by size:
279 230 196 177 138 27
NaeI GCC'GGC
Cuts at: 0 561 1047
Size: 561 486
NarI GG'CG_CC
Cuts at: 0 531 1047
Size: 531 516
NcoI C'CATG_G
Cuts at: 0 788 1047
Size: 788 259
NgoMIV G'CCGG_C
Cuts at: 0 559 1047
Size: 559 488
PasI CC'CwG_GG
Cuts at: 0 93 1047
Size: 93 954
PflMI CCAn_nnn'nTGG
Cuts at: 0 962 1047
Size: 962 85
PstI C_TGCA'G
Cuts at: 0 370 1018 1047
Size: 370 648 29
Fragments arranged by size:
648 370 29
PvuII CAG'CTG
Cuts at: 0 591 768 1047
Size: 591 177 279
Fragments arranged by size:
591 279 177
SacI G_AGCT'C
Cuts at: 0 290 1047
Size: 290 757
SacII CC_GC'GG
Cuts at: 0 139 335 1047
Size: 139 196 712
Fragments arranged by size:
712 196 139
SexAI A'CCwGG_T
Cuts at: 0 800 1047
Size: 800 247
SfcI C'TryA_G
Cuts at: 0 366 1014 1047
Size: 366 648 33
Fragments arranged by size:
648 366 33
SfoI GGC'GCC
Cuts at: 0 532 1047
Size: 532 515
StyI C'CwwG_G
Cuts at: 0 372 788 956 1047
Size: 372 416 168 91
Fragments arranged by size:
416 372 168 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 958 1047
Size: 958 89
TatI w'GTAC_w
Cuts at: 0 692 806 950 1047
Size: 692 114 144 97
Fragments arranged by size:
692 144 114 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 1047
Size: 586 32 429
Fragments arranged by size:
586 429 32
XbaI T'CTAG_A
Cuts at: 0 573 1047
Size: 573 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 613 855 1047
Size: 613 242 192
Fragments arranged by size:
613 242 192
Enzymes that do cut:
AarI AcuI AflIII AleI ApoI AvaI BanI BanII
BbeI BbsI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BtgI
BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI FalI
FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PstI PvuII SacI SacII
SexAI SfcI SfoI StyI TaqII TatI TstI XbaI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpuEI BsaI
BseYI BsmI BspEI BsrBI BsrDI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EciI EcoO109I EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI
XmaI XmnI ZraI