Restriction map for
pFN21AE1829
(Linear) (Six-base) MAPSORT of: KIEE1829.seq Check: 9760 from: 1 to: 1041
KIEE1829.orf
With 182 enzymes: SgfI *
July 3, 2012 17:02 ..
AccI GT'mk_AC
Cuts at: 0 708 1041
Size: 708 333
AclI AA'CG_TT
Cuts at: 0 903 1041
Size: 903 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 508 1041
Size: 356 152 533
Fragments arranged by size:
533 356 152
AleI CACnn'nnGTG
Cuts at: 0 571 1041
Size: 571 470
AlwNI CAG_nnn'CTG
Cuts at: 0 894 1041
Size: 894 147
ApaI G_GGCC'C
Cuts at: 0 45 504 666 1041
Size: 45 459 162 375
Fragments arranged by size:
459 375 162 45
BanI G'GyrC_C
Cuts at: 0 701 1034 1041
Size: 701 333 7
Fragments arranged by size:
701 333 7
BanII G_rGCy'C
Cuts at: 0 45 278 322 504 655 666 787 1041
Size: 45 233 44 182 151 11 121 254
Fragments arranged by size:
254 233 182 151 121 45 44 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 955 1041
Size: 955 86
BfrBI ATG'CAT
Cuts at: 0 880 1041
Size: 880 161
BglI GCCn_nnn'nGGC
Cuts at: 0 40 1041
Size: 40 1001
BlpI GC'TnA_GC
Cuts at: 0 133 272 961 1041
Size: 133 139 689 80
Fragments arranged by size:
689 139 133 80
Bme1580I G_kGCm'C
Cuts at: 0 45 504 666 942 1041
Size: 45 459 162 276 99
Fragments arranged by size:
459 276 162 99 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 937 1041
Size: 937 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 526 551 1041
Size: 34 492 25 490
Fragments arranged by size:
492 490 34 25
Bpu10I CC'TnA_GC
Cuts at: 0 244 1041
Size: 244 797
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 871 1041
Size: 871 170
BseYI C'CCAG_C
Cuts at: 0 618 1041
Size: 618 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 1041
Size: 13 1028
BsiHKAI G_wGCw'C
Cuts at: 0 278 578 655 995 1041
Size: 278 300 77 340 46
Fragments arranged by size:
340 300 278 77 46
BsiWI C'GTAC_G
Cuts at: 0 88 1041
Size: 88 953
Bsp1286I G_dGCh'C
Cuts at: 0 45 278 322 504 578 655 666 787
Size: 45 233 44 182 74 77 11 121
Cuts at: 787 942 995 1041
Size: 155 53 46
Fragments arranged by size:
233 182 155 121 77 74 53 46
45 44 11
BsrBI CCG'CTC
Cuts at: 0 390 1041
Size: 390 651
BstEII G'GTnAC_C
Cuts at: 0 429 462 1041
Size: 429 33 579
Fragments arranged by size:
579 429 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 1041
Size: 300 741
BstYI r'GATC_y
Cuts at: 0 258 1041
Size: 258 783
BtgI C'CryG_G
Cuts at: 0 544 1041
Size: 544 497
BtsI GCAGTG_nn'
Cuts at: 0 9 74 206 1041
Size: 9 65 132 835
Fragments arranged by size:
835 132 65 9
EaeI y'GGCC_r
Cuts at: 0 71 541 1041
Size: 71 470 500
Fragments arranged by size:
500 470 71
EcoICRI GAG'CTC
Cuts at: 0 276 653 1041
Size: 276 377 388
Fragments arranged by size:
388 377 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 356 508 526 551 1041
Size: 34 322 152 18 25 490
Fragments arranged by size:
490 322 152 34 25 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 601 1041
Size: 49 552 440
Fragments arranged by size:
552 440 49
EcoO109I rG'GnC_Cy
Cuts at: 0 42 500 501 1041
Size: 42 458 1 540
Fragments arranged by size:
540 458 42 1
HaeII r_GCGC'y
Cuts at: 0 495 1041
Size: 495 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 690 722 1041
Size: 456 32 202 32 319
Fragments arranged by size:
456 319 202 32 32
HincII GTy'rAC
Cuts at: 0 709 733 1041
Size: 709 24 308
Fragments arranged by size:
709 308 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 721 892 1041
Size: 617 104 171 149
Fragments arranged by size:
617 171 149 104
MscI TGG'CCA
Cuts at: 0 73 1041
Size: 73 968
MslI CAynn'nnrTG
Cuts at: 0 571 1018 1041
Size: 571 447 23
Fragments arranged by size:
571 447 23
MspA1I CmG'CkG
Cuts at: 0 153 288 380 546 618 678 960 1041
Size: 153 135 92 166 72 60 282 81
Fragments arranged by size:
282 166 153 135 92 81 72 60
NsiI A_TGCA'T
Cuts at: 0 882 1041
Size: 882 159
NspI r_CATG'y
Cuts at: 0 132 969 1041
Size: 132 837 72
Fragments arranged by size:
837 132 72
PasI CC'CwG_GG
Cuts at: 0 496 1041
Size: 496 545
PfoI T'CCnGG_A
Cuts at: 0 787 1041
Size: 787 254
PshAI GACnn'nnGTC
Cuts at: 0 678 728 1041
Size: 678 50 313
Fragments arranged by size:
678 313 50
PspOMI G'GGCC_C
Cuts at: 0 41 500 662 1041
Size: 41 459 162 379
Fragments arranged by size:
459 379 162 41
PstI C_TGCA'G
Cuts at: 0 287 1041
Size: 287 754
PvuII CAG'CTG
Cuts at: 0 288 380 618 678 1041
Size: 288 92 238 60 363
Fragments arranged by size:
363 288 238 92 60
SacI G_AGCT'C
Cuts at: 0 278 655 1041
Size: 278 377 386
Fragments arranged by size:
386 377 278
SacII CC_GC'GG
Cuts at: 0 547 1041
Size: 547 494
SalI G'TCGA_C
Cuts at: 0 707 1041
Size: 707 334
SfcI C'TryA_G
Cuts at: 0 181 283 1041
Size: 181 102 758
Fragments arranged by size:
758 181 102
SphI G_CATG'C
Cuts at: 0 969 1041
Size: 969 72
StuI AGG'CCT
Cuts at: 0 471 1041
Size: 471 570
StyI C'CwwG_G
Cuts at: 0 440 1041
Size: 440 601
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1022 1041
Size: 610 412 19
Fragments arranged by size:
610 412 19
TatI w'GTAC_w
Cuts at: 0 809 1041
Size: 809 232
Enzymes that do cut:
AccI AclI AcuI AleI AlwNI ApaI BanI BanII
BbsI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I BsrBI BstEII
BstXI BstYI BtgI BtsI EaeI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I
NsiI NspI PasI PfoI PshAI PspOMI PstI PvuII
SacI SacII SalI SfcI SphI StuI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI