Restriction map for
pFN21AE1828
(Linear) (Six-base) MAPSORT of: KIEE1828.seq Check: 6118 from: 1 to: 1038
KIEE1828.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AclI AA'CG_TT
Cuts at: 0 221 1038
Size: 221 817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 261 1038
Size: 51 210 777
Fragments arranged by size:
777 210 51
AflIII A'CryG_T
Cuts at: 0 362 1038
Size: 362 676
AleI CACnn'nnGTG
Cuts at: 0 214 1038
Size: 214 824
AlwNI CAG_nnn'CTG
Cuts at: 0 89 656 1038
Size: 89 567 382
Fragments arranged by size:
567 382 89
ApoI r'AATT_y
Cuts at: 0 10 115 930 1038
Size: 10 105 815 108
Fragments arranged by size:
815 108 105 10
BamHI G'GATC_C
Cuts at: 0 467 488 1038
Size: 467 21 550
Fragments arranged by size:
550 467 21
BanI G'GyrC_C
Cuts at: 0 61 1038
Size: 61 977
BclI T'GATC_A
Cuts at: 0 668 758 1038
Size: 668 90 280
Fragments arranged by size:
668 280 90
BglII A'GATC_T
Cuts at: 0 249 1038
Size: 249 789
BlpI GC'TnA_GC
Cuts at: 0 587 1038
Size: 587 451
BsaI GGTCTCn'nnnn_
Cuts at: 0 314 1038
Size: 314 724
BsaBI GATnn'nnATC
Cuts at: 0 493 1038
Size: 493 545
BseYI C'CCAG_C
Cuts at: 0 922 1038
Size: 922 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 938 1038
Size: 75 863 100
Fragments arranged by size:
863 100 75
BsrDI GCAATG_nn'
Cuts at: 0 422 1038
Size: 422 616
BsrGI T'GTAC_A
Cuts at: 0 365 1038
Size: 365 673
BstAPI GCAn_nnn'nTGC
Cuts at: 0 89 889 1038
Size: 89 800 149
Fragments arranged by size:
800 149 89
BstYI r'GATC_y
Cuts at: 0 249 467 488 1038
Size: 249 218 21 550
Fragments arranged by size:
550 249 218 21
BtsI GCAGTG_nn'
Cuts at: 0 552 1038
Size: 552 486
EaeI y'GGCC_r
Cuts at: 0 239 1038
Size: 239 799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 261 1038
Size: 51 210 777
Fragments arranged by size:
777 210 51
EcoO109I rG'GnC_Cy
Cuts at: 0 696 1038
Size: 696 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 1038
Size: 514 32 492
Fragments arranged by size:
514 492 32
HincII GTy'rAC
Cuts at: 0 360 1038
Size: 360 678
HindIII A'AGCT_T
Cuts at: 0 854 1038
Size: 854 184
MfeI C'AATT_G
Cuts at: 0 95 125 306 1038
Size: 95 30 181 732
Fragments arranged by size:
732 181 95 30
MscI TGG'CCA
Cuts at: 0 241 1038
Size: 241 797
MslI CAynn'nnrTG
Cuts at: 0 214 1038
Size: 214 824
NdeI CA'TA_TG
Cuts at: 0 484 1038
Size: 484 554
NspI r_CATG'y
Cuts at: 0 366 445 1038
Size: 366 79 593
Fragments arranged by size:
593 366 79
PciI A'CATG_T
Cuts at: 0 362 1038
Size: 362 676
PfoI T'CCnGG_A
Cuts at: 0 518 1038
Size: 518 520
PpuMI rG'GwC_Cy
Cuts at: 0 696 1038
Size: 696 342
PshAI GACnn'nnGTC
Cuts at: 0 948 1038
Size: 948 90
PsiI TTA'TAA
Cuts at: 0 200 1038
Size: 200 838
PstI C_TGCA'G
Cuts at: 0 194 334 596 1038
Size: 194 140 262 442
Fragments arranged by size:
442 262 194 140
SbfI CC_TGCA'GG
Cuts at: 0 334 1038
Size: 334 704
SfcI C'TryA_G
Cuts at: 0 190 330 592 1038
Size: 190 140 262 446
Fragments arranged by size:
446 262 190 140
SspI AAT'ATT
Cuts at: 0 143 1038
Size: 143 895
StyI C'CwwG_G
Cuts at: 0 614 875 1038
Size: 614 261 163
Fragments arranged by size:
614 261 163
TatI w'GTAC_w
Cuts at: 0 365 1038
Size: 365 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 459 491 1038
Size: 459 32 547
Fragments arranged by size:
547 459 32
XmnI GAAnn'nnTTC
Cuts at: 0 223 1038
Size: 223 815
Enzymes that do cut:
AclI AcuI AflIII AleI AlwNI ApoI BamHI BanI
BclI BglII BlpI BsaI BsaBI BseYI BsgI BsrDI
BsrGI BstAPI BstYI BtsI EaeI Eco57MI EcoO109I Hin4I
HincII HindIII MfeI MscI MslI NdeI NspI PciI
PfoI PpuMI PshAI PsiI PstI SbfI SfcI SspI
StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI